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Purification and characterization of extracellular lipase from Candida cylindracea using palm oil mill effluent supplemented medium

Production of Candida cylindracea lipase was optimized and highest lipase production was achieved after 36 hr of fermentation using palm oil mill effluent supplemented medium. The extracellular lipase was purified by DEAE-sepharose and Sephacryl S-200, exhibiting a single band with molecular mass of...

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Main Authors: Alam, Md. Zahangir, Salihu, Aliyu, Abdul Karim, Mohamed Ismail, Hamzah, Mohd. Salleh
Format: Conference or Workshop Item
Language:English
Published: 2011
Subjects:
TP Chemical technology
TP248.13 Biotechnology
Online Access:http://irep.iium.edu.my/5629/1/article_SOMChe-ICCEIB_2011.pdf
http://irep.iium.edu.my/5629/
http://icceib.ump.edu.my/
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spelling my.iium.irep.56292021-06-23T06:51:35Z http://irep.iium.edu.my/5629/ Purification and characterization of extracellular lipase from Candida cylindracea using palm oil mill effluent supplemented medium Alam, Md. Zahangir Salihu, Aliyu Abdul Karim, Mohamed Ismail Hamzah, Mohd. Salleh TP Chemical technology TP248.13 Biotechnology Production of Candida cylindracea lipase was optimized and highest lipase production was achieved after 36 hr of fermentation using palm oil mill effluent supplemented medium. The extracellular lipase was purified by DEAE-sepharose and Sephacryl S-200, exhibiting a single band with molecular mass of 58 kDa by SDS-PAGE and gel filtration. The temperature and pH optima of the enzyme were found to be 35oC and 8 respectively. The enzyme was stable at pH 7.0 and 8.0 for 12 hr and also at temperature range of 25 to 35oC for 1 hr. Among the divalent cation salts tested on the lipase activity, Ca2+ and Mn2+ activated the enzyme most, while Cu2+ and Fe2+ inhibited it. Non-ionic (Tween-80 and Tween-20 and Triton X-100) and ionic detergents (SDS) at 1% appeared to have stimulatory and inhibitory effects on lipase activity respectively. In case of stability in organic solvents, water miscible solvents are concentration dependent; where their low concentrations showed some stimulatory effects, with marked inhibition at higher concentrations. It can be concluded that knowledge of these properties of the enzyme as described in this study could go a long way in its effective utilization for industrial application processes. 2011-11-28 Conference or Workshop Item PeerReviewed application/pdf en http://irep.iium.edu.my/5629/1/article_SOMChe-ICCEIB_2011.pdf Alam, Md. Zahangir and Salihu, Aliyu and Abdul Karim, Mohamed Ismail and Hamzah, Mohd. Salleh (2011) Purification and characterization of extracellular lipase from Candida cylindracea using palm oil mill effluent supplemented medium. In: International Conference of Chemical Engineering and Industrial Biotechnology (SOMChE-ICCEIB 2011), 28th Nov -1st Dec 2011, Teluk Cempedak, Kuantan. (In Press) http://icceib.ump.edu.my/
institution Universiti Islam Antarabangsa Malaysia
building IIUM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider International Islamic University Malaysia
content_source IIUM Repository (IREP)
url_provider http://irep.iium.edu.my/
language English
topic TP Chemical technology
TP248.13 Biotechnology
spellingShingle TP Chemical technology
TP248.13 Biotechnology
Alam, Md. Zahangir
Salihu, Aliyu
Abdul Karim, Mohamed Ismail
Hamzah, Mohd. Salleh
Purification and characterization of extracellular lipase from Candida cylindracea using palm oil mill effluent supplemented medium
description Production of Candida cylindracea lipase was optimized and highest lipase production was achieved after 36 hr of fermentation using palm oil mill effluent supplemented medium. The extracellular lipase was purified by DEAE-sepharose and Sephacryl S-200, exhibiting a single band with molecular mass of 58 kDa by SDS-PAGE and gel filtration. The temperature and pH optima of the enzyme were found to be 35oC and 8 respectively. The enzyme was stable at pH 7.0 and 8.0 for 12 hr and also at temperature range of 25 to 35oC for 1 hr. Among the divalent cation salts tested on the lipase activity, Ca2+ and Mn2+ activated the enzyme most, while Cu2+ and Fe2+ inhibited it. Non-ionic (Tween-80 and Tween-20 and Triton X-100) and ionic detergents (SDS) at 1% appeared to have stimulatory and inhibitory effects on lipase activity respectively. In case of stability in organic solvents, water miscible solvents are concentration dependent; where their low concentrations showed some stimulatory effects, with marked inhibition at higher concentrations. It can be concluded that knowledge of these properties of the enzyme as described in this study could go a long way in its effective utilization for industrial application processes.
format Conference or Workshop Item
author Alam, Md. Zahangir
Salihu, Aliyu
Abdul Karim, Mohamed Ismail
Hamzah, Mohd. Salleh
author_facet Alam, Md. Zahangir
Salihu, Aliyu
Abdul Karim, Mohamed Ismail
Hamzah, Mohd. Salleh
author_sort Alam, Md. Zahangir
title Purification and characterization of extracellular lipase from Candida cylindracea using palm oil mill effluent supplemented medium
title_short Purification and characterization of extracellular lipase from Candida cylindracea using palm oil mill effluent supplemented medium
title_full Purification and characterization of extracellular lipase from Candida cylindracea using palm oil mill effluent supplemented medium
title_fullStr Purification and characterization of extracellular lipase from Candida cylindracea using palm oil mill effluent supplemented medium
title_full_unstemmed Purification and characterization of extracellular lipase from Candida cylindracea using palm oil mill effluent supplemented medium
title_sort purification and characterization of extracellular lipase from candida cylindracea using palm oil mill effluent supplemented medium
publishDate 2011
url http://irep.iium.edu.my/5629/1/article_SOMChe-ICCEIB_2011.pdf
http://irep.iium.edu.my/5629/
http://icceib.ump.edu.my/
_version_ 1703960126153555968
score 13.211869

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