Biomarker identification from urine of dengue patients through GCMS for initial development of non-invasive diagnostic kit
Dengue infection detection methods, namely dengue serology test and RealTime PCR, are only available in clinical laboratories or healthcare facilities. This is timeconsuming and inconvenient for patients. Thus, a non-invasive on-site urinary diagnostic kit for dengue infection that requires no trai...
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| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English English |
| Published: |
IIUM Press
2024
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| Subjects: | |
| Online Access: | http://irep.iium.edu.my/113303/13/113303_Biomarker%20identification%20from%20urine.pdf http://irep.iium.edu.my/113303/19/113303_Biomarker%20identification%20from%20urine_Scopus.pdf http://irep.iium.edu.my/113303/ https://journals.iium.edu.my/ejournal/index.php/iiumej/article/view/2785 https://doi.org/10.31436/iiumej.v25i2.2785 |
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| Summary: | Dengue infection detection methods, namely dengue serology test and RealTime PCR, are only available in clinical laboratories or healthcare facilities. This is timeconsuming and inconvenient for patients. Thus, a non-invasive on-site urinary diagnostic kit
for dengue infection that requires no trained personnel for blood extraction would be
advantageous and warranted, albeit in developed or underdeveloped nations with limited
resources. To develop the on-site diagnostic kit, an identification of biomarkers related to
dengue infection is needed. This preliminary study aims to identify potential metabolite
biomarkers from the urine of dengue patients using GCMS analysis. Urine samples of patients
with serologically confirmed dengue infection were analyzed and compared with healthy
volunteers. The study shows that the GCMS approach can identify differences in the urine of
dengue patients from healthy volunteers. Heptacosane, Hexadecane, 2,4-
bis(1,1dimethylethyl) phenol, 2-bromooctane, tetradecane, hexyl octyl ester sulfurous acid,
2benzoyl methyl ester benzoic acid, 2,9-dimethyl decane, and pentadecane were identified
from the urine of dengue patients. The identified secretion of alkane may be a suitable
candidate for colorimetric assay for the development of a user-friendly, home-screening rapid
test kit for the detection of dengue infection. |
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