Identification and Characterization of The Causal Agent of Infected Iceberg Lettuce (Lactuca Sativa L.) in Perak, Malaysia.
Lack of knowledge about the causal agent of infected crops lead farmers to use wrong treatment on their crops, which is causing negative effects on environment and human’s health. This study was conducted to identify and characterize the causal agent from the infected iceberg lettuce (Lactuca sati...
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| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
| Published: |
2019
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| Subjects: | |
| Online Access: | http://eprints.unisza.edu.my/6537/1/FH02-FBIM-19-31679.pdf http://eprints.unisza.edu.my/6537/ |
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| Summary: | Lack of knowledge about the causal agent of infected crops lead farmers to use wrong treatment on their
crops, which is causing negative effects on environment and human’s health. This study was conducted to
identify and characterize the causal agent from the infected iceberg lettuce (Lactuca sativa L.) in Perak,
Malaysia. The infected iceberg lettuces were sampling from Perak area and the causal agent was isolated by
direct plating technique on a nutrient medium. The isolated bacteria were identified by molecular method
and biochemical test. Molecular identification was carried out using 16S rRNA sequence and then, several
tests such as amylase, catalase, oxidase and others were done for identification of bacterial species based on
their biochemical activities. The obtained results showed that Serratia marcescenes and Stenotrophomonas sp. were
identified as the causal agents. The pathogenicity has been confirmed by the establishment of Koch’s
Postulate, which showed 100% disease infection on them. The environmental effects also have been tested
on the bacteria to study the ability of bacteria to grow in different environmental conditions based on
different in pH, salinity, and temperature. All the bacterial sample showed that they able to growth on the
pH between 4.0 to 9.0, at the temperature 10 °C to 45 °C and at the addition salinity to the medium between
2.5 %, 5.0% and 7.5 %. |
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