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Neural-differentiated mesenchymal stem cells incorporated into muscle stuffed vein scaffold forms a stable living nerve conduit

Autologous nerve grafts to bridge nerve gaps have donor site morbidity and possible neuroma formation resulting in development of various methods of bridging nerve gaps without using autologous nerve grafts. We have fabricated an acellular muscle stuffed vein seeded with differentiated mesenchymal...

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Main Authors: Hassan, Nur Hidayah, Sulong, Ahmad Fadzli, Ng, Min-Hwei, Htwe, Ohnmar, H. Idrus, Ruszymah B., Roohi, Sharifah, Naicker, Amaramalar S., Abdullah, Shalimar
Format: Article
Language:English
Published: Orthopaedic Research Society 2012
Subjects:
RD701 Orthopedics
Online Access:http://irep.iium.edu.my/34420/1/journal_of_orthopaedic_research_2012.pdf
http://irep.iium.edu.my/34420/
http://onlinelibrary.wiley.com/journal/10.1002/%28ISSN%291554-527X/issues?year=2012
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spelling my.iium.irep.344202016-11-04T00:50:36Z http://irep.iium.edu.my/34420/ Neural-differentiated mesenchymal stem cells incorporated into muscle stuffed vein scaffold forms a stable living nerve conduit Hassan, Nur Hidayah Sulong, Ahmad Fadzli Ng, Min-Hwei Htwe, Ohnmar H. Idrus, Ruszymah B. Roohi, Sharifah Naicker, Amaramalar S. Abdullah, Shalimar RD701 Orthopedics Autologous nerve grafts to bridge nerve gaps have donor site morbidity and possible neuroma formation resulting in development of various methods of bridging nerve gaps without using autologous nerve grafts. We have fabricated an acellular muscle stuffed vein seeded with differentiated mesenchymal stem cells (MSCs) as a substitute for nerve autografts. Human vein and muscle were both decellularized by liquid nitrogen immersion with subsequent hydrolysis in hydrochloric acid. Human MSCs were subjected to a series of treatments with a reducing agent, retinoic acid, and a combination of trophic factors. The differentiated MSCs were seeded on the surface of acellular muscle tissue and then stuffed into the vein. Our study showed that 35–75% of the cells expressed neural markers such as S100b, glial fibrillary acidic protein (GFAP), p75 NGF receptor, and Nestin after differentiation. Histological and ultra structural analyses of muscle stuffed veins showed attachment of cells onto the surface of the acellular muscle and penetration of the cells into the hydrolyzed fraction of muscle fibers. We implanted these muscle stuffed veins into athymic mice and at 8 weeks post-implantation, the acellular muscle tissue had fully degraded and replaced with new matrix produced by the seeded cells. The vein was still intact and no inflammatory reactions were observed proving the biocompatibility and biodegradability of the conduit. In conclusion, we have successfully formed a stable living nerve conduit which may serve as a substitute for autologous nerves Orthopaedic Research Society 2012-10 Article REM application/pdf en http://irep.iium.edu.my/34420/1/journal_of_orthopaedic_research_2012.pdf Hassan, Nur Hidayah and Sulong, Ahmad Fadzli and Ng, Min-Hwei and Htwe, Ohnmar and H. Idrus, Ruszymah B. and Roohi, Sharifah and Naicker, Amaramalar S. and Abdullah, Shalimar (2012) Neural-differentiated mesenchymal stem cells incorporated into muscle stuffed vein scaffold forms a stable living nerve conduit. Journal of Orthopaedic Research , 30 (10). pp. 1674-1681. ISSN 0736-0266 http://onlinelibrary.wiley.com/journal/10.1002/%28ISSN%291554-527X/issues?year=2012
institution Universiti Islam Antarabangsa Malaysia
building IIUM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider International Islamic University Malaysia
content_source IIUM Repository (IREP)
url_provider http://irep.iium.edu.my/
language English
topic RD701 Orthopedics
spellingShingle RD701 Orthopedics
Hassan, Nur Hidayah
Sulong, Ahmad Fadzli
Ng, Min-Hwei
Htwe, Ohnmar
H. Idrus, Ruszymah B.
Roohi, Sharifah
Naicker, Amaramalar S.
Abdullah, Shalimar
Neural-differentiated mesenchymal stem cells incorporated into muscle stuffed vein scaffold forms a stable living nerve conduit
description Autologous nerve grafts to bridge nerve gaps have donor site morbidity and possible neuroma formation resulting in development of various methods of bridging nerve gaps without using autologous nerve grafts. We have fabricated an acellular muscle stuffed vein seeded with differentiated mesenchymal stem cells (MSCs) as a substitute for nerve autografts. Human vein and muscle were both decellularized by liquid nitrogen immersion with subsequent hydrolysis in hydrochloric acid. Human MSCs were subjected to a series of treatments with a reducing agent, retinoic acid, and a combination of trophic factors. The differentiated MSCs were seeded on the surface of acellular muscle tissue and then stuffed into the vein. Our study showed that 35–75% of the cells expressed neural markers such as S100b, glial fibrillary acidic protein (GFAP), p75 NGF receptor, and Nestin after differentiation. Histological and ultra structural analyses of muscle stuffed veins showed attachment of cells onto the surface of the acellular muscle and penetration of the cells into the hydrolyzed fraction of muscle fibers. We implanted these muscle stuffed veins into athymic mice and at 8 weeks post-implantation, the acellular muscle tissue had fully degraded and replaced with new matrix produced by the seeded cells. The vein was still intact and no inflammatory reactions were observed proving the biocompatibility and biodegradability of the conduit. In conclusion, we have successfully formed a stable living nerve conduit which may serve as a substitute for autologous nerves
format Article
author Hassan, Nur Hidayah
Sulong, Ahmad Fadzli
Ng, Min-Hwei
Htwe, Ohnmar
H. Idrus, Ruszymah B.
Roohi, Sharifah
Naicker, Amaramalar S.
Abdullah, Shalimar
author_facet Hassan, Nur Hidayah
Sulong, Ahmad Fadzli
Ng, Min-Hwei
Htwe, Ohnmar
H. Idrus, Ruszymah B.
Roohi, Sharifah
Naicker, Amaramalar S.
Abdullah, Shalimar
author_sort Hassan, Nur Hidayah
title Neural-differentiated mesenchymal stem cells incorporated into muscle stuffed vein scaffold forms a stable living nerve conduit
title_short Neural-differentiated mesenchymal stem cells incorporated into muscle stuffed vein scaffold forms a stable living nerve conduit
title_full Neural-differentiated mesenchymal stem cells incorporated into muscle stuffed vein scaffold forms a stable living nerve conduit
title_fullStr Neural-differentiated mesenchymal stem cells incorporated into muscle stuffed vein scaffold forms a stable living nerve conduit
title_full_unstemmed Neural-differentiated mesenchymal stem cells incorporated into muscle stuffed vein scaffold forms a stable living nerve conduit
title_sort neural-differentiated mesenchymal stem cells incorporated into muscle stuffed vein scaffold forms a stable living nerve conduit
publisher Orthopaedic Research Society
publishDate 2012
url http://irep.iium.edu.my/34420/1/journal_of_orthopaedic_research_2012.pdf
http://irep.iium.edu.my/34420/
http://onlinelibrary.wiley.com/journal/10.1002/%28ISSN%291554-527X/issues?year=2012
_version_ 1643610622988910592
score 13.211869

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