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Screening of catechol dioxygenase gene among bacterial communities isolated from anthropogenic contaminated area in Pahang, Malaysia

The development of industrial, recreational, and urban areas in Pahang has introduced hydrocarbon pollution into the environment. The hydrocarbons impart a significant effect on the environment and human health, in both short- and long-term effects. Longterm exposure to these environmental pollutan...

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Main Authors: Azizan, Nur Hafizah, Sharif, Mohd. Faez, Abdul Aziz, Asmadamia, Zulbahri, Azuan Syafiq
Format: Article
Language:English
English
Published: Malaysian Society for Biochemistry and Molecular Biology 2022
Subjects:
Q Science (General)
QR Microbiology
Online Access:http://irep.iium.edu.my/103187/1/103187_Screening%20of%20catechol%20dioxygenase%20gene.pdf
http://irep.iium.edu.my/103187/7/103187_Screening%20of%20catechol%20dioxygenase%20gene_SCOPUS.pdf
http://irep.iium.edu.my/103187/
https://msbmb2010.wixsite.com/mjbmb/iciabc-2021
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spelling my.iium.irep.1031872023-11-23T04:08:53Z http://irep.iium.edu.my/103187/ Screening of catechol dioxygenase gene among bacterial communities isolated from anthropogenic contaminated area in Pahang, Malaysia Azizan, Nur Hafizah Sharif, Mohd. Faez Abdul Aziz, Asmadamia Zulbahri, Azuan Syafiq Q Science (General) QR Microbiology The development of industrial, recreational, and urban areas in Pahang has introduced hydrocarbon pollution into the environment. The hydrocarbons impart a significant effect on the environment and human health, in both short- and long-term effects. Longterm exposure to these environmental pollutants may enhance the degradative abilities of the indigenous bacterial communities through the exhibition of catechol dioxygenase; an enzyme that can degrade hydrocarbons. In this study, bacteria communities that are tolerant towards hydrocarbons were successfully isolated and identified from two contaminated rivers and one pristine area. The presence of the catechol dioxygenase gene from the isolated bacterial samples was then screened using Polymerase Chain Reaction (PCR) amplification. A total of 33 hydrocarbon-tolerant bacteria were isolated from both contaminated and pristine areas. Five genera isolated from the contaminated areas; the Bacillus, Lysinibacillus, Aeromonas, Shewanella, and Pseudomonas strains were detected to harbour the catechol dioxygenase gene. Meanwhile, no catechol dioxygenase gene was detected in bacterial samples isolated from pristine area. Results obtained from the screening of the catechol dioxygenase gene can provide preliminary insight regarding the presence of catabolic enzymes particularly in different anthropogenic areas, which could provide a better understanding regarding the potential of catechol dioxygenase in eliminating toxic pollutants from the environment. Malaysian Society for Biochemistry and Molecular Biology 2022-12 Article PeerReviewed application/pdf en http://irep.iium.edu.my/103187/1/103187_Screening%20of%20catechol%20dioxygenase%20gene.pdf application/pdf en http://irep.iium.edu.my/103187/7/103187_Screening%20of%20catechol%20dioxygenase%20gene_SCOPUS.pdf Azizan, Nur Hafizah and Sharif, Mohd. Faez and Abdul Aziz, Asmadamia and Zulbahri, Azuan Syafiq (2022) Screening of catechol dioxygenase gene among bacterial communities isolated from anthropogenic contaminated area in Pahang, Malaysia. Malaysian Journal of Biochemistry & Molecular Biology, Special Issue (2022) (1). pp. 78-86. ISSN 1511-2616 https://msbmb2010.wixsite.com/mjbmb/iciabc-2021
institution Universiti Islam Antarabangsa Malaysia
building IIUM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider International Islamic University Malaysia
content_source IIUM Repository (IREP)
url_provider http://irep.iium.edu.my/
language English
English
topic Q Science (General)
QR Microbiology
spellingShingle Q Science (General)
QR Microbiology
Azizan, Nur Hafizah
Sharif, Mohd. Faez
Abdul Aziz, Asmadamia
Zulbahri, Azuan Syafiq
Screening of catechol dioxygenase gene among bacterial communities isolated from anthropogenic contaminated area in Pahang, Malaysia
description The development of industrial, recreational, and urban areas in Pahang has introduced hydrocarbon pollution into the environment. The hydrocarbons impart a significant effect on the environment and human health, in both short- and long-term effects. Longterm exposure to these environmental pollutants may enhance the degradative abilities of the indigenous bacterial communities through the exhibition of catechol dioxygenase; an enzyme that can degrade hydrocarbons. In this study, bacteria communities that are tolerant towards hydrocarbons were successfully isolated and identified from two contaminated rivers and one pristine area. The presence of the catechol dioxygenase gene from the isolated bacterial samples was then screened using Polymerase Chain Reaction (PCR) amplification. A total of 33 hydrocarbon-tolerant bacteria were isolated from both contaminated and pristine areas. Five genera isolated from the contaminated areas; the Bacillus, Lysinibacillus, Aeromonas, Shewanella, and Pseudomonas strains were detected to harbour the catechol dioxygenase gene. Meanwhile, no catechol dioxygenase gene was detected in bacterial samples isolated from pristine area. Results obtained from the screening of the catechol dioxygenase gene can provide preliminary insight regarding the presence of catabolic enzymes particularly in different anthropogenic areas, which could provide a better understanding regarding the potential of catechol dioxygenase in eliminating toxic pollutants from the environment.
format Article
author Azizan, Nur Hafizah
Sharif, Mohd. Faez
Abdul Aziz, Asmadamia
Zulbahri, Azuan Syafiq
author_facet Azizan, Nur Hafizah
Sharif, Mohd. Faez
Abdul Aziz, Asmadamia
Zulbahri, Azuan Syafiq
author_sort Azizan, Nur Hafizah
title Screening of catechol dioxygenase gene among bacterial communities isolated from anthropogenic contaminated area in Pahang, Malaysia
title_short Screening of catechol dioxygenase gene among bacterial communities isolated from anthropogenic contaminated area in Pahang, Malaysia
title_full Screening of catechol dioxygenase gene among bacterial communities isolated from anthropogenic contaminated area in Pahang, Malaysia
title_fullStr Screening of catechol dioxygenase gene among bacterial communities isolated from anthropogenic contaminated area in Pahang, Malaysia
title_full_unstemmed Screening of catechol dioxygenase gene among bacterial communities isolated from anthropogenic contaminated area in Pahang, Malaysia
title_sort screening of catechol dioxygenase gene among bacterial communities isolated from anthropogenic contaminated area in pahang, malaysia
publisher Malaysian Society for Biochemistry and Molecular Biology
publishDate 2022
url http://irep.iium.edu.my/103187/1/103187_Screening%20of%20catechol%20dioxygenase%20gene.pdf
http://irep.iium.edu.my/103187/7/103187_Screening%20of%20catechol%20dioxygenase%20gene_SCOPUS.pdf
http://irep.iium.edu.my/103187/
https://msbmb2010.wixsite.com/mjbmb/iciabc-2021
_version_ 1783876079981690880
score 13.211869

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