Microsatellite markers for the molecular characterisation of potentially commercial mango (Mangifera indica) progenies
Mango, or Mangifera indica as it is scientifically known, is a fruit crop that has significant importance to the global economy. This study characterised 10 commercially potential mango progenies using a total of 10 SSR markers. The analysis of the 10 SSR markers revealed the presence of 34 allele...
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| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Penerbit Universiti Kebangsaan Malaysia
2020
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| Online Access: | http://journalarticle.ukm.my/16804/1/49_03_10.pdf http://journalarticle.ukm.my/16804/ http://www.mabjournal.com/index.php?option=com_content&view=article&id=1039&catid=59:current-view&Itemid=56 |
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| Summary: | Mango, or Mangifera indica as it is scientifically known, is a fruit crop that has significant importance to the global economy.
This study characterised 10 commercially potential mango progenies using a total of 10 SSR markers. The analysis of the 10
SSR markers revealed the presence of 34 alleles. Each locus had 2–6 alleles equal to an average of 3.40 alleles per locus. The
data also showed 0.115 (MiIIHR21) to 0.692 (MiIIHR28) polymorphic information content (PIC), equal to an average of
0.431. Each locus had heterozygosity and gene diversity values ranging from 0.044 (M1) to 0.957 (MiIIHR29) and from
0.122 (MiIIHR21) to 0.730 (MiIIHR29), respectively, averaging 0.480 and 0.476 each. The range of the pairwise genetic
distance was 0.000 (ND014↔ND019) to 0.611 (ND020↔SB003). The dendrogram analysis showed that each mango
progeny was successfully differentiated using the 10 SSR markers except for ND014 and ND019. The allele combination
using these 10 SSR markers could be used to develop a DNA profile for fingerprinting purposes for each progeny except for
ND014 and ND019, which can only be differentiated with the addition of an extra marker. The developed DNA fingerprinting
profile could be used for cultivar identification and authentication. Additionally, breeders could also use the developed DNA
profiles for plant variety protection (PVP) purposes. |
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