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Antibiotic resistance profile and RAPD analysis of Campylobacter jejuni isolated from vegetables farms and retail markets

Objective: To investigate antibiotic resistance profile and characterize Campylobacter jejuni (C. jejuni) isolates using random amplified polymorphic DNA (RAPD) analysis. Methods: Ninety eight C. jejuni isolates from farms and retail outlets were screened against 10 antibiotics commonly used clin...

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Main Authors: Huat, J.T.Y., Che Abdullah, Abu Bakar, Mohd Ikhsan, Khalid, Syazana Aimi, ., Son, Radu
Format: Article
Language:English
Published: 2015
Subjects:
Q Science (General)
QH301 Biology
Online Access:http://eprints.unisza.edu.my/5071/1/FH02-FBIM-16-05225.pdf
http://eprints.unisza.edu.my/5071/
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spelling my-unisza-ir.50712022-05-22T07:46:28Z http://eprints.unisza.edu.my/5071/ Antibiotic resistance profile and RAPD analysis of Campylobacter jejuni isolated from vegetables farms and retail markets Huat, J.T.Y. Che Abdullah, Abu Bakar Mohd Ikhsan, Khalid Syazana Aimi, . Son, Radu Q Science (General) QH301 Biology Objective: To investigate antibiotic resistance profile and characterize Campylobacter jejuni (C. jejuni) isolates using random amplified polymorphic DNA (RAPD) analysis. Methods: Ninety eight C. jejuni isolates from farms and retail outlets were screened against 10 antibiotics commonly used clinically and agriculturally by using disk diffusion method. RAPD analysis was done to characterize 98 C. jejuni isolates. Results: Fifty-one percent of the isolates had multiple antibiotic resistance index 0.2 and below. This indicated that the isolates in the vegetables were not from the high risk environment or extensive farming practices. C. jejuni isolates found resistant towards penicillin G (93%), vancomycin (86%), ampicillin (35%), erythromycin (28%), gentamycin (4%), amikacin (3%), enrofloxacin (1%), norfloxacin (1%) and no resistance towards ciprofloxacin. RAPD clustering analysis showed that the contamination of C. jejuni in vegetables was likely due to cross contamination at retail markets. Conclusions: C. jejuni contamination in vegetables at retail markets was due to cross contamination. Current finding proved that C. jejuni in small scale vegetables production was less expose towards antibiotic abuse. 2015-12 Article PeerReviewed text en http://eprints.unisza.edu.my/5071/1/FH02-FBIM-16-05225.pdf Huat, J.T.Y. and Che Abdullah, Abu Bakar and Mohd Ikhsan, Khalid and Syazana Aimi, . and Son, Radu (2015) Antibiotic resistance profile and RAPD analysis of Campylobacter jejuni isolated from vegetables farms and retail markets. Asian Pacific Journal of Tropical Biomedicine, 6 (1). pp. 71-75. ISSN 2221-1691
institution Universiti Sultan Zainal Abidin
building UNISZA Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Sultan Zainal Abidin
content_source UNISZA Institutional Repository
url_provider https://eprints.unisza.edu.my/
language English
topic Q Science (General)
QH301 Biology
spellingShingle Q Science (General)
QH301 Biology
Huat, J.T.Y.
Che Abdullah, Abu Bakar
Mohd Ikhsan, Khalid
Syazana Aimi, .
Son, Radu
Antibiotic resistance profile and RAPD analysis of Campylobacter jejuni isolated from vegetables farms and retail markets
description Objective: To investigate antibiotic resistance profile and characterize Campylobacter jejuni (C. jejuni) isolates using random amplified polymorphic DNA (RAPD) analysis. Methods: Ninety eight C. jejuni isolates from farms and retail outlets were screened against 10 antibiotics commonly used clinically and agriculturally by using disk diffusion method. RAPD analysis was done to characterize 98 C. jejuni isolates. Results: Fifty-one percent of the isolates had multiple antibiotic resistance index 0.2 and below. This indicated that the isolates in the vegetables were not from the high risk environment or extensive farming practices. C. jejuni isolates found resistant towards penicillin G (93%), vancomycin (86%), ampicillin (35%), erythromycin (28%), gentamycin (4%), amikacin (3%), enrofloxacin (1%), norfloxacin (1%) and no resistance towards ciprofloxacin. RAPD clustering analysis showed that the contamination of C. jejuni in vegetables was likely due to cross contamination at retail markets. Conclusions: C. jejuni contamination in vegetables at retail markets was due to cross contamination. Current finding proved that C. jejuni in small scale vegetables production was less expose towards antibiotic abuse.
format Article
author Huat, J.T.Y.
Che Abdullah, Abu Bakar
Mohd Ikhsan, Khalid
Syazana Aimi, .
Son, Radu
author_facet Huat, J.T.Y.
Che Abdullah, Abu Bakar
Mohd Ikhsan, Khalid
Syazana Aimi, .
Son, Radu
author_sort Huat, J.T.Y.
title Antibiotic resistance profile and RAPD analysis of Campylobacter jejuni isolated from vegetables farms and retail markets
title_short Antibiotic resistance profile and RAPD analysis of Campylobacter jejuni isolated from vegetables farms and retail markets
title_full Antibiotic resistance profile and RAPD analysis of Campylobacter jejuni isolated from vegetables farms and retail markets
title_fullStr Antibiotic resistance profile and RAPD analysis of Campylobacter jejuni isolated from vegetables farms and retail markets
title_full_unstemmed Antibiotic resistance profile and RAPD analysis of Campylobacter jejuni isolated from vegetables farms and retail markets
title_sort antibiotic resistance profile and rapd analysis of campylobacter jejuni isolated from vegetables farms and retail markets
publishDate 2015
url http://eprints.unisza.edu.my/5071/1/FH02-FBIM-16-05225.pdf
http://eprints.unisza.edu.my/5071/
_version_ 1734304554784129024
score 13.211869

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