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Novel Rhizophus oryzae esterase-immobilized chitosan/carbon nanomaterials as sensing support for enzyme inhibition-based biosensor in the detection of organophosphorus pesticide residues on crops

The use of mobile biosensor would allow for cost-effective and rapid on-site detection of organophosphorus (OP) pesticide. Immobilizing enzyme on electrode would improve the sensitivity of biosensor due to the nature of its specific active site-binding. OP inhibition on esterase enzyme is used as th...

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Main Authors: Mohd Fadzely, Amir Hafiz, Ahmad, Farah, Mat Harttar @ Mohd Hatta, Maziati Akmal
Format: Article
Language:English
Published: Elsevier 2024
Subjects:
QD Chemistry
QH301 Biology
TD Environmental technology. Sanitary engineering
TK Electrical engineering. Electronics Nuclear engineering
TP Chemical technology
Online Access:http://irep.iium.edu.my/113357/1/113357_Novel%20Rhizophus%20oryzae%20esterase.pdf
http://irep.iium.edu.my/113357/
https://www.sciencedirect.com/science/article/pii/S277307862400027X?via%3Dihub
https://doi.org/10.1016/j.napere.2024.100092
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spelling my.iium.irep.1133572024-08-02T02:01:36Z http://irep.iium.edu.my/113357/ Novel Rhizophus oryzae esterase-immobilized chitosan/carbon nanomaterials as sensing support for enzyme inhibition-based biosensor in the detection of organophosphorus pesticide residues on crops Mohd Fadzely, Amir Hafiz Ahmad, Farah Mat Harttar @ Mohd Hatta, Maziati Akmal QD Chemistry QH301 Biology TD Environmental technology. Sanitary engineering TK Electrical engineering. Electronics Nuclear engineering TP Chemical technology The use of mobile biosensor would allow for cost-effective and rapid on-site detection of organophosphorus (OP) pesticide. Immobilizing enzyme on electrode would improve the sensitivity of biosensor due to the nature of its specific active site-binding. OP inhibition on esterase enzyme is used as the sensing mechanism of the biosensor. In this study, microbial esterase from Rhizopus oryzae (RoE) was immobilized into modified screen-printed carbon electrode (SPCE) for the fabrication of novel RoE/chitosan/nanomaterial-COOH/SPCE biosensor for the detection of methyl parathion, an OP pesticide. The first phase of this study aims to analyze biochemical characteristics of 1-naphthyl acetate (1-NA) hydrolysis by RoE and enzymatic inhibition of RoE by OP pesticide. RoE kinetic showed the best fit with Langmuir model with the highest linear regression (R2 = 0.996). The inhibition of RoE by OP was shown to be linearly proportional to the enzyme activity (R2 = 0.827). The second phase of this study aims to analyze electrochemical performance of RoE-immobilized biosensor. Various carbon-based sensing support nanomaterials (graphene nanoplatelets (GNP), graphite and multi-walled carbon nanotube (MWCNT)) was functionalised by carboxyl (-COOH) group and screened, where all nanomaterials showed effective sensitivity towards detecting 0.01 ng/L methyl parathion pesticide. GNP-based biosensor exhibited the highest degree of inhibition (9.94 %) and better reproducibility after the first cycle. RoE/chitosan/GNP-COOH/SPCE biosensor is promising to be developed as cost-effective and highly sensitive biosensor for OP pesticide detection. Elsevier 2024-07-22 Article PeerReviewed application/pdf en http://irep.iium.edu.my/113357/1/113357_Novel%20Rhizophus%20oryzae%20esterase.pdf Mohd Fadzely, Amir Hafiz and Ahmad, Farah and Mat Harttar @ Mohd Hatta, Maziati Akmal (2024) Novel Rhizophus oryzae esterase-immobilized chitosan/carbon nanomaterials as sensing support for enzyme inhibition-based biosensor in the detection of organophosphorus pesticide residues on crops. Journal of Natural Pesticide Research, 10. pp. 1-13. E-ISSN 2773-0786 https://www.sciencedirect.com/science/article/pii/S277307862400027X?via%3Dihub https://doi.org/10.1016/j.napere.2024.100092
institution Universiti Islam Antarabangsa Malaysia
building IIUM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider International Islamic University Malaysia
content_source IIUM Repository (IREP)
url_provider http://irep.iium.edu.my/
language English
topic QD Chemistry
QH301 Biology
TD Environmental technology. Sanitary engineering
TK Electrical engineering. Electronics Nuclear engineering
TP Chemical technology
spellingShingle QD Chemistry
QH301 Biology
TD Environmental technology. Sanitary engineering
TK Electrical engineering. Electronics Nuclear engineering
TP Chemical technology
Mohd Fadzely, Amir Hafiz
Ahmad, Farah
Mat Harttar @ Mohd Hatta, Maziati Akmal
Novel Rhizophus oryzae esterase-immobilized chitosan/carbon nanomaterials as sensing support for enzyme inhibition-based biosensor in the detection of organophosphorus pesticide residues on crops
description The use of mobile biosensor would allow for cost-effective and rapid on-site detection of organophosphorus (OP) pesticide. Immobilizing enzyme on electrode would improve the sensitivity of biosensor due to the nature of its specific active site-binding. OP inhibition on esterase enzyme is used as the sensing mechanism of the biosensor. In this study, microbial esterase from Rhizopus oryzae (RoE) was immobilized into modified screen-printed carbon electrode (SPCE) for the fabrication of novel RoE/chitosan/nanomaterial-COOH/SPCE biosensor for the detection of methyl parathion, an OP pesticide. The first phase of this study aims to analyze biochemical characteristics of 1-naphthyl acetate (1-NA) hydrolysis by RoE and enzymatic inhibition of RoE by OP pesticide. RoE kinetic showed the best fit with Langmuir model with the highest linear regression (R2 = 0.996). The inhibition of RoE by OP was shown to be linearly proportional to the enzyme activity (R2 = 0.827). The second phase of this study aims to analyze electrochemical performance of RoE-immobilized biosensor. Various carbon-based sensing support nanomaterials (graphene nanoplatelets (GNP), graphite and multi-walled carbon nanotube (MWCNT)) was functionalised by carboxyl (-COOH) group and screened, where all nanomaterials showed effective sensitivity towards detecting 0.01 ng/L methyl parathion pesticide. GNP-based biosensor exhibited the highest degree of inhibition (9.94 %) and better reproducibility after the first cycle. RoE/chitosan/GNP-COOH/SPCE biosensor is promising to be developed as cost-effective and highly sensitive biosensor for OP pesticide detection.
format Article
author Mohd Fadzely, Amir Hafiz
Ahmad, Farah
Mat Harttar @ Mohd Hatta, Maziati Akmal
author_facet Mohd Fadzely, Amir Hafiz
Ahmad, Farah
Mat Harttar @ Mohd Hatta, Maziati Akmal
author_sort Mohd Fadzely, Amir Hafiz
title Novel Rhizophus oryzae esterase-immobilized chitosan/carbon nanomaterials as sensing support for enzyme inhibition-based biosensor in the detection of organophosphorus pesticide residues on crops
title_short Novel Rhizophus oryzae esterase-immobilized chitosan/carbon nanomaterials as sensing support for enzyme inhibition-based biosensor in the detection of organophosphorus pesticide residues on crops
title_full Novel Rhizophus oryzae esterase-immobilized chitosan/carbon nanomaterials as sensing support for enzyme inhibition-based biosensor in the detection of organophosphorus pesticide residues on crops
title_fullStr Novel Rhizophus oryzae esterase-immobilized chitosan/carbon nanomaterials as sensing support for enzyme inhibition-based biosensor in the detection of organophosphorus pesticide residues on crops
title_full_unstemmed Novel Rhizophus oryzae esterase-immobilized chitosan/carbon nanomaterials as sensing support for enzyme inhibition-based biosensor in the detection of organophosphorus pesticide residues on crops
title_sort novel rhizophus oryzae esterase-immobilized chitosan/carbon nanomaterials as sensing support for enzyme inhibition-based biosensor in the detection of organophosphorus pesticide residues on crops
publisher Elsevier
publishDate 2024
url http://irep.iium.edu.my/113357/1/113357_Novel%20Rhizophus%20oryzae%20esterase.pdf
http://irep.iium.edu.my/113357/
https://www.sciencedirect.com/science/article/pii/S277307862400027X?via%3Dihub
https://doi.org/10.1016/j.napere.2024.100092
_version_ 1806442136396103680
score 13.211869

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