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Detection, Isolation and Antimicrobial Testing of Listeria monocytogenes in Chicken from Supermarket

Listeria monocytogenes play a vital role in causing an infrequent and sometimes deadly foodborne disease known as Listeriosis. In this study, the presence of L. monocytogenes in chicken at supermarket and the antimicrobial susceptibility of the pathogen were determined. Detection of L. monocytogenes...

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Main Authors: John Tang, Yew Huat, Noor Afiza, Badaluddin, Aisyah Amirah, Ismail
Format: Article
Language:English
Published: 2018
Subjects:
Q Science (General)
Online Access:http://eprints.unisza.edu.my/5807/1/FH02-FBIM-19-24108.pdf
http://eprints.unisza.edu.my/5807/
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spelling my-unisza-ir.58072022-02-27T02:28:24Z http://eprints.unisza.edu.my/5807/ Detection, Isolation and Antimicrobial Testing of Listeria monocytogenes in Chicken from Supermarket John Tang, Yew Huat Noor Afiza, Badaluddin Aisyah Amirah, Ismail Q Science (General) Listeria monocytogenes play a vital role in causing an infrequent and sometimes deadly foodborne disease known as Listeriosis. In this study, the presence of L. monocytogenes in chicken at supermarket and the antimicrobial susceptibility of the pathogen were determined. Detection of L. monocytogenes in chickens from supermarket was carried out using real time PCR and isolation of L. monocytogenes by plating onto PALCAM agar. The L. monocytogenes isolates were tested against 14 antibiotics for resistance determination using disc diffusion method. From 56 chicken samples tested, 35.7 % of the samples were detected contained the pathogen using real time PCR. Only 15 samples (26.8%) were positive for L. monocytogenes isolates. The isolates showed high resistance towards Penicillin G (100%), Ampicillin (93%), Rifampicin (93%), Ceftazidime (87%), Erythromycin (87%), and Tetracycline (80%). All the isolates were found to have the Multiple Antibiotic Resistance (MAR) index more than 0.2 which indicates multiple resistance towards antibiotics. In conclu-sion, L. monocytogenes was found in the chicken at supermarket and the isolates were found to be highly resistance towards multiple antibiotics. Thus, chicken meat must be properly cooked before consumption to prevent foodborne infection. 2018-12 Article PeerReviewed text en http://eprints.unisza.edu.my/5807/1/FH02-FBIM-19-24108.pdf John Tang, Yew Huat and Noor Afiza, Badaluddin and Aisyah Amirah, Ismail (2018) Detection, Isolation and Antimicrobial Testing of Listeria monocytogenes in Chicken from Supermarket. International Journal of Engineering & Technology, 7 (4). pp. 113-116. ISSN 2227-524X
institution Universiti Sultan Zainal Abidin
building UNISZA Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Sultan Zainal Abidin
content_source UNISZA Institutional Repository
url_provider https://eprints.unisza.edu.my/
language English
topic Q Science (General)
spellingShingle Q Science (General)
John Tang, Yew Huat
Noor Afiza, Badaluddin
Aisyah Amirah, Ismail
Detection, Isolation and Antimicrobial Testing of Listeria monocytogenes in Chicken from Supermarket
description Listeria monocytogenes play a vital role in causing an infrequent and sometimes deadly foodborne disease known as Listeriosis. In this study, the presence of L. monocytogenes in chicken at supermarket and the antimicrobial susceptibility of the pathogen were determined. Detection of L. monocytogenes in chickens from supermarket was carried out using real time PCR and isolation of L. monocytogenes by plating onto PALCAM agar. The L. monocytogenes isolates were tested against 14 antibiotics for resistance determination using disc diffusion method. From 56 chicken samples tested, 35.7 % of the samples were detected contained the pathogen using real time PCR. Only 15 samples (26.8%) were positive for L. monocytogenes isolates. The isolates showed high resistance towards Penicillin G (100%), Ampicillin (93%), Rifampicin (93%), Ceftazidime (87%), Erythromycin (87%), and Tetracycline (80%). All the isolates were found to have the Multiple Antibiotic Resistance (MAR) index more than 0.2 which indicates multiple resistance towards antibiotics. In conclu-sion, L. monocytogenes was found in the chicken at supermarket and the isolates were found to be highly resistance towards multiple antibiotics. Thus, chicken meat must be properly cooked before consumption to prevent foodborne infection.
format Article
author John Tang, Yew Huat
Noor Afiza, Badaluddin
Aisyah Amirah, Ismail
author_facet John Tang, Yew Huat
Noor Afiza, Badaluddin
Aisyah Amirah, Ismail
author_sort John Tang, Yew Huat
title Detection, Isolation and Antimicrobial Testing of Listeria monocytogenes in Chicken from Supermarket
title_short Detection, Isolation and Antimicrobial Testing of Listeria monocytogenes in Chicken from Supermarket
title_full Detection, Isolation and Antimicrobial Testing of Listeria monocytogenes in Chicken from Supermarket
title_fullStr Detection, Isolation and Antimicrobial Testing of Listeria monocytogenes in Chicken from Supermarket
title_full_unstemmed Detection, Isolation and Antimicrobial Testing of Listeria monocytogenes in Chicken from Supermarket
title_sort detection, isolation and antimicrobial testing of listeria monocytogenes in chicken from supermarket
publishDate 2018
url http://eprints.unisza.edu.my/5807/1/FH02-FBIM-19-24108.pdf
http://eprints.unisza.edu.my/5807/
_version_ 1725976996394041344
score 13.211869

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