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Antihyperlipidemic and hepatoprotective effect of zingiber cassumunar rhizome extract in high-fat diet-induced hyperlipidemic rats: the role of antioxidant activity

Hyperlipidemia and oxidative stress are major risk factors for the onset of cardiovascular diseases, and the oxidative stress caused by high level of lipids can cause liver damage. Zingiber cassumunar has been reported to contain a high antioxidant content that may provide therapeutic advantages. Th...

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Main Authors: Mahfudh, Nurkhasanah, Sulistyani, Nanik, Sari, Novita, Sari, Indah Puspati, Adrian, Dedi, Fezah Othman,, Zainul Amiruddin Zakaria,
Format: Article
Language:English
Published: Penerbit Universiti Kebangsaan Malaysia 2023
Online Access:http://journalarticle.ukm.my/23084/1/SMS%207.pdf
http://journalarticle.ukm.my/23084/
https://www.ukm.my/jsm/english_journals/vol52num9_2023/contentsVol52num9_2023.html
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spelling my-ukm.journal.230842024-02-16T04:08:18Z http://journalarticle.ukm.my/23084/ Antihyperlipidemic and hepatoprotective effect of zingiber cassumunar rhizome extract in high-fat diet-induced hyperlipidemic rats: the role of antioxidant activity Mahfudh, Nurkhasanah Sulistyani, Nanik Sari, Novita Sari, Indah Puspati Adrian, Dedi Fezah Othman, Zainul Amiruddin Zakaria, Hyperlipidemia and oxidative stress are major risk factors for the onset of cardiovascular diseases, and the oxidative stress caused by high level of lipids can cause liver damage. Zingiber cassumunar has been reported to contain a high antioxidant content that may provide therapeutic advantages. The present study was to evaluate the antihyperlipidemic and hepatoprotective effects of Z. cassumunar rhizome extract (ZCRE) in high-fat diet (HFD)-induced hyperlipidemic rats model and investigate the mechanism through its effect on the endogenous antioxidant enzymes. In this study, the rhizomes of Z. cassumunar was extracted using ethanol 96% (v/v) and evaporated to get the concentrated Z. cassumunar rhizome extract (ZCRE). Thin layer chromatography (TLC)-densitometry was performed to determine the curcumin content in the extract. High fat diet-induced hyperlipidemia model was used to evaluate the anti-hyperlipidemic and hepatoprotective activities of ZCRE in rats. Male Wistar rats were randomly divided into five groups: normal control; High fat diet-induced hyperlipidemic rats (HFD); High Fat Diet and 100 mg/kgBW of ZCRE (HFD + 100 mg/ kgBW); High Fat Diet and 200 mg/kgBW of ZCRE (HFD + 200 mg/kgBW); and High Fat Diet and 400 mg/kgBW of ZCRE (HFD + 400 mg/kgBW). The antihyperlipidemic and hepatoprotective potential of ZCRE were assessed through a series of analyses of body weight, blood biochemical parameters, which include total cholesterol (TC), triglycerides (TG), the serum glutamic-oxaloacetic transaminase (SGOT) and serum glutamic-pyruvic transaminase (SGPT). The antioxidant activity of catalase (CAT) and glutation peroxidase (GSH-Px) were assessed on the liver homogenate. Data of the study were presented as mean ± SD and analyzed by using one way analysis of variance (ANOVA) followed by Least Significant Difference (LSD) test for multiple comparisons. The TLC analysis showed that ZCRE contained a significant amount of Curcumin. In addition, the study has also shown that ZRCE was able to significantly lower the levels of total cholesterol, triglyceride, SGPT, and SGOT as compared to hyperlipidemic rats (p<0.05) Concomitantly, the activity of CAT and GSH-Px was found significantly increased (p<0.05) when compared to hyperlipidemic control, with the dose of 400 mg/kg BW being the most effective. This study showed the significant antihyperlipidemic and hepatoprotective effects of ZCRE in HFD-induced hyperlipidemic rats, which mechanism might possibly connect to the increased antioxidant enzyme activities. Penerbit Universiti Kebangsaan Malaysia 2023 Article PeerReviewed application/pdf en http://journalarticle.ukm.my/23084/1/SMS%207.pdf Mahfudh, Nurkhasanah and Sulistyani, Nanik and Sari, Novita and Sari, Indah Puspati and Adrian, Dedi and Fezah Othman, and Zainul Amiruddin Zakaria, (2023) Antihyperlipidemic and hepatoprotective effect of zingiber cassumunar rhizome extract in high-fat diet-induced hyperlipidemic rats: the role of antioxidant activity. Sains Malaysiana, 52 (9). pp. 2559-2569. ISSN 0126-6039 https://www.ukm.my/jsm/english_journals/vol52num9_2023/contentsVol52num9_2023.html
institution Universiti Kebangsaan Malaysia
building Tun Sri Lanang Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Kebangsaan Malaysia
content_source UKM Journal Article Repository
url_provider http://journalarticle.ukm.my/
language English
description Hyperlipidemia and oxidative stress are major risk factors for the onset of cardiovascular diseases, and the oxidative stress caused by high level of lipids can cause liver damage. Zingiber cassumunar has been reported to contain a high antioxidant content that may provide therapeutic advantages. The present study was to evaluate the antihyperlipidemic and hepatoprotective effects of Z. cassumunar rhizome extract (ZCRE) in high-fat diet (HFD)-induced hyperlipidemic rats model and investigate the mechanism through its effect on the endogenous antioxidant enzymes. In this study, the rhizomes of Z. cassumunar was extracted using ethanol 96% (v/v) and evaporated to get the concentrated Z. cassumunar rhizome extract (ZCRE). Thin layer chromatography (TLC)-densitometry was performed to determine the curcumin content in the extract. High fat diet-induced hyperlipidemia model was used to evaluate the anti-hyperlipidemic and hepatoprotective activities of ZCRE in rats. Male Wistar rats were randomly divided into five groups: normal control; High fat diet-induced hyperlipidemic rats (HFD); High Fat Diet and 100 mg/kgBW of ZCRE (HFD + 100 mg/ kgBW); High Fat Diet and 200 mg/kgBW of ZCRE (HFD + 200 mg/kgBW); and High Fat Diet and 400 mg/kgBW of ZCRE (HFD + 400 mg/kgBW). The antihyperlipidemic and hepatoprotective potential of ZCRE were assessed through a series of analyses of body weight, blood biochemical parameters, which include total cholesterol (TC), triglycerides (TG), the serum glutamic-oxaloacetic transaminase (SGOT) and serum glutamic-pyruvic transaminase (SGPT). The antioxidant activity of catalase (CAT) and glutation peroxidase (GSH-Px) were assessed on the liver homogenate. Data of the study were presented as mean ± SD and analyzed by using one way analysis of variance (ANOVA) followed by Least Significant Difference (LSD) test for multiple comparisons. The TLC analysis showed that ZCRE contained a significant amount of Curcumin. In addition, the study has also shown that ZRCE was able to significantly lower the levels of total cholesterol, triglyceride, SGPT, and SGOT as compared to hyperlipidemic rats (p<0.05) Concomitantly, the activity of CAT and GSH-Px was found significantly increased (p<0.05) when compared to hyperlipidemic control, with the dose of 400 mg/kg BW being the most effective. This study showed the significant antihyperlipidemic and hepatoprotective effects of ZCRE in HFD-induced hyperlipidemic rats, which mechanism might possibly connect to the increased antioxidant enzyme activities.
format Article
author Mahfudh, Nurkhasanah
Sulistyani, Nanik
Sari, Novita
Sari, Indah Puspati
Adrian, Dedi
Fezah Othman,
Zainul Amiruddin Zakaria,
spellingShingle Mahfudh, Nurkhasanah
Sulistyani, Nanik
Sari, Novita
Sari, Indah Puspati
Adrian, Dedi
Fezah Othman,
Zainul Amiruddin Zakaria,
Antihyperlipidemic and hepatoprotective effect of zingiber cassumunar rhizome extract in high-fat diet-induced hyperlipidemic rats: the role of antioxidant activity
author_facet Mahfudh, Nurkhasanah
Sulistyani, Nanik
Sari, Novita
Sari, Indah Puspati
Adrian, Dedi
Fezah Othman,
Zainul Amiruddin Zakaria,
author_sort Mahfudh, Nurkhasanah
title Antihyperlipidemic and hepatoprotective effect of zingiber cassumunar rhizome extract in high-fat diet-induced hyperlipidemic rats: the role of antioxidant activity
title_short Antihyperlipidemic and hepatoprotective effect of zingiber cassumunar rhizome extract in high-fat diet-induced hyperlipidemic rats: the role of antioxidant activity
title_full Antihyperlipidemic and hepatoprotective effect of zingiber cassumunar rhizome extract in high-fat diet-induced hyperlipidemic rats: the role of antioxidant activity
title_fullStr Antihyperlipidemic and hepatoprotective effect of zingiber cassumunar rhizome extract in high-fat diet-induced hyperlipidemic rats: the role of antioxidant activity
title_full_unstemmed Antihyperlipidemic and hepatoprotective effect of zingiber cassumunar rhizome extract in high-fat diet-induced hyperlipidemic rats: the role of antioxidant activity
title_sort antihyperlipidemic and hepatoprotective effect of zingiber cassumunar rhizome extract in high-fat diet-induced hyperlipidemic rats: the role of antioxidant activity
publisher Penerbit Universiti Kebangsaan Malaysia
publishDate 2023
url http://journalarticle.ukm.my/23084/1/SMS%207.pdf
http://journalarticle.ukm.my/23084/
https://www.ukm.my/jsm/english_journals/vol52num9_2023/contentsVol52num9_2023.html
_version_ 1792152151282155520
score 13.211869

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