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Biomarker identification from urine of dengue patients through GCMS for initial development of non-invasive diagnostic kit

Dengue infection detection methods, namely dengue serology test and RealTime PCR, are only available in clinical laboratories or healthcare facilities. This is timeconsuming and inconvenient for patients. Thus, a non-invasive on-site urinary diagnostic kit for dengue infection that requires no trai...

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Main Authors: Amid, Azura, Abdullah Sani, Muhamad Shirwan, Abdullah, Aszrin, Mohamed Bakrim, Norbaiyah, Wan Omar, Wan Fatein Nabeila, Zakaria, Nurul Aulia, Abdullah, Nor Zamzila, Nik Ahmad, Nik Nur Fatnoon
Format: Article
Language:English
English
Published: IIUM Press 2024
Subjects:
QD Chemistry
Online Access:http://irep.iium.edu.my/113303/13/113303_Biomarker%20identification%20from%20urine.pdf
http://irep.iium.edu.my/113303/19/113303_Biomarker%20identification%20from%20urine_Scopus.pdf
http://irep.iium.edu.my/113303/
https://journals.iium.edu.my/ejournal/index.php/iiumej/article/view/2785
https://doi.org/10.31436/iiumej.v25i2.2785
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spelling my.iium.irep.1133032024-08-07T08:29:16Z http://irep.iium.edu.my/113303/ Biomarker identification from urine of dengue patients through GCMS for initial development of non-invasive diagnostic kit Amid, Azura Abdullah Sani, Muhamad Shirwan Abdullah, Aszrin Mohamed Bakrim, Norbaiyah Wan Omar, Wan Fatein Nabeila Zakaria, Nurul Aulia Abdullah, Nor Zamzila Nik Ahmad, Nik Nur Fatnoon QD Chemistry Dengue infection detection methods, namely dengue serology test and RealTime PCR, are only available in clinical laboratories or healthcare facilities. This is timeconsuming and inconvenient for patients. Thus, a non-invasive on-site urinary diagnostic kit for dengue infection that requires no trained personnel for blood extraction would be advantageous and warranted, albeit in developed or underdeveloped nations with limited resources. To develop the on-site diagnostic kit, an identification of biomarkers related to dengue infection is needed. This preliminary study aims to identify potential metabolite biomarkers from the urine of dengue patients using GCMS analysis. Urine samples of patients with serologically confirmed dengue infection were analyzed and compared with healthy volunteers. The study shows that the GCMS approach can identify differences in the urine of dengue patients from healthy volunteers. Heptacosane, Hexadecane, 2,4- bis(1,1dimethylethyl) phenol, 2-bromooctane, tetradecane, hexyl octyl ester sulfurous acid, 2benzoyl methyl ester benzoic acid, 2,9-dimethyl decane, and pentadecane were identified from the urine of dengue patients. The identified secretion of alkane may be a suitable candidate for colorimetric assay for the development of a user-friendly, home-screening rapid test kit for the detection of dengue infection. IIUM Press 2024-07-15 Article PeerReviewed application/pdf en http://irep.iium.edu.my/113303/13/113303_Biomarker%20identification%20from%20urine.pdf application/pdf en http://irep.iium.edu.my/113303/19/113303_Biomarker%20identification%20from%20urine_Scopus.pdf Amid, Azura and Abdullah Sani, Muhamad Shirwan and Abdullah, Aszrin and Mohamed Bakrim, Norbaiyah and Wan Omar, Wan Fatein Nabeila and Zakaria, Nurul Aulia and Abdullah, Nor Zamzila and Nik Ahmad, Nik Nur Fatnoon (2024) Biomarker identification from urine of dengue patients through GCMS for initial development of non-invasive diagnostic kit. IIUM Engineering Journal, 25 (2). pp. 1-16. ISSN 1511-788X E-ISSN 2289-7860 https://journals.iium.edu.my/ejournal/index.php/iiumej/article/view/2785 https://doi.org/10.31436/iiumej.v25i2.2785
institution Universiti Islam Antarabangsa Malaysia
building IIUM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider International Islamic University Malaysia
content_source IIUM Repository (IREP)
url_provider http://irep.iium.edu.my/
language English
English
topic QD Chemistry
spellingShingle QD Chemistry
Amid, Azura
Abdullah Sani, Muhamad Shirwan
Abdullah, Aszrin
Mohamed Bakrim, Norbaiyah
Wan Omar, Wan Fatein Nabeila
Zakaria, Nurul Aulia
Abdullah, Nor Zamzila
Nik Ahmad, Nik Nur Fatnoon
Biomarker identification from urine of dengue patients through GCMS for initial development of non-invasive diagnostic kit
description Dengue infection detection methods, namely dengue serology test and RealTime PCR, are only available in clinical laboratories or healthcare facilities. This is timeconsuming and inconvenient for patients. Thus, a non-invasive on-site urinary diagnostic kit for dengue infection that requires no trained personnel for blood extraction would be advantageous and warranted, albeit in developed or underdeveloped nations with limited resources. To develop the on-site diagnostic kit, an identification of biomarkers related to dengue infection is needed. This preliminary study aims to identify potential metabolite biomarkers from the urine of dengue patients using GCMS analysis. Urine samples of patients with serologically confirmed dengue infection were analyzed and compared with healthy volunteers. The study shows that the GCMS approach can identify differences in the urine of dengue patients from healthy volunteers. Heptacosane, Hexadecane, 2,4- bis(1,1dimethylethyl) phenol, 2-bromooctane, tetradecane, hexyl octyl ester sulfurous acid, 2benzoyl methyl ester benzoic acid, 2,9-dimethyl decane, and pentadecane were identified from the urine of dengue patients. The identified secretion of alkane may be a suitable candidate for colorimetric assay for the development of a user-friendly, home-screening rapid test kit for the detection of dengue infection.
format Article
author Amid, Azura
Abdullah Sani, Muhamad Shirwan
Abdullah, Aszrin
Mohamed Bakrim, Norbaiyah
Wan Omar, Wan Fatein Nabeila
Zakaria, Nurul Aulia
Abdullah, Nor Zamzila
Nik Ahmad, Nik Nur Fatnoon
author_facet Amid, Azura
Abdullah Sani, Muhamad Shirwan
Abdullah, Aszrin
Mohamed Bakrim, Norbaiyah
Wan Omar, Wan Fatein Nabeila
Zakaria, Nurul Aulia
Abdullah, Nor Zamzila
Nik Ahmad, Nik Nur Fatnoon
author_sort Amid, Azura
title Biomarker identification from urine of dengue patients through GCMS for initial development of non-invasive diagnostic kit
title_short Biomarker identification from urine of dengue patients through GCMS for initial development of non-invasive diagnostic kit
title_full Biomarker identification from urine of dengue patients through GCMS for initial development of non-invasive diagnostic kit
title_fullStr Biomarker identification from urine of dengue patients through GCMS for initial development of non-invasive diagnostic kit
title_full_unstemmed Biomarker identification from urine of dengue patients through GCMS for initial development of non-invasive diagnostic kit
title_sort biomarker identification from urine of dengue patients through gcms for initial development of non-invasive diagnostic kit
publisher IIUM Press
publishDate 2024
url http://irep.iium.edu.my/113303/13/113303_Biomarker%20identification%20from%20urine.pdf
http://irep.iium.edu.my/113303/19/113303_Biomarker%20identification%20from%20urine_Scopus.pdf
http://irep.iium.edu.my/113303/
https://journals.iium.edu.my/ejournal/index.php/iiumej/article/view/2785
https://doi.org/10.31436/iiumej.v25i2.2785
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score 13.211869

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