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Multiplex PCR to discriminate bovine, porcine, and fish DNA in gelatin and confectionery products

Gelatin is widely used in many confectionery products such as gummy, candies, pastilles and marshmallows. However, the acceptance of gelatin in consumer goods largely depends on its animal origins. The main sources of gelatin are pig, cow and fish collagens that have differential acceptance in Halal...

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Main Authors:	Sultana, Sharmin, Hossain, Motalib A.Motalib, Sarker, Md. Zaidul Islam, Ali, Md Eaqub
Format:	Article
Language:	English English English
Published:	Academic Press Inc. 2018
Subjects:	RS Pharmacy and materia medica RS403 Materia Medica-Pharmaceutical Chemistry TP Chemical technology
Online Access:	http://irep.iium.edu.my/63897/2/63897_Multiplex%20PCR%20to%20discriminate%20bovine%2C%20porcine%2C%20and%20fish%20DNA%20in%20gelatin_scopus.pdf http://irep.iium.edu.my/63897/13/63897_Multiplex%20PCR%20to%20discriminate%20bovine%2C%20porcine.pdf http://irep.iium.edu.my/63897/19/63897_Multiplex%20PCR%20to%20discriminate%20bovine_WoS.pdf http://irep.iium.edu.my/63897/ https://www.sciencedirect.com/science/article/pii/S0023643818301488?via%3Dihub
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spelling	my.iium.irep.638972018-11-01T08:46:04Z http://irep.iium.edu.my/63897/ Multiplex PCR to discriminate bovine, porcine, and fish DNA in gelatin and confectionery products Sultana, Sharmin Hossain, Motalib A.Motalib Sarker, Md. Zaidul Islam Ali, Md Eaqub RS Pharmacy and materia medica RS403 Materia Medica-Pharmaceutical Chemistry TP Chemical technology Gelatin is widely used in many confectionery products such as gummy, candies, pastilles and marshmallows. However, the acceptance of gelatin in consumer goods largely depends on its animal origins. The main sources of gelatin are pig, cow and fish collagens that have differential acceptance in Halal, Kosher and Hindu foods. Currently, several simplex and duplex PCR assays are available for the determination of gelatin origins but those methods are not only time-consuming and costly but also cannot reveal all information in a single assay platform. For the first time, we documented here a tetraplex PCR assay involving eukaryotic, fish, bovine, and porcine control for the simultaneous detection of these species by a single, easy and reliable approach. The assay was optimized under pure and mixed gelatin matrices and validated by PCR product sequencing. Finally, 38 halal branded confectionery items were screened and 33, 2 and 3 of them yielded positive bands for bovine, porcine and eukaryotes, respectively. The detection limit was 0.1 to 0.001 ng DNA extracted from pure and mixed gelatins. The study is potentially useful in monitoring and certifying halal, kosher and vegetarian issues in processed foods, pharmaceuticals and cosmetics. Academic Press Inc. 2018-06 Article PeerReviewed application/pdf en http://irep.iium.edu.my/63897/2/63897_Multiplex%20PCR%20to%20discriminate%20bovine%2C%20porcine%2C%20and%20fish%20DNA%20in%20gelatin_scopus.pdf application/pdf en http://irep.iium.edu.my/63897/13/63897_Multiplex%20PCR%20to%20discriminate%20bovine%2C%20porcine.pdf application/pdf en http://irep.iium.edu.my/63897/19/63897_Multiplex%20PCR%20to%20discriminate%20bovine_WoS.pdf Sultana, Sharmin and Hossain, Motalib A.Motalib and Sarker, Md. Zaidul Islam and Ali, Md Eaqub (2018) Multiplex PCR to discriminate bovine, porcine, and fish DNA in gelatin and confectionery products. LWT - Food Science and Technology, 92. ISSN 0023-6438 https://www.sciencedirect.com/science/article/pii/S0023643818301488?via%3Dihub 10.1016/j.lwt.2018.02.019
institution	Universiti Islam Antarabangsa Malaysia
building	IIUM Library
collection	Institutional Repository
continent	Asia
country	Malaysia
content_provider	International Islamic University Malaysia
content_source	IIUM Repository (IREP)
url_provider	http://irep.iium.edu.my/
language	English English English
topic	RS Pharmacy and materia medica RS403 Materia Medica-Pharmaceutical Chemistry TP Chemical technology
spellingShingle	RS Pharmacy and materia medica RS403 Materia Medica-Pharmaceutical Chemistry TP Chemical technology Sultana, Sharmin Hossain, Motalib A.Motalib Sarker, Md. Zaidul Islam Ali, Md Eaqub Multiplex PCR to discriminate bovine, porcine, and fish DNA in gelatin and confectionery products
description	Gelatin is widely used in many confectionery products such as gummy, candies, pastilles and marshmallows. However, the acceptance of gelatin in consumer goods largely depends on its animal origins. The main sources of gelatin are pig, cow and fish collagens that have differential acceptance in Halal, Kosher and Hindu foods. Currently, several simplex and duplex PCR assays are available for the determination of gelatin origins but those methods are not only time-consuming and costly but also cannot reveal all information in a single assay platform. For the first time, we documented here a tetraplex PCR assay involving eukaryotic, fish, bovine, and porcine control for the simultaneous detection of these species by a single, easy and reliable approach. The assay was optimized under pure and mixed gelatin matrices and validated by PCR product sequencing. Finally, 38 halal branded confectionery items were screened and 33, 2 and 3 of them yielded positive bands for bovine, porcine and eukaryotes, respectively. The detection limit was 0.1 to 0.001 ng DNA extracted from pure and mixed gelatins. The study is potentially useful in monitoring and certifying halal, kosher and vegetarian issues in processed foods, pharmaceuticals and cosmetics.
format	Article
author	Sultana, Sharmin Hossain, Motalib A.Motalib Sarker, Md. Zaidul Islam Ali, Md Eaqub
author_facet	Sultana, Sharmin Hossain, Motalib A.Motalib Sarker, Md. Zaidul Islam Ali, Md Eaqub
author_sort	Sultana, Sharmin
title	Multiplex PCR to discriminate bovine, porcine, and fish DNA in gelatin and confectionery products
title_short	Multiplex PCR to discriminate bovine, porcine, and fish DNA in gelatin and confectionery products
title_full	Multiplex PCR to discriminate bovine, porcine, and fish DNA in gelatin and confectionery products
title_fullStr	Multiplex PCR to discriminate bovine, porcine, and fish DNA in gelatin and confectionery products
title_full_unstemmed	Multiplex PCR to discriminate bovine, porcine, and fish DNA in gelatin and confectionery products
title_sort	multiplex pcr to discriminate bovine, porcine, and fish dna in gelatin and confectionery products
publisher	Academic Press Inc.
publishDate	2018
url	http://irep.iium.edu.my/63897/2/63897_Multiplex%20PCR%20to%20discriminate%20bovine%2C%20porcine%2C%20and%20fish%20DNA%20in%20gelatin_scopus.pdf http://irep.iium.edu.my/63897/13/63897_Multiplex%20PCR%20to%20discriminate%20bovine%2C%20porcine.pdf http://irep.iium.edu.my/63897/19/63897_Multiplex%20PCR%20to%20discriminate%20bovine_WoS.pdf http://irep.iium.edu.my/63897/ https://www.sciencedirect.com/science/article/pii/S0023643818301488?via%3Dihub
_version_	1643617854406262784
score	13.211869

Multiplex PCR to discriminate bovine, porcine, and fish DNA in gelatin and confectionery products

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