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Extraction and purification of cytotoxic compounds from Premna serratifolia L. (bebuas) for human breast cancer treatment

Breast cancer is considered as one of the most common cancers all over the world. A huge effort has been made to create a safe and cost effective breast cancer treatment. All of these features exist in the plants sources. In this study, the effect of local vegetable salad, Premna serratifolia (Be...

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Main Authors: Salih, Ghassan Ali, Ahmad Raus, Raha, Shaban, Mustaffa Nameer, Abdullah, N.
Format: Article
Language:English
English
Published: Faculty of Food Science & Technology, Universiti Putra Malaysia (UPM) 2017
Subjects:
QD Chemistry
QK Botany
RC0254 Neoplasms. Tumors. Oncology (including Cancer)
TP248.13 Biotechnology
Online Access:http://irep.iium.edu.my/57590/1/57590_Extraction%20and%20purification%20of%20cytotoxic.pdf
http://irep.iium.edu.my/57590/7/57590_Extraction%20and%20purification%20of%20cytotoxic%20compounds_scopus.pdf
http://irep.iium.edu.my/57590/
http://www.ifrj.upm.edu.my/24%20(07)%202017%20supplementary/(4)%20R1.pdf
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spelling my.iium.irep.575902018-11-30T03:26:47Z http://irep.iium.edu.my/57590/ Extraction and purification of cytotoxic compounds from Premna serratifolia L. (bebuas) for human breast cancer treatment Salih, Ghassan Ali Ahmad Raus, Raha Shaban, Mustaffa Nameer Abdullah, N. QD Chemistry QK Botany RC0254 Neoplasms. Tumors. Oncology (including Cancer) TP248.13 Biotechnology Breast cancer is considered as one of the most common cancers all over the world. A huge effort has been made to create a safe and cost effective breast cancer treatment. All of these features exist in the plants sources. In this study, the effect of local vegetable salad, Premna serratifolia (Bebuas) against MCF-7 cells (human breast adenocarcinoma) was determined. The optimum condition to extract breast cancer cytotoxic compound from the plant was investigated and the exact cytotoxic compound was identified as well. To determine the plant cytotoxicity effect against MCF-7 cells, MTT assay was used. Two important parameters in the sonication extraction method which are duration of time and temperature were optimized by carrying out a series of experiments which were designed by Face Centered Central Composite Design (FCCCD). The extraction efficiency of each experiment was determined by measuring the yield of extract and the half maximal inhibitory concentration (IC50) of the extract against MCF-7 cells. The results obtained from the experiments were fitted to the second order polynomial model to generate equation that was used to determine best extraction processing condition. Based on the generated equation, the best sonication processing condition to extract the cytotoxic compound is at 30o C for 67 min. Analysis of variance (ANOVA) showed that the duration of extraction time has great influence (p<0.05) on the yield of crude extract, while both parameters, time and temperature significantly (p<0.05) affected the IC50 value of the plant extract. Two purified compounds from the plant, acacetin and acacetin-7-O-glycoside were identified to be responsible for the cytotoxicity activity. Conclusively, in terms of pharmaceutical and medical field applications; the optimal extraction processing condition obtained from this study ensures that the extraction of cytotoxic compound from the plant is conducted at the low temperature and short duration of time. Further study on the identified compounds is required as it is a potential candidate for anticancer drug. Faculty of Food Science & Technology, Universiti Putra Malaysia (UPM) 2017-12 Article PeerReviewed application/pdf en http://irep.iium.edu.my/57590/1/57590_Extraction%20and%20purification%20of%20cytotoxic.pdf application/pdf en http://irep.iium.edu.my/57590/7/57590_Extraction%20and%20purification%20of%20cytotoxic%20compounds_scopus.pdf Salih, Ghassan Ali and Ahmad Raus, Raha and Shaban, Mustaffa Nameer and Abdullah, N. (2017) Extraction and purification of cytotoxic compounds from Premna serratifolia L. (bebuas) for human breast cancer treatment. International Food Research Journal, 24 (Suppl.). pp. 281-286. ISSN 1985-4668 http://www.ifrj.upm.edu.my/24%20(07)%202017%20supplementary/(4)%20R1.pdf
institution Universiti Islam Antarabangsa Malaysia
building IIUM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider International Islamic University Malaysia
content_source IIUM Repository (IREP)
url_provider http://irep.iium.edu.my/
language English
English
topic QD Chemistry
QK Botany
RC0254 Neoplasms. Tumors. Oncology (including Cancer)
TP248.13 Biotechnology
spellingShingle QD Chemistry
QK Botany
RC0254 Neoplasms. Tumors. Oncology (including Cancer)
TP248.13 Biotechnology
Salih, Ghassan Ali
Ahmad Raus, Raha
Shaban, Mustaffa Nameer
Abdullah, N.
Extraction and purification of cytotoxic compounds from Premna serratifolia L. (bebuas) for human breast cancer treatment
description Breast cancer is considered as one of the most common cancers all over the world. A huge effort has been made to create a safe and cost effective breast cancer treatment. All of these features exist in the plants sources. In this study, the effect of local vegetable salad, Premna serratifolia (Bebuas) against MCF-7 cells (human breast adenocarcinoma) was determined. The optimum condition to extract breast cancer cytotoxic compound from the plant was investigated and the exact cytotoxic compound was identified as well. To determine the plant cytotoxicity effect against MCF-7 cells, MTT assay was used. Two important parameters in the sonication extraction method which are duration of time and temperature were optimized by carrying out a series of experiments which were designed by Face Centered Central Composite Design (FCCCD). The extraction efficiency of each experiment was determined by measuring the yield of extract and the half maximal inhibitory concentration (IC50) of the extract against MCF-7 cells. The results obtained from the experiments were fitted to the second order polynomial model to generate equation that was used to determine best extraction processing condition. Based on the generated equation, the best sonication processing condition to extract the cytotoxic compound is at 30o C for 67 min. Analysis of variance (ANOVA) showed that the duration of extraction time has great influence (p<0.05) on the yield of crude extract, while both parameters, time and temperature significantly (p<0.05) affected the IC50 value of the plant extract. Two purified compounds from the plant, acacetin and acacetin-7-O-glycoside were identified to be responsible for the cytotoxicity activity. Conclusively, in terms of pharmaceutical and medical field applications; the optimal extraction processing condition obtained from this study ensures that the extraction of cytotoxic compound from the plant is conducted at the low temperature and short duration of time. Further study on the identified compounds is required as it is a potential candidate for anticancer drug.
format Article
author Salih, Ghassan Ali
Ahmad Raus, Raha
Shaban, Mustaffa Nameer
Abdullah, N.
author_facet Salih, Ghassan Ali
Ahmad Raus, Raha
Shaban, Mustaffa Nameer
Abdullah, N.
author_sort Salih, Ghassan Ali
title Extraction and purification of cytotoxic compounds from Premna serratifolia L. (bebuas) for human breast cancer treatment
title_short Extraction and purification of cytotoxic compounds from Premna serratifolia L. (bebuas) for human breast cancer treatment
title_full Extraction and purification of cytotoxic compounds from Premna serratifolia L. (bebuas) for human breast cancer treatment
title_fullStr Extraction and purification of cytotoxic compounds from Premna serratifolia L. (bebuas) for human breast cancer treatment
title_full_unstemmed Extraction and purification of cytotoxic compounds from Premna serratifolia L. (bebuas) for human breast cancer treatment
title_sort extraction and purification of cytotoxic compounds from premna serratifolia l. (bebuas) for human breast cancer treatment
publisher Faculty of Food Science & Technology, Universiti Putra Malaysia (UPM)
publishDate 2017
url http://irep.iium.edu.my/57590/1/57590_Extraction%20and%20purification%20of%20cytotoxic.pdf
http://irep.iium.edu.my/57590/7/57590_Extraction%20and%20purification%20of%20cytotoxic%20compounds_scopus.pdf
http://irep.iium.edu.my/57590/
http://www.ifrj.upm.edu.my/24%20(07)%202017%20supplementary/(4)%20R1.pdf
_version_ 1643617768021426176
score 13.222552

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