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Anti-proliferative activities of purified bacteriocin from enterococcus mundtii strain c4l10 isolated from the caecum of Malaysian non-broiler chicken on cancer cell lines

This study aim at experimenting the effects of the bacteriocin from Ent. mundtii strain C4L10, an apoptogenic properties, that have the ability to inhibit bacteria at the same time the tendency to induce anti-proliferative characteristics on cancer cell lines. A purified bacteriocin from Ent. mund...

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Main Authors: A Yusuf, Moshood, Arief Ichwan, Solachuddin Jauhari, Tengku Abdul Hamid, Tengku Haziyamin
Format: Article
Language:English
Published: Academic Sciences 2015
Subjects:
Q Science (General)
Online Access:http://irep.iium.edu.my/41183/1/3595-15384-1-PB.pdf
http://irep.iium.edu.my/41183/
http://innovareacademics.in/journals/index.php/ijpps/article/view/3595
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spelling my.iium.irep.411832016-02-04T08:54:15Z http://irep.iium.edu.my/41183/ Anti-proliferative activities of purified bacteriocin from enterococcus mundtii strain c4l10 isolated from the caecum of Malaysian non-broiler chicken on cancer cell lines A Yusuf, Moshood Arief Ichwan, Solachuddin Jauhari Tengku Abdul Hamid, Tengku Haziyamin Q Science (General) This study aim at experimenting the effects of the bacteriocin from Ent. mundtii strain C4L10, an apoptogenic properties, that have the ability to inhibit bacteria at the same time the tendency to induce anti-proliferative characteristics on cancer cell lines. A purified bacteriocin from Ent. mundtii C4L10 strain (accession number KC731423) isolated from Malaysian non-broiler chicken was screened for its anti-proliferative activities against four cancer cell lines; breast cancer (MCF7), lung cancer (H1299), colon cancer (HCT116) and oral cancer (HSC3) by an in vitro MTT [3-(4,5-dimethyl thiazol-2-yl)-2,5-diphenyl tetrazolium bromide], colorimetric assay. The half maximal inhibition, IC 50 values was determined by non-linear regression analysis using the Prism software (Graphpad). Results: The susceptibility of cancer cell lines to the bacteriocin extract was in the order: oral cancer HSC3, breast cancer MCF7, lung cancer, H1299 and colon cancer HCT116 with the IC50 values, 9.00 μg/ml, 11.51 μg/ml, 15.25 μg/ml, and 20.57 μg/ml, respectively. The approximate molecular weight for this purified bacteriocin was 10 kDa, as observed by SDS-PAGE. Bacteriocin from Ent mundtii strain C4L10 could be employed as potential antimicrobial agent and for anti-proliferative agent in cancer control. Academic Sciences 2015 Article REM application/pdf en http://irep.iium.edu.my/41183/1/3595-15384-1-PB.pdf A Yusuf, Moshood and Arief Ichwan, Solachuddin Jauhari and Tengku Abdul Hamid, Tengku Haziyamin (2015) Anti-proliferative activities of purified bacteriocin from enterococcus mundtii strain c4l10 isolated from the caecum of Malaysian non-broiler chicken on cancer cell lines. International Journal of Pharmacy and Pharmaceutical Sciences, 7 (2). pp. 334-337. ISSN 0975-1491 http://innovareacademics.in/journals/index.php/ijpps/article/view/3595
institution Universiti Islam Antarabangsa Malaysia
building IIUM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider International Islamic University Malaysia
content_source IIUM Repository (IREP)
url_provider http://irep.iium.edu.my/
language English
topic Q Science (General)
spellingShingle Q Science (General)
A Yusuf, Moshood
Arief Ichwan, Solachuddin Jauhari
Tengku Abdul Hamid, Tengku Haziyamin
Anti-proliferative activities of purified bacteriocin from enterococcus mundtii strain c4l10 isolated from the caecum of Malaysian non-broiler chicken on cancer cell lines
description This study aim at experimenting the effects of the bacteriocin from Ent. mundtii strain C4L10, an apoptogenic properties, that have the ability to inhibit bacteria at the same time the tendency to induce anti-proliferative characteristics on cancer cell lines. A purified bacteriocin from Ent. mundtii C4L10 strain (accession number KC731423) isolated from Malaysian non-broiler chicken was screened for its anti-proliferative activities against four cancer cell lines; breast cancer (MCF7), lung cancer (H1299), colon cancer (HCT116) and oral cancer (HSC3) by an in vitro MTT [3-(4,5-dimethyl thiazol-2-yl)-2,5-diphenyl tetrazolium bromide], colorimetric assay. The half maximal inhibition, IC 50 values was determined by non-linear regression analysis using the Prism software (Graphpad). Results: The susceptibility of cancer cell lines to the bacteriocin extract was in the order: oral cancer HSC3, breast cancer MCF7, lung cancer, H1299 and colon cancer HCT116 with the IC50 values, 9.00 μg/ml, 11.51 μg/ml, 15.25 μg/ml, and 20.57 μg/ml, respectively. The approximate molecular weight for this purified bacteriocin was 10 kDa, as observed by SDS-PAGE. Bacteriocin from Ent mundtii strain C4L10 could be employed as potential antimicrobial agent and for anti-proliferative agent in cancer control.
format Article
author A Yusuf, Moshood
Arief Ichwan, Solachuddin Jauhari
Tengku Abdul Hamid, Tengku Haziyamin
author_facet A Yusuf, Moshood
Arief Ichwan, Solachuddin Jauhari
Tengku Abdul Hamid, Tengku Haziyamin
author_sort A Yusuf, Moshood
title Anti-proliferative activities of purified bacteriocin from enterococcus mundtii strain c4l10 isolated from the caecum of Malaysian non-broiler chicken on cancer cell lines
title_short Anti-proliferative activities of purified bacteriocin from enterococcus mundtii strain c4l10 isolated from the caecum of Malaysian non-broiler chicken on cancer cell lines
title_full Anti-proliferative activities of purified bacteriocin from enterococcus mundtii strain c4l10 isolated from the caecum of Malaysian non-broiler chicken on cancer cell lines
title_fullStr Anti-proliferative activities of purified bacteriocin from enterococcus mundtii strain c4l10 isolated from the caecum of Malaysian non-broiler chicken on cancer cell lines
title_full_unstemmed Anti-proliferative activities of purified bacteriocin from enterococcus mundtii strain c4l10 isolated from the caecum of Malaysian non-broiler chicken on cancer cell lines
title_sort anti-proliferative activities of purified bacteriocin from enterococcus mundtii strain c4l10 isolated from the caecum of malaysian non-broiler chicken on cancer cell lines
publisher Academic Sciences
publishDate 2015
url http://irep.iium.edu.my/41183/1/3595-15384-1-PB.pdf
http://irep.iium.edu.my/41183/
http://innovareacademics.in/journals/index.php/ijpps/article/view/3595
_version_ 1643611968453476352
score 13.211869

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