Cover Image

Effect of sulfur on nitrogen-containing plasma polymers in promoting osteogenic differentiation of Wharton’s jelly mesenchymal stem cells

The role of sulfur and its synergistic effects with nitrogen moieties in mediating stem cell proliferation and differentiation has become of interest to the tissue engineering community due to chemical similarities with the glycosaminoglycans found in human tissues and cells. Glycosaminoglycans...

Full description

Saved in:
Bibliographic Details
Main Authors: Siow, Kim Shyong, Arifah Rahman,, Amnani Aminuddin,, Ng, Pei Yuen
Format: Article
Language:English
Published: Penerbit Universiti Kebangsaan Malaysia 2021
Online Access:http://journalarticle.ukm.my/16408/1/23.pdf
http://journalarticle.ukm.my/16408/
https://www.ukm.my/jsm/malay_journals/jilid50bil1_2021/KandunganJilid50Bil1_2021.html
Tags: Add Tag
No Tags, Be the first to tag this record!
id my-ukm.journal.16408
record_format eprints
spelling my-ukm.journal.164082021-04-13T04:27:02Z http://journalarticle.ukm.my/16408/ Effect of sulfur on nitrogen-containing plasma polymers in promoting osteogenic differentiation of Wharton’s jelly mesenchymal stem cells Siow, Kim Shyong Arifah Rahman, Amnani Aminuddin, Ng, Pei Yuen The role of sulfur and its synergistic effects with nitrogen moieties in mediating stem cell proliferation and differentiation has become of interest to the tissue engineering community due to chemical similarities with the glycosaminoglycans found in human tissues and cells. Glycosaminoglycans are biomolecules known to influence stem cell differentiation, but the roles of sulfur with different oxidation states on nitrogen-containing polymers have not been fully understood nor investigated. In this study, we used the plasma polymerization of 1,7-octadiene (ppOD), n-heptylamine (ppHA), ppHA grafted with vinyl-sulfonate via Michael-type addition (ppHA-SO3 ), thiophene (ppT), and ppT with air plasma treatment (ppT-air) to produce controlled amounts of nitrogen and sulfur moieties having different oxidation states, as confirmed by x-ray photoelectron spectroscopy. Assays of the proliferation and osteogenic activities of Wharton’s jelly mesenchymal stem cells (WJ-MSCs) showed the highest activities for ppHA, followed by ppHA-SO3 , due to high percentages of amines/amides and the absence of SO3 moieties in ppHA. Other plasma polymers showed less proliferation and osteogenic differentiation than the positive control (glass substrate); however, WJ-MSCs grown on ppT-air with its high percentages of SO4 displayed cytoskeletons intensified with actin stress fiber, unlike the thiol-dominated ppT. Finally, the presence of methyl groups in ppOD severely limited WJ-MSCs proliferation and differentiation. Overall, these results confirm the beneficial effects of amine/amide groups on WJ-MSCs proliferation and osteogenic differentiation, but the combination of these groups with sulfur of various oxidation states failed to further enhance such cellular activities. Penerbit Universiti Kebangsaan Malaysia 2021-01 Article PeerReviewed application/pdf en http://journalarticle.ukm.my/16408/1/23.pdf Siow, Kim Shyong and Arifah Rahman, and Amnani Aminuddin, and Ng, Pei Yuen (2021) Effect of sulfur on nitrogen-containing plasma polymers in promoting osteogenic differentiation of Wharton’s jelly mesenchymal stem cells. Sains Malaysiana, 50 (1). pp. 239-251. ISSN 0126-6039 https://www.ukm.my/jsm/malay_journals/jilid50bil1_2021/KandunganJilid50Bil1_2021.html
institution Universiti Kebangsaan Malaysia
building Tun Sri Lanang Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Kebangsaan Malaysia
content_source UKM Journal Article Repository
url_provider http://journalarticle.ukm.my/
language English
description The role of sulfur and its synergistic effects with nitrogen moieties in mediating stem cell proliferation and differentiation has become of interest to the tissue engineering community due to chemical similarities with the glycosaminoglycans found in human tissues and cells. Glycosaminoglycans are biomolecules known to influence stem cell differentiation, but the roles of sulfur with different oxidation states on nitrogen-containing polymers have not been fully understood nor investigated. In this study, we used the plasma polymerization of 1,7-octadiene (ppOD), n-heptylamine (ppHA), ppHA grafted with vinyl-sulfonate via Michael-type addition (ppHA-SO3 ), thiophene (ppT), and ppT with air plasma treatment (ppT-air) to produce controlled amounts of nitrogen and sulfur moieties having different oxidation states, as confirmed by x-ray photoelectron spectroscopy. Assays of the proliferation and osteogenic activities of Wharton’s jelly mesenchymal stem cells (WJ-MSCs) showed the highest activities for ppHA, followed by ppHA-SO3 , due to high percentages of amines/amides and the absence of SO3 moieties in ppHA. Other plasma polymers showed less proliferation and osteogenic differentiation than the positive control (glass substrate); however, WJ-MSCs grown on ppT-air with its high percentages of SO4 displayed cytoskeletons intensified with actin stress fiber, unlike the thiol-dominated ppT. Finally, the presence of methyl groups in ppOD severely limited WJ-MSCs proliferation and differentiation. Overall, these results confirm the beneficial effects of amine/amide groups on WJ-MSCs proliferation and osteogenic differentiation, but the combination of these groups with sulfur of various oxidation states failed to further enhance such cellular activities.
format Article
author Siow, Kim Shyong
Arifah Rahman,
Amnani Aminuddin,
Ng, Pei Yuen
spellingShingle Siow, Kim Shyong
Arifah Rahman,
Amnani Aminuddin,
Ng, Pei Yuen
Effect of sulfur on nitrogen-containing plasma polymers in promoting osteogenic differentiation of Wharton’s jelly mesenchymal stem cells
author_facet Siow, Kim Shyong
Arifah Rahman,
Amnani Aminuddin,
Ng, Pei Yuen
author_sort Siow, Kim Shyong
title Effect of sulfur on nitrogen-containing plasma polymers in promoting osteogenic differentiation of Wharton’s jelly mesenchymal stem cells
title_short Effect of sulfur on nitrogen-containing plasma polymers in promoting osteogenic differentiation of Wharton’s jelly mesenchymal stem cells
title_full Effect of sulfur on nitrogen-containing plasma polymers in promoting osteogenic differentiation of Wharton’s jelly mesenchymal stem cells
title_fullStr Effect of sulfur on nitrogen-containing plasma polymers in promoting osteogenic differentiation of Wharton’s jelly mesenchymal stem cells
title_full_unstemmed Effect of sulfur on nitrogen-containing plasma polymers in promoting osteogenic differentiation of Wharton’s jelly mesenchymal stem cells
title_sort effect of sulfur on nitrogen-containing plasma polymers in promoting osteogenic differentiation of wharton’s jelly mesenchymal stem cells
publisher Penerbit Universiti Kebangsaan Malaysia
publishDate 2021
url http://journalarticle.ukm.my/16408/1/23.pdf
http://journalarticle.ukm.my/16408/
https://www.ukm.my/jsm/malay_journals/jilid50bil1_2021/KandunganJilid50Bil1_2021.html
_version_ 1696977215335432192
score 13.211869

Similar Items