Screening of catechol dioxygenase gene among bacterial communities isolated from anthropogenic contaminated area in Pahang, Malaysia
The development of industrial, recreational, and urban areas in Pahang has introduced hydrocarbon pollution into the environment. The hydrocarbons impart a significant effect on the environment and human health, in both short- and long-term effects. Longterm exposure to these environmental pollutan...
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| Main Authors: | , , , |
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| Format: | Article |
| Language: | English English |
| Published: |
Malaysian Society for Biochemistry and Molecular Biology
2022
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| Subjects: | |
| Online Access: | http://irep.iium.edu.my/103187/1/103187_Screening%20of%20catechol%20dioxygenase%20gene.pdf http://irep.iium.edu.my/103187/7/103187_Screening%20of%20catechol%20dioxygenase%20gene_SCOPUS.pdf http://irep.iium.edu.my/103187/ https://msbmb2010.wixsite.com/mjbmb/iciabc-2021 |
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| Summary: | The development of industrial, recreational, and urban areas in Pahang has introduced hydrocarbon pollution into the environment. The hydrocarbons impart a significant
effect on the environment and human health, in both short- and long-term effects. Longterm exposure to these environmental pollutants may enhance the degradative abilities of the indigenous bacterial communities through the exhibition of catechol dioxygenase; an enzyme that can degrade hydrocarbons. In this study, bacteria communities that are tolerant towards hydrocarbons were successfully isolated and identified from two contaminated rivers and one pristine area. The presence of the catechol dioxygenase gene from the isolated bacterial samples was then screened using
Polymerase Chain Reaction (PCR) amplification. A total of 33 hydrocarbon-tolerant bacteria were isolated from both contaminated and pristine areas. Five genera isolated
from the contaminated areas; the Bacillus, Lysinibacillus, Aeromonas, Shewanella, and Pseudomonas strains were detected to harbour the catechol dioxygenase gene.
Meanwhile, no catechol dioxygenase gene was detected in bacterial samples isolated from pristine area. Results obtained from the screening of the catechol dioxygenase
gene can provide preliminary insight regarding the presence of catabolic enzymes particularly in different anthropogenic areas, which could provide a better understanding regarding the potential of catechol dioxygenase in eliminating toxic
pollutants from the environment. |
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