Effect of sulfur on nitrogen-containing plasma polymers in promoting osteogenic differentiation of Wharton’s jelly mesenchymal stem cells
The role of sulfur and its synergistic effects with nitrogen moieties in mediating stem cell proliferation and differentiation has become of interest to the tissue engineering community due to chemical similarities with the glycosaminoglycans found in human tissues and cells. Glycosaminoglycans...
Saved in:
| Main Authors: | , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Penerbit Universiti Kebangsaan Malaysia
2021
|
| Online Access: | http://journalarticle.ukm.my/16408/1/23.pdf http://journalarticle.ukm.my/16408/ https://www.ukm.my/jsm/malay_journals/jilid50bil1_2021/KandunganJilid50Bil1_2021.html |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| Summary: | The role of sulfur and its synergistic effects with nitrogen moieties in mediating stem cell proliferation and
differentiation has become of interest to the tissue engineering community due to chemical similarities with the
glycosaminoglycans found in human tissues and cells. Glycosaminoglycans are biomolecules known to influence stem
cell differentiation, but the roles of sulfur with different oxidation states on nitrogen-containing polymers have not
been fully understood nor investigated. In this study, we used the plasma polymerization of 1,7-octadiene (ppOD),
n-heptylamine (ppHA), ppHA grafted with vinyl-sulfonate via Michael-type addition (ppHA-SO3
), thiophene (ppT),
and ppT with air plasma treatment (ppT-air) to produce controlled amounts of nitrogen and sulfur moieties having
different oxidation states, as confirmed by x-ray photoelectron spectroscopy. Assays of the proliferation and osteogenic
activities of Wharton’s jelly mesenchymal stem cells (WJ-MSCs) showed the highest activities for ppHA, followed by
ppHA-SO3
, due to high percentages of amines/amides and the absence of SO3
moieties in ppHA. Other plasma polymers
showed less proliferation and osteogenic differentiation than the positive control (glass substrate); however, WJ-MSCs
grown on ppT-air with its high percentages of SO4
displayed cytoskeletons intensified with actin stress fiber, unlike the
thiol-dominated ppT. Finally, the presence of methyl groups in ppOD severely limited WJ-MSCs proliferation and
differentiation. Overall, these results confirm the beneficial effects of amine/amide groups on WJ-MSCs proliferation
and osteogenic differentiation, but the combination of these groups with sulfur of various oxidation states failed to
further enhance such cellular activities. |
|---|