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Enzymatic hydrolysis for the removal of 3- monochloropropanediol esters in edible oils using Candida rugosa lipase in the presence of deep eutectic solvents and nanocellulose

3-monochloropropanediol ester (3-MCPDE) is identified as a food-borne contaminant in edible oils and are classified as a possible carcinogen. This study reports an efficient enzymatic technique for the removal of 3-MCPDE from extra virgin olive oil (EVO) using Candida rugosa lipase (CRL) as the bioc...

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Main Authors: Syed Putra, Sharifah Shahira, Basirun, Wan Jefrey, Hayyan, Adeeb, Elgharbawy, Amal A.M.
Format: Article
Language:English
English
Published: Elsevier B.V. 2023
Subjects:
QD Chemistry
Online Access:http://irep.iium.edu.my/104641/4/104641_Enzymatic%20hydrolysis.pdf
http://irep.iium.edu.my/104641/20/104641_Enzymatic%20hydrolysis_SCOPUS.pdf
http://irep.iium.edu.my/104641/
https://www.sciencedirect.com/science/article/abs/pii/S1369703X23001535
https://doi.org/10.1016/j.bej.2023.108958
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spelling my.iium.irep.1046412024-05-17T03:04:38Z http://irep.iium.edu.my/104641/ Enzymatic hydrolysis for the removal of 3- monochloropropanediol esters in edible oils using Candida rugosa lipase in the presence of deep eutectic solvents and nanocellulose Syed Putra, Sharifah Shahira Basirun, Wan Jefrey Hayyan, Adeeb Elgharbawy, Amal A.M. QD Chemistry 3-monochloropropanediol ester (3-MCPDE) is identified as a food-borne contaminant in edible oils and are classified as a possible carcinogen. This study reports an efficient enzymatic technique for the removal of 3-MCPDE from extra virgin olive oil (EVO) using Candida rugosa lipase (CRL) as the biocatalyst in the presence of choline chloride and fructose-based natural deep eutectic solvent (NADES) and nanocellulose (NC) extracted from almond shells. The validity of the method was confirmed by gas chromatography mass spectrometry (GC-MS) showing adequate precision with relative standard deviation values ≤ 2.37%. The quantification and detection limit are within the permissible levels of 3-MCPD in edible oils. Under optimized conditions of 30 min at 90 °C with 60 µL of phenylboronic acid (PBA), the enzymatic hydrolysis resulted in the removal of 79.8% of spiked 3-MCPD in EVO. No adverse effects of the EVO were detected from this technique with respect to the oil quality testing. The application of DESs and NCs as the support material for the CRL biocatalyst for the removal of 3-MCPDE has yet to be explored. This could have a significant impact on the edible oil industry for producing oils of higher quality free from 3-MCPDE. Elsevier B.V. 2023-04-27 Article PeerReviewed application/pdf en http://irep.iium.edu.my/104641/4/104641_Enzymatic%20hydrolysis.pdf application/pdf en http://irep.iium.edu.my/104641/20/104641_Enzymatic%20hydrolysis_SCOPUS.pdf Syed Putra, Sharifah Shahira and Basirun, Wan Jefrey and Hayyan, Adeeb and Elgharbawy, Amal A.M. (2023) Enzymatic hydrolysis for the removal of 3- monochloropropanediol esters in edible oils using Candida rugosa lipase in the presence of deep eutectic solvents and nanocellulose. Biochemical Engineering Journal, 196. pp. 1-11. ISSN 1369-703X E-ISSN 1873-295X https://www.sciencedirect.com/science/article/abs/pii/S1369703X23001535 https://doi.org/10.1016/j.bej.2023.108958
institution Universiti Islam Antarabangsa Malaysia
building IIUM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider International Islamic University Malaysia
content_source IIUM Repository (IREP)
url_provider http://irep.iium.edu.my/
language English
English
topic QD Chemistry
spellingShingle QD Chemistry
Syed Putra, Sharifah Shahira
Basirun, Wan Jefrey
Hayyan, Adeeb
Elgharbawy, Amal A.M.
Enzymatic hydrolysis for the removal of 3- monochloropropanediol esters in edible oils using Candida rugosa lipase in the presence of deep eutectic solvents and nanocellulose
description 3-monochloropropanediol ester (3-MCPDE) is identified as a food-borne contaminant in edible oils and are classified as a possible carcinogen. This study reports an efficient enzymatic technique for the removal of 3-MCPDE from extra virgin olive oil (EVO) using Candida rugosa lipase (CRL) as the biocatalyst in the presence of choline chloride and fructose-based natural deep eutectic solvent (NADES) and nanocellulose (NC) extracted from almond shells. The validity of the method was confirmed by gas chromatography mass spectrometry (GC-MS) showing adequate precision with relative standard deviation values ≤ 2.37%. The quantification and detection limit are within the permissible levels of 3-MCPD in edible oils. Under optimized conditions of 30 min at 90 °C with 60 µL of phenylboronic acid (PBA), the enzymatic hydrolysis resulted in the removal of 79.8% of spiked 3-MCPD in EVO. No adverse effects of the EVO were detected from this technique with respect to the oil quality testing. The application of DESs and NCs as the support material for the CRL biocatalyst for the removal of 3-MCPDE has yet to be explored. This could have a significant impact on the edible oil industry for producing oils of higher quality free from 3-MCPDE.
format Article
author Syed Putra, Sharifah Shahira
Basirun, Wan Jefrey
Hayyan, Adeeb
Elgharbawy, Amal A.M.
author_facet Syed Putra, Sharifah Shahira
Basirun, Wan Jefrey
Hayyan, Adeeb
Elgharbawy, Amal A.M.
author_sort Syed Putra, Sharifah Shahira
title Enzymatic hydrolysis for the removal of 3- monochloropropanediol esters in edible oils using Candida rugosa lipase in the presence of deep eutectic solvents and nanocellulose
title_short Enzymatic hydrolysis for the removal of 3- monochloropropanediol esters in edible oils using Candida rugosa lipase in the presence of deep eutectic solvents and nanocellulose
title_full Enzymatic hydrolysis for the removal of 3- monochloropropanediol esters in edible oils using Candida rugosa lipase in the presence of deep eutectic solvents and nanocellulose
title_fullStr Enzymatic hydrolysis for the removal of 3- monochloropropanediol esters in edible oils using Candida rugosa lipase in the presence of deep eutectic solvents and nanocellulose
title_full_unstemmed Enzymatic hydrolysis for the removal of 3- monochloropropanediol esters in edible oils using Candida rugosa lipase in the presence of deep eutectic solvents and nanocellulose
title_sort enzymatic hydrolysis for the removal of 3- monochloropropanediol esters in edible oils using candida rugosa lipase in the presence of deep eutectic solvents and nanocellulose
publisher Elsevier B.V.
publishDate 2023
url http://irep.iium.edu.my/104641/4/104641_Enzymatic%20hydrolysis.pdf
http://irep.iium.edu.my/104641/20/104641_Enzymatic%20hydrolysis_SCOPUS.pdf
http://irep.iium.edu.my/104641/
https://www.sciencedirect.com/science/article/abs/pii/S1369703X23001535
https://doi.org/10.1016/j.bej.2023.108958
_version_ 1800081756233138176
score 13.244745

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