Standardization of Orthoshiphon stamineus raw material and extracts for anti-uterine fibroid
This study aim to evaluate gravimetric parameters and physicochemical of raw materials of Orthosiphon stamineus in order to ascertain the quality of plant materials and achieved standardized material. Anti-uterine fibroid potential was investigated by cytotoxicity test on uterine leiomyosarcoma ce...
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| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
2017
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| Subjects: | |
| Online Access: | http://eprints.unisza.edu.my/5292/1/FH02-FBIM-17-08890.pdf http://eprints.unisza.edu.my/5292/ |
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| Summary: | This study aim to evaluate gravimetric parameters and physicochemical of raw materials of Orthosiphon stamineus in order
to ascertain the quality of plant materials and achieved standardized material. Anti-uterine fibroid potential was investigated
by cytotoxicity test on uterine leiomyosarcoma cell SK-UT-1. Gravimetric analysis showed that all parameters adhered to
the standard according to Malaysian Herbal Monograph. Primary and secondary metabolites screening revealed that
aqueous extract have highest total glycosaponins, total phenolics and total phospholipids with the amounts of 27.38%,
18.75% and 3.17%, respectively. Meanwhile, 50% ethanolic extract have highest total proteins, total polysaccharides and
total flavonoid with amounts of 9.2%, 19.79%, 8.13%, respectively. Evaluation of cytotoxic potency showed that aqueous
extract was more cytotoxic compared to 50% ethanolic extract with the IC50 18µg/mL and 34µg/mL, respectively. Mode
of cell death analysis by AO/PI double staining substantiated apoptosis induction by water extract. The results of this study
can serve as reference data for standardization of O. stamineus extracts for development as anti-uterine fibroid. |
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