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Improvement of halophilic cellulase production from locally isolated fungal strain

Halophilic cellulases from the newly isolated fungus, Aspergillus terreus UniMAP AA-6 were found to be useful for in situ saccharification of ionic liquids treated lignocelluloses. Efforts have been taken to improve the enzyme production through statistical optimization approach namely Plackett–B...

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Main Authors: Nagoor Gunny, Ahmad Anas, Arbain, Dachyar, Jamal, Parveen, Gumba, Rizo Edwin
Format: Article
Language:English
Published: Elsevier Ltd. 2015
Subjects:
T Technology (General)
Online Access:http://irep.iium.edu.my/44276/1/Saudi_paper.pdf
http://irep.iium.edu.my/44276/
http://www.sciencedirect.com/science/article/pii/S1319562X14001673#
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spelling my.iium.irep.442762015-10-11T13:15:11Z http://irep.iium.edu.my/44276/ Improvement of halophilic cellulase production from locally isolated fungal strain Nagoor Gunny, Ahmad Anas Arbain, Dachyar Jamal, Parveen Gumba, Rizo Edwin T Technology (General) Halophilic cellulases from the newly isolated fungus, Aspergillus terreus UniMAP AA-6 were found to be useful for in situ saccharification of ionic liquids treated lignocelluloses. Efforts have been taken to improve the enzyme production through statistical optimization approach namely Plackett–Burman design and the Face Centered Central Composite Design (FCCCD). Plackett–Burman experimental design was used to screen the medium components and process conditions. It was found that carboxymethylcellulose (CMC), FeSO4Æ7H2O, NaCl, MgSO4Æ7H2O, peptone, agitation speed and inoculum size significantly influence the production of halophilic cellulase. On the other hand, KH2PO4, KOH, yeast extract and temperature had a negative effect on enzyme production. Further optimization through FCCCD revealed that the optimization approach improved halophilic cellulase production from 0.029 U/ml to 0.0625 U/ml, which was approximately 2.2-times greater than before optimization. Elsevier Ltd. 2015-07 Article REM application/pdf en http://irep.iium.edu.my/44276/1/Saudi_paper.pdf Nagoor Gunny, Ahmad Anas and Arbain, Dachyar and Jamal, Parveen and Gumba, Rizo Edwin (2015) Improvement of halophilic cellulase production from locally isolated fungal strain. Saudi Journal of Biological Sciences, 22 (4). pp. 476-483. http://www.sciencedirect.com/science/article/pii/S1319562X14001673# 10.1016/j.sjbs.2014.11.021
institution Universiti Islam Antarabangsa Malaysia
building IIUM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider International Islamic University Malaysia
content_source IIUM Repository (IREP)
url_provider http://irep.iium.edu.my/
language English
topic T Technology (General)
spellingShingle T Technology (General)
Nagoor Gunny, Ahmad Anas
Arbain, Dachyar
Jamal, Parveen
Gumba, Rizo Edwin
Improvement of halophilic cellulase production from locally isolated fungal strain
description Halophilic cellulases from the newly isolated fungus, Aspergillus terreus UniMAP AA-6 were found to be useful for in situ saccharification of ionic liquids treated lignocelluloses. Efforts have been taken to improve the enzyme production through statistical optimization approach namely Plackett–Burman design and the Face Centered Central Composite Design (FCCCD). Plackett–Burman experimental design was used to screen the medium components and process conditions. It was found that carboxymethylcellulose (CMC), FeSO4Æ7H2O, NaCl, MgSO4Æ7H2O, peptone, agitation speed and inoculum size significantly influence the production of halophilic cellulase. On the other hand, KH2PO4, KOH, yeast extract and temperature had a negative effect on enzyme production. Further optimization through FCCCD revealed that the optimization approach improved halophilic cellulase production from 0.029 U/ml to 0.0625 U/ml, which was approximately 2.2-times greater than before optimization.
format Article
author Nagoor Gunny, Ahmad Anas
Arbain, Dachyar
Jamal, Parveen
Gumba, Rizo Edwin
author_facet Nagoor Gunny, Ahmad Anas
Arbain, Dachyar
Jamal, Parveen
Gumba, Rizo Edwin
author_sort Nagoor Gunny, Ahmad Anas
title Improvement of halophilic cellulase production from locally isolated fungal strain
title_short Improvement of halophilic cellulase production from locally isolated fungal strain
title_full Improvement of halophilic cellulase production from locally isolated fungal strain
title_fullStr Improvement of halophilic cellulase production from locally isolated fungal strain
title_full_unstemmed Improvement of halophilic cellulase production from locally isolated fungal strain
title_sort improvement of halophilic cellulase production from locally isolated fungal strain
publisher Elsevier Ltd.
publishDate 2015
url http://irep.iium.edu.my/44276/1/Saudi_paper.pdf
http://irep.iium.edu.my/44276/
http://www.sciencedirect.com/science/article/pii/S1319562X14001673#
_version_ 1643612544750845952
score 13.211869

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