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Streptococcus pneumoniae infection increases expression of von Willebrand factor (vWF) in a mouse brain endothelium model

von Willebrand factor (vWF) is a large multimeric glycoprotein which is normally released by the brain endothelial cells and is therefore present as a circulating protein in the blood. The initial and mature structure of vWF is uncleaved; however, pneumococcal infection can cause the cleavage of...

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Main Authors: Nurul Adhwa Rahman,, SuwarnI D,, Siti Hanna M,
Format: Article
Language:English
Published: Pusat Perubatan Universiti Kebangsaan Malaysia 2018
Online Access:http://journalarticle.ukm.my/20206/1/13_ms0211_pdf_56811.pdf
http://journalarticle.ukm.my/20206/
https://www.medicineandhealthukm.com/toc/13/1
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spelling my-ukm.journal.202062022-10-21T08:23:36Z http://journalarticle.ukm.my/20206/ Streptococcus pneumoniae infection increases expression of von Willebrand factor (vWF) in a mouse brain endothelium model Nurul Adhwa Rahman, SuwarnI D, Siti Hanna M, von Willebrand factor (vWF) is a large multimeric glycoprotein which is normally released by the brain endothelial cells and is therefore present as a circulating protein in the blood. The initial and mature structure of vWF is uncleaved; however, pneumococcal infection can cause the cleavage of the protein, thus formingseveral multimers at different sizes. The present study tested the hypothesis that Streptococcus pneumoniae infection of brain endothelial cells in culture induces the release of vWF at certain sizes time-dependently. Immortalized mouse brain endothelial cells (bEnd.3) were infected at specific hours with Streptococcus pneumoniae strains (serotype 19F). Immunofluorescence microscopy was performed and quantified accordingly. Cells were also extracted, and the proteins were separated into different sizes by western blot. The expression of vWF was then analysed and compared for a potential relationship between the strains and between the specific time of infection. The expression of vWF was elevated during infection. Even though multiple and non-specific events can also cause the cleavage of vWF, this study showed that the cleavage pattern of vWF is potentially dependent on the incubation time of infection. Pusat Perubatan Universiti Kebangsaan Malaysia 2018 Article PeerReviewed application/pdf en http://journalarticle.ukm.my/20206/1/13_ms0211_pdf_56811.pdf Nurul Adhwa Rahman, and SuwarnI D, and Siti Hanna M, (2018) Streptococcus pneumoniae infection increases expression of von Willebrand factor (vWF) in a mouse brain endothelium model. Medicine & Health, 13 (1). pp. 130-142. ISSN 2289-5728 https://www.medicineandhealthukm.com/toc/13/1
institution Universiti Kebangsaan Malaysia
building Tun Sri Lanang Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Kebangsaan Malaysia
content_source UKM Journal Article Repository
url_provider http://journalarticle.ukm.my/
language English
description von Willebrand factor (vWF) is a large multimeric glycoprotein which is normally released by the brain endothelial cells and is therefore present as a circulating protein in the blood. The initial and mature structure of vWF is uncleaved; however, pneumococcal infection can cause the cleavage of the protein, thus formingseveral multimers at different sizes. The present study tested the hypothesis that Streptococcus pneumoniae infection of brain endothelial cells in culture induces the release of vWF at certain sizes time-dependently. Immortalized mouse brain endothelial cells (bEnd.3) were infected at specific hours with Streptococcus pneumoniae strains (serotype 19F). Immunofluorescence microscopy was performed and quantified accordingly. Cells were also extracted, and the proteins were separated into different sizes by western blot. The expression of vWF was then analysed and compared for a potential relationship between the strains and between the specific time of infection. The expression of vWF was elevated during infection. Even though multiple and non-specific events can also cause the cleavage of vWF, this study showed that the cleavage pattern of vWF is potentially dependent on the incubation time of infection.
format Article
author Nurul Adhwa Rahman,
SuwarnI D,
Siti Hanna M,
spellingShingle Nurul Adhwa Rahman,
SuwarnI D,
Siti Hanna M,
Streptococcus pneumoniae infection increases expression of von Willebrand factor (vWF) in a mouse brain endothelium model
author_facet Nurul Adhwa Rahman,
SuwarnI D,
Siti Hanna M,
author_sort Nurul Adhwa Rahman,
title Streptococcus pneumoniae infection increases expression of von Willebrand factor (vWF) in a mouse brain endothelium model
title_short Streptococcus pneumoniae infection increases expression of von Willebrand factor (vWF) in a mouse brain endothelium model
title_full Streptococcus pneumoniae infection increases expression of von Willebrand factor (vWF) in a mouse brain endothelium model
title_fullStr Streptococcus pneumoniae infection increases expression of von Willebrand factor (vWF) in a mouse brain endothelium model
title_full_unstemmed Streptococcus pneumoniae infection increases expression of von Willebrand factor (vWF) in a mouse brain endothelium model
title_sort streptococcus pneumoniae infection increases expression of von willebrand factor (vwf) in a mouse brain endothelium model
publisher Pusat Perubatan Universiti Kebangsaan Malaysia
publishDate 2018
url http://journalarticle.ukm.my/20206/1/13_ms0211_pdf_56811.pdf
http://journalarticle.ukm.my/20206/
https://www.medicineandhealthukm.com/toc/13/1
_version_ 1748181781479161856
score 13.211869

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