Streptococcus pneumoniae infection increases expression of von Willebrand factor (vWF) in a mouse brain endothelium model
von Willebrand factor (vWF) is a large multimeric glycoprotein which is normally released by the brain endothelial cells and is therefore present as a circulating protein in the blood. The initial and mature structure of vWF is uncleaved; however, pneumococcal infection can cause the cleavage of...
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| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
| Published: |
Pusat Perubatan Universiti Kebangsaan Malaysia
2018
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| Online Access: | http://journalarticle.ukm.my/20206/1/13_ms0211_pdf_56811.pdf http://journalarticle.ukm.my/20206/ https://www.medicineandhealthukm.com/toc/13/1 |
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| Summary: | von Willebrand factor (vWF) is a large multimeric glycoprotein which is normally
released by the brain endothelial cells and is therefore present as a circulating
protein in the blood. The initial and mature structure of vWF is uncleaved; however,
pneumococcal infection can cause the cleavage of the protein, thus formingseveral multimers at different sizes. The present study tested the hypothesis that
Streptococcus pneumoniae infection of brain endothelial cells in culture induces
the release of vWF at certain sizes time-dependently. Immortalized mouse brain
endothelial cells (bEnd.3) were infected at specific hours with Streptococcus
pneumoniae strains (serotype 19F). Immunofluorescence microscopy was
performed and quantified accordingly. Cells were also extracted, and the proteins
were separated into different sizes by western blot. The expression of vWF was
then analysed and compared for a potential relationship between the strains
and between the specific time of infection. The expression of vWF was elevated
during infection. Even though multiple and non-specific events can also cause the
cleavage of vWF, this study showed that the cleavage pattern of vWF is potentially
dependent on the incubation time of infection. |
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