In-vitro evaluation of antioxidant activity of Phaleria macrocarpa using different extraction process and determination of functional groups in the extract by FTIR (Fourier Transform Infrared Spectroscopy)
P. macrocarpa is often known as “God’s crown” due to its great nutritional importance for human health. P. macrocarpa fruit can delay and avoid oxidative cell damage in the body by its antioxidant properties. In the meantime, there is still lacking yield, antioxidant activity, and functional propert...
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Malaysian Institute of Chemistry
2024
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my.iium.irep.1163702024-12-05T04:20:55Z http://irep.iium.edu.my/116370/ In-vitro evaluation of antioxidant activity of Phaleria macrocarpa using different extraction process and determination of functional groups in the extract by FTIR (Fourier Transform Infrared Spectroscopy) Anisya, Shifa Nur Nisa, Fithri Choirun Liew, Kai Bin Helal Uddin, A.B.M. QD Chemistry RM Therapeutics. Pharmacology RS Pharmacy and materia medica RS192 Materia Medica-Pharmaceutical Technology RS403 Materia Medica-Pharmaceutical Chemistry P. macrocarpa is often known as “God’s crown” due to its great nutritional importance for human health. P. macrocarpa fruit can delay and avoid oxidative cell damage in the body by its antioxidant properties. In the meantime, there is still lacking yield, antioxidant activity, and functional properties of P. macrocarpa fruit in the sonication method using water and ethanol. So, this study aims to compare P. macrocarpa fruit yield, and antioxidant properties and to identify its functional group. Crude ethanol extract and water extract of P. macrocarpa fruit have been studied for Ferric Reducing Antioxidant Power (FRAP) and 2,2- Diphenyl-1- Picrylhydrazyl (DPPH) antioxidant activities against Ascorbic acid as a standard. The study also determined the functional group using FTIR (Fourier Transform Infrared Spectroscopy) assay. The most effective P. macrocarpa fruit yield was obtained in the water extract compared to the ethanol extract. In addition, the ethanol extract showed a higher FRAP (54.285±1.139 mgAAE/g) effect than the water extract. Besides, the DPPH activity of ethanol extract (46.113±1.535 µg/mL) was higher than water extract (59.406±2.089 µg/mL) and lower than standard Ascorbic acid (8.287±0.125 µg/mL). Moreover, some functional groups such as -OH, aliphatic CH, aromatic CH, aromatic C=C and C=N were also identified in both P. macrocarpa fruit extracts. The results confirmed that the sonication method using ethanol and water could influence the yield of P. macrocarpa fruit extracts as well as antioxidant activities with different functional groups. Malaysian Institute of Chemistry 2024-10-21 Article PeerReviewed application/pdf en http://irep.iium.edu.my/116370/31/116370_In-vitro%20evaluation%20of%20antioxidant%20activity%20of%20Phaleria%20macrocarpa.pdf application/pdf en http://irep.iium.edu.my/116370/32/116370_In-vitro%20evaluation%20of%20antioxidant%20activity%20of%20Phaleria%20macrocarpa_Scopus.pdf Anisya, Shifa Nur and Nisa, Fithri Choirun and Liew, Kai Bin and Helal Uddin, A.B.M. (2024) In-vitro evaluation of antioxidant activity of Phaleria macrocarpa using different extraction process and determination of functional groups in the extract by FTIR (Fourier Transform Infrared Spectroscopy). Malaysian Journal of Chemistry, 26 (5). pp. 424-430. ISSN 1511-2292 E-ISSN 2550-1658 https://ikm.org.my/publications/malaysian-journal-of-chemistry/view-abstract.php?abs=J0052-cee5da4 10.55373/mjchem.v26i5.424 |
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QD Chemistry RM Therapeutics. Pharmacology RS Pharmacy and materia medica RS192 Materia Medica-Pharmaceutical Technology RS403 Materia Medica-Pharmaceutical Chemistry Anisya, Shifa Nur Nisa, Fithri Choirun Liew, Kai Bin Helal Uddin, A.B.M. In-vitro evaluation of antioxidant activity of Phaleria macrocarpa using different extraction process and determination of functional groups in the extract by FTIR (Fourier Transform Infrared Spectroscopy) |
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P. macrocarpa is often known as “God’s crown” due to its great nutritional importance for human health. P. macrocarpa fruit can delay and avoid oxidative cell damage in the body by its antioxidant properties. In the meantime, there is still lacking yield, antioxidant activity, and functional properties of P. macrocarpa fruit in the sonication method using water and ethanol. So, this study aims to compare P. macrocarpa fruit yield, and antioxidant properties and to identify its functional group. Crude ethanol extract and water extract of P. macrocarpa fruit have been studied for Ferric Reducing Antioxidant Power (FRAP) and 2,2- Diphenyl-1- Picrylhydrazyl (DPPH) antioxidant activities against Ascorbic acid as a standard. The study also determined the functional group using FTIR (Fourier Transform Infrared Spectroscopy) assay. The most effective P. macrocarpa fruit yield was obtained in the water extract compared to the ethanol extract. In addition, the ethanol extract showed a higher FRAP (54.285±1.139 mgAAE/g) effect than the water extract. Besides, the DPPH activity of ethanol extract (46.113±1.535 µg/mL) was higher than water extract (59.406±2.089 µg/mL) and lower than standard Ascorbic acid (8.287±0.125 µg/mL). Moreover, some functional groups such as -OH, aliphatic CH, aromatic CH, aromatic C=C and C=N were also identified in both P. macrocarpa fruit extracts. The results confirmed that the sonication method using ethanol and water could influence the yield of P. macrocarpa fruit extracts as well as antioxidant activities with different functional groups. |
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Article |
author |
Anisya, Shifa Nur Nisa, Fithri Choirun Liew, Kai Bin Helal Uddin, A.B.M. |
author_facet |
Anisya, Shifa Nur Nisa, Fithri Choirun Liew, Kai Bin Helal Uddin, A.B.M. |
author_sort |
Anisya, Shifa Nur |
title |
In-vitro evaluation of antioxidant activity of Phaleria macrocarpa using different extraction process and determination of functional groups in the extract by FTIR (Fourier Transform Infrared Spectroscopy) |
title_short |
In-vitro evaluation of antioxidant activity of Phaleria macrocarpa using different extraction process and determination of functional groups in the extract by FTIR (Fourier Transform Infrared Spectroscopy) |
title_full |
In-vitro evaluation of antioxidant activity of Phaleria macrocarpa using different extraction process and determination of functional groups in the extract by FTIR (Fourier Transform Infrared Spectroscopy) |
title_fullStr |
In-vitro evaluation of antioxidant activity of Phaleria macrocarpa using different extraction process and determination of functional groups in the extract by FTIR (Fourier Transform Infrared Spectroscopy) |
title_full_unstemmed |
In-vitro evaluation of antioxidant activity of Phaleria macrocarpa using different extraction process and determination of functional groups in the extract by FTIR (Fourier Transform Infrared Spectroscopy) |
title_sort |
in-vitro evaluation of antioxidant activity of phaleria macrocarpa using different extraction process and determination of functional groups in the extract by ftir (fourier transform infrared spectroscopy) |
publisher |
Malaysian Institute of Chemistry |
publishDate |
2024 |
url |
http://irep.iium.edu.my/116370/31/116370_In-vitro%20evaluation%20of%20antioxidant%20activity%20of%20Phaleria%20macrocarpa.pdf http://irep.iium.edu.my/116370/32/116370_In-vitro%20evaluation%20of%20antioxidant%20activity%20of%20Phaleria%20macrocarpa_Scopus.pdf http://irep.iium.edu.my/116370/ https://ikm.org.my/publications/malaysian-journal-of-chemistry/view-abstract.php?abs=J0052-cee5da4 |
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13.222552 |