The effects of individual and combination of asiatic acid and madecassoside derived from Centella asiatica (Linn.) on the viability percentage and morphological changes of mouse macrophage cell lines (J774A.1)
Asiatic acid and madecassoside are the two pentacyclic triterpenoid compounds derived from Centella asiatica (Linn.), which believed to possess major contribution in many related pharmacological activities. This research is conducted to determine the viability and the morphological changes of macr...
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| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
| Published: |
2018
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| Subjects: | |
| Online Access: | http://eprints.unisza.edu.my/5520/1/FH02-FSK-20-40891.pdf http://eprints.unisza.edu.my/5520/ |
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| Summary: | Asiatic acid and madecassoside are the two pentacyclic triterpenoid compounds derived from Centella asiatica
(Linn.), which believed to possess major contribution in many related pharmacological activities. This research is
conducted to determine the viability and the morphological changes of macrophage cells after treating with individual
and combination of the mentioned compounds by using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium
bromide assay and observing through image analyzer system, respectively. Results displayed that only AA at high
doses 25 and 50 μg/ml, exhibited mild inhibition of mouse macrophage cells J774A.1 viability with only the latter
dose able to alter the morphology of the cells after 24 hours of treatment. Therefore, both compounds either in sole
or mix form were harmless to the cells (IC50 > 50 μg/ml). Interestingly, the combination treatment also enhanced the
macrophage cell viability higher than their sole treatment in every concentration. As a consequence, the bioactive
compounds at none-cytotoxic level can be applied in any in-vitro further studies such as immunomodulatory and antiinflammatory
in order to prove the local traditional claim on the herb and for future benefit in a new prospective of
natural product-based drugs development. |
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