Extraction and purification of cytotoxic compounds from Premna serratifolia L. (bebuas) for human breast cancer treatment
Breast cancer is considered as one of the most common cancers all over the world. A huge effort has been made to create a safe and cost effective breast cancer treatment. All of these features exist in the plants sources. In this study, the effect of local vegetable salad, Premna serratifolia (Be...
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| Main Authors: | , , , |
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| Format: | Article |
| Language: | English English |
| Published: |
Faculty of Food Science & Technology, Universiti Putra Malaysia (UPM)
2017
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| Subjects: | |
| Online Access: | http://irep.iium.edu.my/57590/1/57590_Extraction%20and%20purification%20of%20cytotoxic.pdf http://irep.iium.edu.my/57590/7/57590_Extraction%20and%20purification%20of%20cytotoxic%20compounds_scopus.pdf http://irep.iium.edu.my/57590/ http://www.ifrj.upm.edu.my/24%20(07)%202017%20supplementary/(4)%20R1.pdf |
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| Summary: | Breast cancer is considered as one of the most common cancers all over the world. A huge
effort has been made to create a safe and cost effective breast cancer treatment. All of these
features exist in the plants sources. In this study, the effect of local vegetable salad, Premna
serratifolia (Bebuas) against MCF-7 cells (human breast adenocarcinoma) was determined.
The optimum condition to extract breast cancer cytotoxic compound from the plant was
investigated and the exact cytotoxic compound was identified as well. To determine the plant
cytotoxicity effect against MCF-7 cells, MTT assay was used. Two important parameters in the
sonication extraction method which are duration of time and temperature were optimized by
carrying out a series of experiments which were designed by Face Centered Central Composite
Design (FCCCD). The extraction efficiency of each experiment was determined by measuring
the yield of extract and the half maximal inhibitory concentration (IC50) of the extract against
MCF-7 cells. The results obtained from the experiments were fitted to the second order
polynomial model to generate equation that was used to determine best extraction processing
condition. Based on the generated equation, the best sonication processing condition to extract
the cytotoxic compound is at 30o
C for 67 min. Analysis of variance (ANOVA) showed that the
duration of extraction time has great influence (p<0.05) on the yield of crude extract, while
both parameters, time and temperature significantly (p<0.05) affected the IC50 value of the
plant extract. Two purified compounds from the plant, acacetin and acacetin-7-O-glycoside
were identified to be responsible for the cytotoxicity activity. Conclusively, in terms of
pharmaceutical and medical field applications; the optimal extraction processing condition
obtained from this study ensures that the extraction of cytotoxic compound from the plant is
conducted at the low temperature and short duration of time. Further study on the identified
compounds is required as it is a potential candidate for anticancer drug. |
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