Novel Rhizophus oryzae esterase-immobilized chitosan/carbon nanomaterials as sensing support for enzyme inhibition-based biosensor in the detection of organophosphorus pesticide residues on crops
The use of mobile biosensor would allow for cost-effective and rapid on-site detection of organophosphorus (OP) pesticide. Immobilizing enzyme on electrode would improve the sensitivity of biosensor due to the nature of its specific active site-binding. OP inhibition on esterase enzyme is used as th...
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| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2024
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| Subjects: | |
| Online Access: | http://irep.iium.edu.my/113357/1/113357_Novel%20Rhizophus%20oryzae%20esterase.pdf http://irep.iium.edu.my/113357/ https://www.sciencedirect.com/science/article/pii/S277307862400027X?via%3Dihub https://doi.org/10.1016/j.napere.2024.100092 |
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| Summary: | The use of mobile biosensor would allow for cost-effective and rapid on-site detection of organophosphorus (OP) pesticide. Immobilizing enzyme on electrode would improve the sensitivity of biosensor due to the nature of its specific active site-binding. OP inhibition on esterase enzyme is used as the sensing mechanism of the biosensor. In this study, microbial esterase from Rhizopus oryzae (RoE) was immobilized into modified screen-printed carbon electrode (SPCE) for the fabrication of novel RoE/chitosan/nanomaterial-COOH/SPCE biosensor for the detection of methyl parathion, an OP pesticide. The first phase of this study aims to analyze biochemical characteristics of 1-naphthyl acetate (1-NA) hydrolysis by RoE and enzymatic inhibition of RoE by OP pesticide. RoE kinetic showed the best fit with Langmuir model with the highest linear regression (R2 = 0.996). The inhibition of RoE by OP was shown to be linearly proportional to the enzyme activity (R2 = 0.827). The second phase of this study aims to analyze electrochemical performance of RoE-immobilized biosensor. Various carbon-based sensing support nanomaterials (graphene nanoplatelets (GNP), graphite and multi-walled carbon nanotube (MWCNT)) was functionalised by carboxyl (-COOH) group and screened, where all nanomaterials showed effective sensitivity towards detecting 0.01 ng/L methyl parathion pesticide. GNP-based biosensor exhibited the highest degree of inhibition (9.94 %) and better reproducibility after the first cycle. RoE/chitosan/GNP-COOH/SPCE biosensor is promising to be developed as cost-effective and highly sensitive biosensor for OP pesticide detection. |
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