Fabrication of micropillar sheet for cell culture dish
Cell culture is one of the major tools in life sciences. Conventionally, cells are placed in a flat petri dish made of glass or plastic. It is used to monitor cells behavior under controlled conditions. As the cells are strongly adhered to petri dish, an additional process of trypsinization is appl...
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| Main Authors: | , |
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| Format: | Conference or Workshop Item |
| Language: | English |
| Published: |
2011
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| Subjects: | |
| Online Access: | http://irep.iium.edu.my/28907/1/082_MTE_2011_Poster_Fabrication_of_Micro.pdf http://irep.iium.edu.my/28907/ |
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| Summary: | Cell culture is one of the major tools in life sciences. Conventionally, cells are placed in a flat petri dish made of glass or plastic. It is used to
monitor cells behavior under controlled conditions. As the cells are strongly adhered to petri dish, an additional process of trypsinization is applied
to remove the cells from the dish. Alternatively, by using micropillar sheet, the contact surface between the cells and the pillars is smaller than
traditional two dimensional petri dish. Cells were found to grow in a different way on the micropillars because of these differences in adherence
to the support materials. The micropillars structure enables the cells to be removed without any additional process where the cultured cells can
be collected simply by pipetting. However, it remains a challenge to fabricate high aspect ratio metallic or polymeric micropillar sheets of about 1
μm or less of the micropillar diameters. This research presents the fabrication process using focused ion beam (FIB) a maskless sputtering
technique for the fabrication of micropillar sheet to be used in cell culture dish. Micropore sheet on metallic materials is also machined using FIB
sputtering which can be used as a master microtool for the mass production of polymer micropillar sheets by micro/nano hot embossing. The
typical diameter of the micropillars is 1 μm with 3-10 aspect ratio. This FIB based process is direct, easy to fabricate, and less expensive
compared to LIGA (lithography, electroplating and molding) and lithography based techniques as reported. |
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