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Identification and Characterization of The Causal Agent of Infected Iceberg Lettuce (Lactuca Sativa L.) in Perak, Malaysia.

Lack of knowledge about the causal agent of infected crops lead farmers to use wrong treatment on their crops, which is causing negative effects on environment and human’s health. This study was conducted to identify and characterize the causal agent from the infected iceberg lettuce (Lactuca sati...

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Main Authors: Noor Afiza, Badaluddin, Saiful Iskandar, Khalit, Mohammad Hailmi, Sajili
Format: Article
Language:English
Published: 2019
Subjects:
QH301 Biology
Online Access:http://eprints.unisza.edu.my/6537/1/FH02-FBIM-19-31679.pdf
http://eprints.unisza.edu.my/6537/
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spelling my-unisza-ir.65372022-05-22T02:19:48Z http://eprints.unisza.edu.my/6537/ Identification and Characterization of The Causal Agent of Infected Iceberg Lettuce (Lactuca Sativa L.) in Perak, Malaysia. Noor Afiza, Badaluddin Saiful Iskandar, Khalit Mohammad Hailmi, Sajili QH301 Biology Lack of knowledge about the causal agent of infected crops lead farmers to use wrong treatment on their crops, which is causing negative effects on environment and human’s health. This study was conducted to identify and characterize the causal agent from the infected iceberg lettuce (Lactuca sativa L.) in Perak, Malaysia. The infected iceberg lettuces were sampling from Perak area and the causal agent was isolated by direct plating technique on a nutrient medium. The isolated bacteria were identified by molecular method and biochemical test. Molecular identification was carried out using 16S rRNA sequence and then, several tests such as amylase, catalase, oxidase and others were done for identification of bacterial species based on their biochemical activities. The obtained results showed that Serratia marcescenes and Stenotrophomonas sp. were identified as the causal agents. The pathogenicity has been confirmed by the establishment of Koch’s Postulate, which showed 100% disease infection on them. The environmental effects also have been tested on the bacteria to study the ability of bacteria to grow in different environmental conditions based on different in pH, salinity, and temperature. All the bacterial sample showed that they able to growth on the pH between 4.0 to 9.0, at the temperature 10 °C to 45 °C and at the addition salinity to the medium between 2.5 %, 5.0% and 7.5 %. 2019-10 Article PeerReviewed text en http://eprints.unisza.edu.my/6537/1/FH02-FBIM-19-31679.pdf Noor Afiza, Badaluddin and Saiful Iskandar, Khalit and Mohammad Hailmi, Sajili (2019) Identification and Characterization of The Causal Agent of Infected Iceberg Lettuce (Lactuca Sativa L.) in Perak, Malaysia. Journal of Agrobiotechnology, 10 (1S). pp. 59-67. ISSN 2180-1983
institution Universiti Sultan Zainal Abidin
building UNISZA Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Sultan Zainal Abidin
content_source UNISZA Institutional Repository
url_provider https://eprints.unisza.edu.my/
language English
topic QH301 Biology
spellingShingle QH301 Biology
Noor Afiza, Badaluddin
Saiful Iskandar, Khalit
Mohammad Hailmi, Sajili
Identification and Characterization of The Causal Agent of Infected Iceberg Lettuce (Lactuca Sativa L.) in Perak, Malaysia.
description Lack of knowledge about the causal agent of infected crops lead farmers to use wrong treatment on their crops, which is causing negative effects on environment and human’s health. This study was conducted to identify and characterize the causal agent from the infected iceberg lettuce (Lactuca sativa L.) in Perak, Malaysia. The infected iceberg lettuces were sampling from Perak area and the causal agent was isolated by direct plating technique on a nutrient medium. The isolated bacteria were identified by molecular method and biochemical test. Molecular identification was carried out using 16S rRNA sequence and then, several tests such as amylase, catalase, oxidase and others were done for identification of bacterial species based on their biochemical activities. The obtained results showed that Serratia marcescenes and Stenotrophomonas sp. were identified as the causal agents. The pathogenicity has been confirmed by the establishment of Koch’s Postulate, which showed 100% disease infection on them. The environmental effects also have been tested on the bacteria to study the ability of bacteria to grow in different environmental conditions based on different in pH, salinity, and temperature. All the bacterial sample showed that they able to growth on the pH between 4.0 to 9.0, at the temperature 10 °C to 45 °C and at the addition salinity to the medium between 2.5 %, 5.0% and 7.5 %.
format Article
author Noor Afiza, Badaluddin
Saiful Iskandar, Khalit
Mohammad Hailmi, Sajili
author_facet Noor Afiza, Badaluddin
Saiful Iskandar, Khalit
Mohammad Hailmi, Sajili
author_sort Noor Afiza, Badaluddin
title Identification and Characterization of The Causal Agent of Infected Iceberg Lettuce (Lactuca Sativa L.) in Perak, Malaysia.
title_short Identification and Characterization of The Causal Agent of Infected Iceberg Lettuce (Lactuca Sativa L.) in Perak, Malaysia.
title_full Identification and Characterization of The Causal Agent of Infected Iceberg Lettuce (Lactuca Sativa L.) in Perak, Malaysia.
title_fullStr Identification and Characterization of The Causal Agent of Infected Iceberg Lettuce (Lactuca Sativa L.) in Perak, Malaysia.
title_full_unstemmed Identification and Characterization of The Causal Agent of Infected Iceberg Lettuce (Lactuca Sativa L.) in Perak, Malaysia.
title_sort identification and characterization of the causal agent of infected iceberg lettuce (lactuca sativa l.) in perak, malaysia.
publishDate 2019
url http://eprints.unisza.edu.my/6537/1/FH02-FBIM-19-31679.pdf
http://eprints.unisza.edu.my/6537/
_version_ 1734304565583413248
score 13.211869

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