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Standardization of Orthoshiphon stamineus raw material and extracts for anti-uterine fibroid

This study aim to evaluate gravimetric parameters and physicochemical of raw materials of Orthosiphon stamineus in order to ascertain the quality of plant materials and achieved standardized material. Anti-uterine fibroid potential was investigated by cytotoxicity test on uterine leiomyosarcoma ce...

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Main Authors: Mohd, K.S., Halim, N.H., Pauzi, N., Hamil, S.H.R., Shafaei, A., Ismail, Z.
Format: Article
Language:English
Published: 2017
Subjects:
QH301 Biology
Online Access:http://eprints.unisza.edu.my/5292/1/FH02-FBIM-17-08890.pdf
http://eprints.unisza.edu.my/5292/
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spelling my-unisza-ir.52922022-02-10T01:54:45Z http://eprints.unisza.edu.my/5292/ Standardization of Orthoshiphon stamineus raw material and extracts for anti-uterine fibroid Mohd, K.S. Halim, N.H. Pauzi, N. Hamil, S.H.R. Shafaei, A. Ismail, Z. QH301 Biology This study aim to evaluate gravimetric parameters and physicochemical of raw materials of Orthosiphon stamineus in order to ascertain the quality of plant materials and achieved standardized material. Anti-uterine fibroid potential was investigated by cytotoxicity test on uterine leiomyosarcoma cell SK-UT-1. Gravimetric analysis showed that all parameters adhered to the standard according to Malaysian Herbal Monograph. Primary and secondary metabolites screening revealed that aqueous extract have highest total glycosaponins, total phenolics and total phospholipids with the amounts of 27.38%, 18.75% and 3.17%, respectively. Meanwhile, 50% ethanolic extract have highest total proteins, total polysaccharides and total flavonoid with amounts of 9.2%, 19.79%, 8.13%, respectively. Evaluation of cytotoxic potency showed that aqueous extract was more cytotoxic compared to 50% ethanolic extract with the IC50 18µg/mL and 34µg/mL, respectively. Mode of cell death analysis by AO/PI double staining substantiated apoptosis induction by water extract. The results of this study can serve as reference data for standardization of O. stamineus extracts for development as anti-uterine fibroid. 2017-01 Article PeerReviewed text en http://eprints.unisza.edu.my/5292/1/FH02-FBIM-17-08890.pdf Mohd, K.S. and Halim, N.H. and Pauzi, N. and Hamil, S.H.R. and Shafaei, A. and Ismail, Z. (2017) Standardization of Orthoshiphon stamineus raw material and extracts for anti-uterine fibroid. International Journal of Pharmacognosy and Phytochemical Research, 9 (4). pp. 512-515. ISSN 0975-4873
institution Universiti Sultan Zainal Abidin
building UNISZA Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Sultan Zainal Abidin
content_source UNISZA Institutional Repository
url_provider https://eprints.unisza.edu.my/
language English
topic QH301 Biology
spellingShingle QH301 Biology
Mohd, K.S.
Halim, N.H.
Pauzi, N.
Hamil, S.H.R.
Shafaei, A.
Ismail, Z.
Standardization of Orthoshiphon stamineus raw material and extracts for anti-uterine fibroid
description This study aim to evaluate gravimetric parameters and physicochemical of raw materials of Orthosiphon stamineus in order to ascertain the quality of plant materials and achieved standardized material. Anti-uterine fibroid potential was investigated by cytotoxicity test on uterine leiomyosarcoma cell SK-UT-1. Gravimetric analysis showed that all parameters adhered to the standard according to Malaysian Herbal Monograph. Primary and secondary metabolites screening revealed that aqueous extract have highest total glycosaponins, total phenolics and total phospholipids with the amounts of 27.38%, 18.75% and 3.17%, respectively. Meanwhile, 50% ethanolic extract have highest total proteins, total polysaccharides and total flavonoid with amounts of 9.2%, 19.79%, 8.13%, respectively. Evaluation of cytotoxic potency showed that aqueous extract was more cytotoxic compared to 50% ethanolic extract with the IC50 18µg/mL and 34µg/mL, respectively. Mode of cell death analysis by AO/PI double staining substantiated apoptosis induction by water extract. The results of this study can serve as reference data for standardization of O. stamineus extracts for development as anti-uterine fibroid.
format Article
author Mohd, K.S.
Halim, N.H.
Pauzi, N.
Hamil, S.H.R.
Shafaei, A.
Ismail, Z.
author_facet Mohd, K.S.
Halim, N.H.
Pauzi, N.
Hamil, S.H.R.
Shafaei, A.
Ismail, Z.
author_sort Mohd, K.S.
title Standardization of Orthoshiphon stamineus raw material and extracts for anti-uterine fibroid
title_short Standardization of Orthoshiphon stamineus raw material and extracts for anti-uterine fibroid
title_full Standardization of Orthoshiphon stamineus raw material and extracts for anti-uterine fibroid
title_fullStr Standardization of Orthoshiphon stamineus raw material and extracts for anti-uterine fibroid
title_full_unstemmed Standardization of Orthoshiphon stamineus raw material and extracts for anti-uterine fibroid
title_sort standardization of orthoshiphon stamineus raw material and extracts for anti-uterine fibroid
publishDate 2017
url http://eprints.unisza.edu.my/5292/1/FH02-FBIM-17-08890.pdf
http://eprints.unisza.edu.my/5292/
_version_ 1724613038635483136
score 13.211869

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