Staff View: The effects of individual and combination of asiatic acid and madecassoside derived from Centella asiatica (Linn.) on the viability percentage and morphological changes of mouse macrophage cell lines (J774A.1)

The effects of individual and combination of asiatic acid and madecassoside derived from Centella asiatica (Linn.) on the viability percentage and morphological changes of mouse macrophage cell lines (J774A.1)

Asiatic acid and madecassoside are the two pentacyclic triterpenoid compounds derived from Centella asiatica (Linn.), which believed to possess major contribution in many related pharmacological activities. This research is conducted to determine the viability and the morphological changes of macr...

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Main Authors:	Nurul Hikmah, Harun, Wan Amir Nizam, Wan Ahmad, Rapeah, Suppıan
Format:	Article
Language:	English
Published:	2018
Subjects:	R Medicine (General) RM Therapeutics. Pharmacology
Online Access:	http://eprints.unisza.edu.my/5520/1/FH02-FSK-20-40891.pdf http://eprints.unisza.edu.my/5520/
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id	my-unisza-ir.5520
record_format	eprints
spelling	my-unisza-ir.55202022-02-17T01:08:38Z http://eprints.unisza.edu.my/5520/ The effects of individual and combination of asiatic acid and madecassoside derived from Centella asiatica (Linn.) on the viability percentage and morphological changes of mouse macrophage cell lines (J774A.1) Nurul Hikmah, Harun Wan Amir Nizam, Wan Ahmad Rapeah, Suppıan R Medicine (General) RM Therapeutics. Pharmacology Asiatic acid and madecassoside are the two pentacyclic triterpenoid compounds derived from Centella asiatica (Linn.), which believed to possess major contribution in many related pharmacological activities. This research is conducted to determine the viability and the morphological changes of macrophage cells after treating with individual and combination of the mentioned compounds by using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay and observing through image analyzer system, respectively. Results displayed that only AA at high doses 25 and 50 μg/ml, exhibited mild inhibition of mouse macrophage cells J774A.1 viability with only the latter dose able to alter the morphology of the cells after 24 hours of treatment. Therefore, both compounds either in sole or mix form were harmless to the cells (IC50 > 50 μg/ml). Interestingly, the combination treatment also enhanced the macrophage cell viability higher than their sole treatment in every concentration. As a consequence, the bioactive compounds at none-cytotoxic level can be applied in any in-vitro further studies such as immunomodulatory and antiinflammatory in order to prove the local traditional claim on the herb and for future benefit in a new prospective of natural product-based drugs development. 2018-09 Article PeerReviewed text en http://eprints.unisza.edu.my/5520/1/FH02-FSK-20-40891.pdf Nurul Hikmah, Harun and Wan Amir Nizam, Wan Ahmad and Rapeah, Suppıan (2018) The effects of individual and combination of asiatic acid and madecassoside derived from Centella asiatica (Linn.) on the viability percentage and morphological changes of mouse macrophage cell lines (J774A.1). Journal of applied pharmaceutical science, 8 (11). pp. 109-115. ISSN 2231-3354
institution	Universiti Sultan Zainal Abidin
building	UNISZA Library
collection	Institutional Repository
continent	Asia
country	Malaysia
content_provider	Universiti Sultan Zainal Abidin
content_source	UNISZA Institutional Repository
url_provider	https://eprints.unisza.edu.my/
language	English
topic	R Medicine (General) RM Therapeutics. Pharmacology
spellingShingle	R Medicine (General) RM Therapeutics. Pharmacology Nurul Hikmah, Harun Wan Amir Nizam, Wan Ahmad Rapeah, Suppıan The effects of individual and combination of asiatic acid and madecassoside derived from Centella asiatica (Linn.) on the viability percentage and morphological changes of mouse macrophage cell lines (J774A.1)
description	Asiatic acid and madecassoside are the two pentacyclic triterpenoid compounds derived from Centella asiatica (Linn.), which believed to possess major contribution in many related pharmacological activities. This research is conducted to determine the viability and the morphological changes of macrophage cells after treating with individual and combination of the mentioned compounds by using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay and observing through image analyzer system, respectively. Results displayed that only AA at high doses 25 and 50 μg/ml, exhibited mild inhibition of mouse macrophage cells J774A.1 viability with only the latter dose able to alter the morphology of the cells after 24 hours of treatment. Therefore, both compounds either in sole or mix form were harmless to the cells (IC50 > 50 μg/ml). Interestingly, the combination treatment also enhanced the macrophage cell viability higher than their sole treatment in every concentration. As a consequence, the bioactive compounds at none-cytotoxic level can be applied in any in-vitro further studies such as immunomodulatory and antiinflammatory in order to prove the local traditional claim on the herb and for future benefit in a new prospective of natural product-based drugs development.
format	Article
author	Nurul Hikmah, Harun Wan Amir Nizam, Wan Ahmad Rapeah, Suppıan
author_facet	Nurul Hikmah, Harun Wan Amir Nizam, Wan Ahmad Rapeah, Suppıan
author_sort	Nurul Hikmah, Harun
title	The effects of individual and combination of asiatic acid and madecassoside derived from Centella asiatica (Linn.) on the viability percentage and morphological changes of mouse macrophage cell lines (J774A.1)
title_short	The effects of individual and combination of asiatic acid and madecassoside derived from Centella asiatica (Linn.) on the viability percentage and morphological changes of mouse macrophage cell lines (J774A.1)
title_full	The effects of individual and combination of asiatic acid and madecassoside derived from Centella asiatica (Linn.) on the viability percentage and morphological changes of mouse macrophage cell lines (J774A.1)
title_fullStr	The effects of individual and combination of asiatic acid and madecassoside derived from Centella asiatica (Linn.) on the viability percentage and morphological changes of mouse macrophage cell lines (J774A.1)
title_full_unstemmed	The effects of individual and combination of asiatic acid and madecassoside derived from Centella asiatica (Linn.) on the viability percentage and morphological changes of mouse macrophage cell lines (J774A.1)
title_sort	effects of individual and combination of asiatic acid and madecassoside derived from centella asiatica (linn.) on the viability percentage and morphological changes of mouse macrophage cell lines (j774a.1)
publishDate	2018
url	http://eprints.unisza.edu.my/5520/1/FH02-FSK-20-40891.pdf http://eprints.unisza.edu.my/5520/
_version_	1725976965806030848
score	13.211869

The effects of individual and combination of asiatic acid and madecassoside derived from Centella asiatica (Linn.) on the viability percentage and morphological changes of mouse macrophage cell lines (J774A.1)

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