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Microsatellite markers for the molecular characterisation of potentially commercial mango (Mangifera indica) progenies

Mango, or Mangifera indica as it is scientifically known, is a fruit crop that has significant importance to the global economy. This study characterised 10 commercially potential mango progenies using a total of 10 SSR markers. The analysis of the 10 SSR markers revealed the presence of 34 allele...

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Main Authors: Shahril Ab Razak,, Muhammad Afiq Tajol Ariffin,, Sharifah Munirah Syd Mohamad,, Nor Helwa Ezzah Nor Azman,, Mohd Azhar Hassan,, Johari Sarip,
Format: Article
Language:English
Published: Penerbit Universiti Kebangsaan Malaysia 2020
Online Access:http://journalarticle.ukm.my/16804/1/49_03_10.pdf
http://journalarticle.ukm.my/16804/
http://www.mabjournal.com/index.php?option=com_content&view=article&id=1039&catid=59:current-view&Itemid=56
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spelling my-ukm.journal.168042021-06-14T15:08:58Z http://journalarticle.ukm.my/16804/ Microsatellite markers for the molecular characterisation of potentially commercial mango (Mangifera indica) progenies Shahril Ab Razak, Muhammad Afiq Tajol Ariffin, Sharifah Munirah Syd Mohamad, Nor Helwa Ezzah Nor Azman, Mohd Azhar Hassan, Johari Sarip, Mango, or Mangifera indica as it is scientifically known, is a fruit crop that has significant importance to the global economy. This study characterised 10 commercially potential mango progenies using a total of 10 SSR markers. The analysis of the 10 SSR markers revealed the presence of 34 alleles. Each locus had 2–6 alleles equal to an average of 3.40 alleles per locus. The data also showed 0.115 (MiIIHR21) to 0.692 (MiIIHR28) polymorphic information content (PIC), equal to an average of 0.431. Each locus had heterozygosity and gene diversity values ranging from 0.044 (M1) to 0.957 (MiIIHR29) and from 0.122 (MiIIHR21) to 0.730 (MiIIHR29), respectively, averaging 0.480 and 0.476 each. The range of the pairwise genetic distance was 0.000 (ND014↔ND019) to 0.611 (ND020↔SB003). The dendrogram analysis showed that each mango progeny was successfully differentiated using the 10 SSR markers except for ND014 and ND019. The allele combination using these 10 SSR markers could be used to develop a DNA profile for fingerprinting purposes for each progeny except for ND014 and ND019, which can only be differentiated with the addition of an extra marker. The developed DNA fingerprinting profile could be used for cultivar identification and authentication. Additionally, breeders could also use the developed DNA profiles for plant variety protection (PVP) purposes. Penerbit Universiti Kebangsaan Malaysia 2020 Article PeerReviewed application/pdf en http://journalarticle.ukm.my/16804/1/49_03_10.pdf Shahril Ab Razak, and Muhammad Afiq Tajol Ariffin, and Sharifah Munirah Syd Mohamad, and Nor Helwa Ezzah Nor Azman, and Mohd Azhar Hassan, and Johari Sarip, (2020) Microsatellite markers for the molecular characterisation of potentially commercial mango (Mangifera indica) progenies. Malaysian Applied Biology, 49 (3). pp. 81-85. ISSN 0126-8643 http://www.mabjournal.com/index.php?option=com_content&view=article&id=1039&catid=59:current-view&Itemid=56
institution Universiti Kebangsaan Malaysia
building Tun Sri Lanang Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Kebangsaan Malaysia
content_source UKM Journal Article Repository
url_provider http://journalarticle.ukm.my/
language English
description Mango, or Mangifera indica as it is scientifically known, is a fruit crop that has significant importance to the global economy. This study characterised 10 commercially potential mango progenies using a total of 10 SSR markers. The analysis of the 10 SSR markers revealed the presence of 34 alleles. Each locus had 2–6 alleles equal to an average of 3.40 alleles per locus. The data also showed 0.115 (MiIIHR21) to 0.692 (MiIIHR28) polymorphic information content (PIC), equal to an average of 0.431. Each locus had heterozygosity and gene diversity values ranging from 0.044 (M1) to 0.957 (MiIIHR29) and from 0.122 (MiIIHR21) to 0.730 (MiIIHR29), respectively, averaging 0.480 and 0.476 each. The range of the pairwise genetic distance was 0.000 (ND014↔ND019) to 0.611 (ND020↔SB003). The dendrogram analysis showed that each mango progeny was successfully differentiated using the 10 SSR markers except for ND014 and ND019. The allele combination using these 10 SSR markers could be used to develop a DNA profile for fingerprinting purposes for each progeny except for ND014 and ND019, which can only be differentiated with the addition of an extra marker. The developed DNA fingerprinting profile could be used for cultivar identification and authentication. Additionally, breeders could also use the developed DNA profiles for plant variety protection (PVP) purposes.
format Article
author Shahril Ab Razak,
Muhammad Afiq Tajol Ariffin,
Sharifah Munirah Syd Mohamad,
Nor Helwa Ezzah Nor Azman,
Mohd Azhar Hassan,
Johari Sarip,
spellingShingle Shahril Ab Razak,
Muhammad Afiq Tajol Ariffin,
Sharifah Munirah Syd Mohamad,
Nor Helwa Ezzah Nor Azman,
Mohd Azhar Hassan,
Johari Sarip,
Microsatellite markers for the molecular characterisation of potentially commercial mango (Mangifera indica) progenies
author_facet Shahril Ab Razak,
Muhammad Afiq Tajol Ariffin,
Sharifah Munirah Syd Mohamad,
Nor Helwa Ezzah Nor Azman,
Mohd Azhar Hassan,
Johari Sarip,
author_sort Shahril Ab Razak,
title Microsatellite markers for the molecular characterisation of potentially commercial mango (Mangifera indica) progenies
title_short Microsatellite markers for the molecular characterisation of potentially commercial mango (Mangifera indica) progenies
title_full Microsatellite markers for the molecular characterisation of potentially commercial mango (Mangifera indica) progenies
title_fullStr Microsatellite markers for the molecular characterisation of potentially commercial mango (Mangifera indica) progenies
title_full_unstemmed Microsatellite markers for the molecular characterisation of potentially commercial mango (Mangifera indica) progenies
title_sort microsatellite markers for the molecular characterisation of potentially commercial mango (mangifera indica) progenies
publisher Penerbit Universiti Kebangsaan Malaysia
publishDate 2020
url http://journalarticle.ukm.my/16804/1/49_03_10.pdf
http://journalarticle.ukm.my/16804/
http://www.mabjournal.com/index.php?option=com_content&view=article&id=1039&catid=59:current-view&Itemid=56
_version_ 1703961592268324864
score 13.211869

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