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Molecular characterization and pathogenicity of stagonosporopsis cucurbitacearum causing gummy stem blight disease of watermelon (Citrullus lanatus L.) in Malaysia

Watermelon (Citrullus lanatus L.) is a popular fruit crop with high economic value and widely grown in Malaysia. In January 2020, gummy stem blight (GSB) has become a threat for production of watermelon in Malaysia particularly in warm and humid climates, but the causative agent of GSB infecting wat...

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Main Authors: Abdul Aziz Waziri,, Dzarifah Zulperi,, Siti Izera Ismail,
Format: Article
Language:English
Published: Universiti Kebangsaan Malaysia 2023
Online Access:http://journalarticle.ukm.my/21907/1/ST%201.pdf
http://journalarticle.ukm.my/21907/
http://www.ukm.my/jsm/index.html
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spelling my-ukm.journal.219072023-07-21T01:15:16Z http://journalarticle.ukm.my/21907/ Molecular characterization and pathogenicity of stagonosporopsis cucurbitacearum causing gummy stem blight disease of watermelon (Citrullus lanatus L.) in Malaysia Abdul Aziz Waziri, Dzarifah Zulperi, Siti Izera Ismail, Watermelon (Citrullus lanatus L.) is a popular fruit crop with high economic value and widely grown in Malaysia. In January 2020, gummy stem blight (GSB) has become a threat for production of watermelon in Malaysia particularly in warm and humid climates, but the causative agent of GSB infecting watermelon is unknown. This disease decreases the fresh fruit marketability. Watermelon plants cultivar Red Rocky showing varied degree (40-90%) of suspected GSB, were collected from two main watermelon growing areas in Malaysia. Initial symptoms appeared as marginal brown necrotic lesions on leaves, while on the stems showed water-soaked, necrotic lesions and exuded reddish-brown gummy exudate on the stems. A total of ten isolates were isolated from lesions on leaves and stems of watermelon plants affected by GSB. All ten fungal isolates were identified as Stagonosporopsis cucurbitacearum on the basis of morphological characteristics and phylogenetic analysis of combined sequences of the internal transcribed spacer (ITS) and β-tubulin regions. All isolates were proven to be pathogenic when inoculated on the leaves and stems of the watermelon plant and the the fungal isolates were consistently reisolated from the diseased watermelon plants confirming Koch’s postulates. Pathogenicity tests indicated that there were significant differences in virulence among the S. cucurbitacearum when inoculated on the leaves and stems of the watermelon plant. Understanding the etiology of the pathogen will help in disease management of gummy stem blight disease in Malaysia. Universiti Kebangsaan Malaysia 2023 Article PeerReviewed application/pdf en http://journalarticle.ukm.my/21907/1/ST%201.pdf Abdul Aziz Waziri, and Dzarifah Zulperi, and Siti Izera Ismail, (2023) Molecular characterization and pathogenicity of stagonosporopsis cucurbitacearum causing gummy stem blight disease of watermelon (Citrullus lanatus L.) in Malaysia. Sains Malaysiana, 52 (3). pp. 683-691. ISSN 0126-6039 http://www.ukm.my/jsm/index.html
institution Universiti Kebangsaan Malaysia
building Tun Sri Lanang Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Kebangsaan Malaysia
content_source UKM Journal Article Repository
url_provider http://journalarticle.ukm.my/
language English
description Watermelon (Citrullus lanatus L.) is a popular fruit crop with high economic value and widely grown in Malaysia. In January 2020, gummy stem blight (GSB) has become a threat for production of watermelon in Malaysia particularly in warm and humid climates, but the causative agent of GSB infecting watermelon is unknown. This disease decreases the fresh fruit marketability. Watermelon plants cultivar Red Rocky showing varied degree (40-90%) of suspected GSB, were collected from two main watermelon growing areas in Malaysia. Initial symptoms appeared as marginal brown necrotic lesions on leaves, while on the stems showed water-soaked, necrotic lesions and exuded reddish-brown gummy exudate on the stems. A total of ten isolates were isolated from lesions on leaves and stems of watermelon plants affected by GSB. All ten fungal isolates were identified as Stagonosporopsis cucurbitacearum on the basis of morphological characteristics and phylogenetic analysis of combined sequences of the internal transcribed spacer (ITS) and β-tubulin regions. All isolates were proven to be pathogenic when inoculated on the leaves and stems of the watermelon plant and the the fungal isolates were consistently reisolated from the diseased watermelon plants confirming Koch’s postulates. Pathogenicity tests indicated that there were significant differences in virulence among the S. cucurbitacearum when inoculated on the leaves and stems of the watermelon plant. Understanding the etiology of the pathogen will help in disease management of gummy stem blight disease in Malaysia.
format Article
author Abdul Aziz Waziri,
Dzarifah Zulperi,
Siti Izera Ismail,
spellingShingle Abdul Aziz Waziri,
Dzarifah Zulperi,
Siti Izera Ismail,
Molecular characterization and pathogenicity of stagonosporopsis cucurbitacearum causing gummy stem blight disease of watermelon (Citrullus lanatus L.) in Malaysia
author_facet Abdul Aziz Waziri,
Dzarifah Zulperi,
Siti Izera Ismail,
author_sort Abdul Aziz Waziri,
title Molecular characterization and pathogenicity of stagonosporopsis cucurbitacearum causing gummy stem blight disease of watermelon (Citrullus lanatus L.) in Malaysia
title_short Molecular characterization and pathogenicity of stagonosporopsis cucurbitacearum causing gummy stem blight disease of watermelon (Citrullus lanatus L.) in Malaysia
title_full Molecular characterization and pathogenicity of stagonosporopsis cucurbitacearum causing gummy stem blight disease of watermelon (Citrullus lanatus L.) in Malaysia
title_fullStr Molecular characterization and pathogenicity of stagonosporopsis cucurbitacearum causing gummy stem blight disease of watermelon (Citrullus lanatus L.) in Malaysia
title_full_unstemmed Molecular characterization and pathogenicity of stagonosporopsis cucurbitacearum causing gummy stem blight disease of watermelon (Citrullus lanatus L.) in Malaysia
title_sort molecular characterization and pathogenicity of stagonosporopsis cucurbitacearum causing gummy stem blight disease of watermelon (citrullus lanatus l.) in malaysia
publisher Universiti Kebangsaan Malaysia
publishDate 2023
url http://journalarticle.ukm.my/21907/1/ST%201.pdf
http://journalarticle.ukm.my/21907/
http://www.ukm.my/jsm/index.html
_version_ 1772812461766868992
score 13.211869

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