Staff View: Cytotoxicity study, antioxidant and antibacterial activity against plant pathogenic bacteria of methanol extract from Coscinium fenestratum (akar sekunyit) = Kajian ketoksikan sel, aktiviti antioksida dan sifat antibakteria ekstrak metanol dari Coscinium fenestratum (akar sekunyit) terhadap bakteria penyebab penyakit pokok

Cytotoxicity study, antioxidant and antibacterial activity against plant pathogenic bacteria of methanol extract from Coscinium fenestratum (akar sekunyit) = Kajian ketoksikan sel, aktiviti antioksida dan sifat antibakteria ekstrak metanol dari Coscinium fenestratum (akar sekunyit) terhadap bakteria penyebab penyakit pokok

Coscinium fenestratum (Gaertn.) Colebr. (Menispermaceae) is a large woody liana found in moist deciduous to the evergreen forest at an altitude of 350–1200m. The stem and root of this species are highly medicinal valuable, and it is believed to be an endangered species in Malaysia. This study was co...

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Main Authors:	Harun, Aiza, Abdul Aziz, Noorshilawati, Daud, Shaari, Salim, Khairil Syazwan, Abu Bakar, Norlaili, Mat So'Ad, Siti Zaiton, Ahmat, Norizan
Format:	Article
Language:	English English
Published:	Malaysian Analytical Sciences Society (ANALIS) 2023
Subjects:	QD Chemistry
Online Access:	http://irep.iium.edu.my/111484/1/111484_Cytotoxicity%20study%2C%20antioxidant%20and%20antibacterial%20activity.pdf http://irep.iium.edu.my/111484/2/111484_Cytotoxicity%20study%2C%20antioxidant%20and%20antibacterial%20activity_SCOPUS.pdf http://irep.iium.edu.my/111484/ https://mjas.analis.com.my/mjas/v27_n6/v27_n6.html
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id	my.iium.irep.111484
record_format	dspace
institution	Universiti Islam Antarabangsa Malaysia
building	IIUM Library
collection	Institutional Repository
continent	Asia
country	Malaysia
content_provider	International Islamic University Malaysia
content_source	IIUM Repository (IREP)
url_provider	http://irep.iium.edu.my/
language	English English
topic	QD Chemistry
spellingShingle	QD Chemistry Harun, Aiza Abdul Aziz, Noorshilawati Daud, Shaari Salim, Khairil Syazwan Abu Bakar, Norlaili Mat So'Ad, Siti Zaiton Ahmat, Norizan Cytotoxicity study, antioxidant and antibacterial activity against plant pathogenic bacteria of methanol extract from Coscinium fenestratum (akar sekunyit) = Kajian ketoksikan sel, aktiviti antioksida dan sifat antibakteria ekstrak metanol dari Coscinium fenestratum (akar sekunyit) terhadap bakteria penyebab penyakit pokok
description	Coscinium fenestratum (Gaertn.) Colebr. (Menispermaceae) is a large woody liana found in moist deciduous to the evergreen forest at an altitude of 350–1200m. The stem and root of this species are highly medicinal valuable, and it is believed to be an endangered species in Malaysia. This study was conducted to investigate the reaction of methanol extract from Coscinium fenestratum with cancer cell lines, plant pathogenic bacteria, as well as DPPH scavenging and ferric reduction antioxidant power. The antibacterial activity was evaluated from disc diffusion method and microdilution method against soft rot bacteria Erwinia chrysanthemi and Erwinia carotovora. The cytotoxicity assay was performed using MCF-7 and Caco-2 cancer cell lines. The antioxidant properties of methanol extract were assessed from DPPH radical scavenging activity and ferric reducing antioxidant power (FRAP). The cytotoxicity activity revealed the methanol extract has potential to suppress the Caco-2 cell line with the percentage cell viability at 70% compared to MCF-7 cell line. The antioxidant activity from FRAP assay recorded its value of 47.8080 µg for TE/gDW and 13.8481 µg/mL for the IC50 from radical scavenging activity. However, the total flavonoid content (TFC) was not detected in methanol extract of C. fenestratum. The antibacterial assay revealed that the extract had moderately inhibited the growth of E. chrysanthemi and E. carotovora at 400 mg/mL, with inhibition zones of 11.50 mm and 11.83 mm, respectively. Therefore, the methanol extract from C. fenestratum has potential to act as antioxidant and anti-soft rot agent but less potential as anti-cancer agent and anti-soft rot agent. ***** Coscinium fenestratum (Gaertn.) Colebr. (Menispermaceae), pokok liana berkayu besar yang ditemui dalam hutan daun luruh lembap hingga ke hutan malar hijau pada ketinggian 350–1200 m. Batang dan akar spesis ini sangat bernilai dalam bidang perubatan, dan ia dipercayai spesis terancam di Malaysia. Kajian ini bertujuan untuk mengkaji tindakan ekstrak metanol daripada Coscinium fenestratum bertindak balas dengan garisan sel kanser, bakteria patogen tumbuhan serta penghapusan DPPH dan kuasa antioksidan penurunan ferik (FRAP). Aktiviti antibakteria dinilai daripada kaedah resapan cakera dan kaedah pencairan mikro terhadap bakteria reput lembut Erwinia chrysanthemi dan Erwinia carotovora. Ujian ketoksikan sel telah diadakan menggunakan garisan sel kanser MCF-7 dan Caco-2. Sifat antioksidan ekstrak metanol dinilai daripada aktiviti penghapusan radikal DPPH dan kuasa antioksidan penurunan ferik (FRAP). Aktiviti ketoksikan sel mendedahkan ekstrak metanol berpotensi untuk menindas garisan sel Caco-2 dengan peratusan daya maju sel pada 70% berbanding garisan sel MCF-7. Aktiviti antioksidan daripada ujian kuasa antioksidan penurunan ferik mencatatkan nilainya sebanyak 47.8080 µg TE/gDW manakala 13.8481 µg/mL untuk IC50 daripada aktiviti penghapusan radikal. Walau bagaimanapun, jumlah kandungan flavonoid (TFC) tidak dikesan dalam ekstrak metanol C. fenestratum. Ujian antibakteria mendedahkan bahawa ekstrak telah menghalang pertumbuhan E. chrysanthemi dan E. carotovora secara sederhana pada 400 mg/mL dengan zon perencatan masing-masing 11.50 mm dan 11.83 mm. Oleh itu, ekstrak metanol daripada C. fenestratum berpotensi bertindak sebagai agen antioksida dan anti reput lembut tetapi kurang berpotensi sebagai agen anti kanser dan anti reput buah.
format	Article
author	Harun, Aiza Abdul Aziz, Noorshilawati Daud, Shaari Salim, Khairil Syazwan Abu Bakar, Norlaili Mat So'Ad, Siti Zaiton Ahmat, Norizan
author_facet	Harun, Aiza Abdul Aziz, Noorshilawati Daud, Shaari Salim, Khairil Syazwan Abu Bakar, Norlaili Mat So'Ad, Siti Zaiton Ahmat, Norizan
author_sort	Harun, Aiza
title	Cytotoxicity study, antioxidant and antibacterial activity against plant pathogenic bacteria of methanol extract from Coscinium fenestratum (akar sekunyit) = Kajian ketoksikan sel, aktiviti antioksida dan sifat antibakteria ekstrak metanol dari Coscinium fenestratum (akar sekunyit) terhadap bakteria penyebab penyakit pokok
title_short	Cytotoxicity study, antioxidant and antibacterial activity against plant pathogenic bacteria of methanol extract from Coscinium fenestratum (akar sekunyit) = Kajian ketoksikan sel, aktiviti antioksida dan sifat antibakteria ekstrak metanol dari Coscinium fenestratum (akar sekunyit) terhadap bakteria penyebab penyakit pokok
title_full	Cytotoxicity study, antioxidant and antibacterial activity against plant pathogenic bacteria of methanol extract from Coscinium fenestratum (akar sekunyit) = Kajian ketoksikan sel, aktiviti antioksida dan sifat antibakteria ekstrak metanol dari Coscinium fenestratum (akar sekunyit) terhadap bakteria penyebab penyakit pokok
title_fullStr	Cytotoxicity study, antioxidant and antibacterial activity against plant pathogenic bacteria of methanol extract from Coscinium fenestratum (akar sekunyit) = Kajian ketoksikan sel, aktiviti antioksida dan sifat antibakteria ekstrak metanol dari Coscinium fenestratum (akar sekunyit) terhadap bakteria penyebab penyakit pokok
title_full_unstemmed	Cytotoxicity study, antioxidant and antibacterial activity against plant pathogenic bacteria of methanol extract from Coscinium fenestratum (akar sekunyit) = Kajian ketoksikan sel, aktiviti antioksida dan sifat antibakteria ekstrak metanol dari Coscinium fenestratum (akar sekunyit) terhadap bakteria penyebab penyakit pokok
title_sort	cytotoxicity study, antioxidant and antibacterial activity against plant pathogenic bacteria of methanol extract from coscinium fenestratum (akar sekunyit) = kajian ketoksikan sel, aktiviti antioksida dan sifat antibakteria ekstrak metanol dari coscinium fenestratum (akar sekunyit) terhadap bakteria penyebab penyakit pokok
publisher	Malaysian Analytical Sciences Society (ANALIS)
publishDate	2023
url	http://irep.iium.edu.my/111484/1/111484_Cytotoxicity%20study%2C%20antioxidant%20and%20antibacterial%20activity.pdf http://irep.iium.edu.my/111484/2/111484_Cytotoxicity%20study%2C%20antioxidant%20and%20antibacterial%20activity_SCOPUS.pdf http://irep.iium.edu.my/111484/ https://mjas.analis.com.my/mjas/v27_n6/v27_n6.html
_version_	1794545520588357632
spelling	my.iium.irep.1114842024-03-22T07:58:12Z http://irep.iium.edu.my/111484/ Cytotoxicity study, antioxidant and antibacterial activity against plant pathogenic bacteria of methanol extract from Coscinium fenestratum (akar sekunyit) = Kajian ketoksikan sel, aktiviti antioksida dan sifat antibakteria ekstrak metanol dari Coscinium fenestratum (akar sekunyit) terhadap bakteria penyebab penyakit pokok Harun, Aiza Abdul Aziz, Noorshilawati Daud, Shaari Salim, Khairil Syazwan Abu Bakar, Norlaili Mat So'Ad, Siti Zaiton Ahmat, Norizan QD Chemistry Coscinium fenestratum (Gaertn.) Colebr. (Menispermaceae) is a large woody liana found in moist deciduous to the evergreen forest at an altitude of 350–1200m. The stem and root of this species are highly medicinal valuable, and it is believed to be an endangered species in Malaysia. This study was conducted to investigate the reaction of methanol extract from Coscinium fenestratum with cancer cell lines, plant pathogenic bacteria, as well as DPPH scavenging and ferric reduction antioxidant power. The antibacterial activity was evaluated from disc diffusion method and microdilution method against soft rot bacteria Erwinia chrysanthemi and Erwinia carotovora. The cytotoxicity assay was performed using MCF-7 and Caco-2 cancer cell lines. The antioxidant properties of methanol extract were assessed from DPPH radical scavenging activity and ferric reducing antioxidant power (FRAP). The cytotoxicity activity revealed the methanol extract has potential to suppress the Caco-2 cell line with the percentage cell viability at 70% compared to MCF-7 cell line. The antioxidant activity from FRAP assay recorded its value of 47.8080 µg for TE/gDW and 13.8481 µg/mL for the IC50 from radical scavenging activity. However, the total flavonoid content (TFC) was not detected in methanol extract of C. fenestratum. The antibacterial assay revealed that the extract had moderately inhibited the growth of E. chrysanthemi and E. carotovora at 400 mg/mL, with inhibition zones of 11.50 mm and 11.83 mm, respectively. Therefore, the methanol extract from C. fenestratum has potential to act as antioxidant and anti-soft rot agent but less potential as anti-cancer agent and anti-soft rot agent. ***** Coscinium fenestratum (Gaertn.) Colebr. (Menispermaceae), pokok liana berkayu besar yang ditemui dalam hutan daun luruh lembap hingga ke hutan malar hijau pada ketinggian 350–1200 m. Batang dan akar spesis ini sangat bernilai dalam bidang perubatan, dan ia dipercayai spesis terancam di Malaysia. Kajian ini bertujuan untuk mengkaji tindakan ekstrak metanol daripada Coscinium fenestratum bertindak balas dengan garisan sel kanser, bakteria patogen tumbuhan serta penghapusan DPPH dan kuasa antioksidan penurunan ferik (FRAP). Aktiviti antibakteria dinilai daripada kaedah resapan cakera dan kaedah pencairan mikro terhadap bakteria reput lembut Erwinia chrysanthemi dan Erwinia carotovora. Ujian ketoksikan sel telah diadakan menggunakan garisan sel kanser MCF-7 dan Caco-2. Sifat antioksidan ekstrak metanol dinilai daripada aktiviti penghapusan radikal DPPH dan kuasa antioksidan penurunan ferik (FRAP). Aktiviti ketoksikan sel mendedahkan ekstrak metanol berpotensi untuk menindas garisan sel Caco-2 dengan peratusan daya maju sel pada 70% berbanding garisan sel MCF-7. Aktiviti antioksidan daripada ujian kuasa antioksidan penurunan ferik mencatatkan nilainya sebanyak 47.8080 µg TE/gDW manakala 13.8481 µg/mL untuk IC50 daripada aktiviti penghapusan radikal. Walau bagaimanapun, jumlah kandungan flavonoid (TFC) tidak dikesan dalam ekstrak metanol C. fenestratum. Ujian antibakteria mendedahkan bahawa ekstrak telah menghalang pertumbuhan E. chrysanthemi dan E. carotovora secara sederhana pada 400 mg/mL dengan zon perencatan masing-masing 11.50 mm dan 11.83 mm. Oleh itu, ekstrak metanol daripada C. fenestratum berpotensi bertindak sebagai agen antioksida dan anti reput lembut tetapi kurang berpotensi sebagai agen anti kanser dan anti reput buah. Malaysian Analytical Sciences Society (ANALIS) 2023-12-29 Article PeerReviewed application/pdf en http://irep.iium.edu.my/111484/1/111484_Cytotoxicity%20study%2C%20antioxidant%20and%20antibacterial%20activity.pdf application/pdf en http://irep.iium.edu.my/111484/2/111484_Cytotoxicity%20study%2C%20antioxidant%20and%20antibacterial%20activity_SCOPUS.pdf Harun, Aiza and Abdul Aziz, Noorshilawati and Daud, Shaari and Salim, Khairil Syazwan and Abu Bakar, Norlaili and Mat So'Ad, Siti Zaiton and Ahmat, Norizan (2023) Cytotoxicity study, antioxidant and antibacterial activity against plant pathogenic bacteria of methanol extract from Coscinium fenestratum (akar sekunyit) = Kajian ketoksikan sel, aktiviti antioksida dan sifat antibakteria ekstrak metanol dari Coscinium fenestratum (akar sekunyit) terhadap bakteria penyebab penyakit pokok. Malaysian Journal of Analytical Sciences, 27 (6). pp. 1249-1256. ISSN 1394-2506 https://mjas.analis.com.my/mjas/v27_n6/v27_n6.html
score	13.211869

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