Cover Image

Essential oil constituents assessment and antioxidant profiles of Stevia rebaudiana Bertoni leaves from Malaysia

Since the 15th century, essential oils (EO) were having a strong interest as therapeutic agents and recently EO has been widely used in pharmaceutical, cosmetic, food and agricultural industries. The previous study showed that EOs from Stevia rebaudiana Bertoni (SrB) possess high antioxidant, anti...

Full description

Saved in:
Bibliographic Details
Main Authors: Nur Izzati M.Z.,, Uswatun Hasanah Z.,, Siti Salwa A.G.,, Suhaili S.,, Mohd. Izuan Effendi H.,
Format: Article
Language:English
Published: Penerbit Universiti Kebangsaan Malaysia 2019
Online Access:http://journalarticle.ukm.my/14666/1/48_02_08.pdf
http://journalarticle.ukm.my/14666/
http://www.mabjournal.com/index.php?option=com_content&view=article&id=921&catid=59:current-view&Itemid=56
Tags: Add Tag
No Tags, Be the first to tag this record!
id my-ukm.journal.14666
record_format eprints
spelling my-ukm.journal.146662020-05-21T14:03:42Z http://journalarticle.ukm.my/14666/ Essential oil constituents assessment and antioxidant profiles of Stevia rebaudiana Bertoni leaves from Malaysia Nur Izzati M.Z., Uswatun Hasanah Z., Siti Salwa A.G., Suhaili S., Mohd. Izuan Effendi H., Since the 15th century, essential oils (EO) were having a strong interest as therapeutic agents and recently EO has been widely used in pharmaceutical, cosmetic, food and agricultural industries. The previous study showed that EOs from Stevia rebaudiana Bertoni (SrB) possess high antioxidant, anti-inflammatory, and antimicrobial properties. In this study, the chemical constituents of EO from SrB leaves which grown in Selangor, Malaysia were obtained by ethanol Soxhlet extraction. Total polyphenolic content (TPC), total flavonoid content (TFC), and chemical composition using a gas chromatography-mass selective detector (GC-MSD) analyses of Stevia rebaudiana Bertoni essential oil (SrB EO) were carried out. The TPC and TFC of SrB EO were 14.44±0.63 mg gallic acid equivalent (GAE) /g sample extract and 32.19±1.38 mg quercetin equivalent (QE)/g sample extract, respectively. Based on GC-MSD analyses, one hundred peaks were obtained and 28 compounds were identified where lupeyl acetate occupied the highest percentage with 14.25% of the chemical constituents. The antioxidant activities of SrB EO were studied using 2,2-diphenyl-1-picrylhydrazyl (DPPH), 2,2-azino-bis(3-ethylbenzothiazoline-6- sulphonic acid (ABTS), and ferric reducing antioxidant power (FRAP) assays. The IC50 of SrB EO using DPPH and ABTS were 71.36±1.57 µg/mL Trolox equivalent (TE) and 8.88×102±0.16 µg/mL TE, respectively while the FRAP activity was 9.55±0.26 mg/g TE. These results show the promising antioxidant properties of SrB EO. Penerbit Universiti Kebangsaan Malaysia 2019-05 Article PeerReviewed application/pdf en http://journalarticle.ukm.my/14666/1/48_02_08.pdf Nur Izzati M.Z., and Uswatun Hasanah Z., and Siti Salwa A.G., and Suhaili S., and Mohd. Izuan Effendi H., (2019) Essential oil constituents assessment and antioxidant profiles of Stevia rebaudiana Bertoni leaves from Malaysia. Malaysian Applied Biology, 48 (2). pp. 55-62. ISSN 0126-8643 http://www.mabjournal.com/index.php?option=com_content&view=article&id=921&catid=59:current-view&Itemid=56
institution Universiti Kebangsaan Malaysia
building Tun Sri Lanang Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Kebangsaan Malaysia
content_source UKM Journal Article Repository
url_provider http://journalarticle.ukm.my/
language English
description Since the 15th century, essential oils (EO) were having a strong interest as therapeutic agents and recently EO has been widely used in pharmaceutical, cosmetic, food and agricultural industries. The previous study showed that EOs from Stevia rebaudiana Bertoni (SrB) possess high antioxidant, anti-inflammatory, and antimicrobial properties. In this study, the chemical constituents of EO from SrB leaves which grown in Selangor, Malaysia were obtained by ethanol Soxhlet extraction. Total polyphenolic content (TPC), total flavonoid content (TFC), and chemical composition using a gas chromatography-mass selective detector (GC-MSD) analyses of Stevia rebaudiana Bertoni essential oil (SrB EO) were carried out. The TPC and TFC of SrB EO were 14.44±0.63 mg gallic acid equivalent (GAE) /g sample extract and 32.19±1.38 mg quercetin equivalent (QE)/g sample extract, respectively. Based on GC-MSD analyses, one hundred peaks were obtained and 28 compounds were identified where lupeyl acetate occupied the highest percentage with 14.25% of the chemical constituents. The antioxidant activities of SrB EO were studied using 2,2-diphenyl-1-picrylhydrazyl (DPPH), 2,2-azino-bis(3-ethylbenzothiazoline-6- sulphonic acid (ABTS), and ferric reducing antioxidant power (FRAP) assays. The IC50 of SrB EO using DPPH and ABTS were 71.36±1.57 µg/mL Trolox equivalent (TE) and 8.88×102±0.16 µg/mL TE, respectively while the FRAP activity was 9.55±0.26 mg/g TE. These results show the promising antioxidant properties of SrB EO.
format Article
author Nur Izzati M.Z.,
Uswatun Hasanah Z.,
Siti Salwa A.G.,
Suhaili S.,
Mohd. Izuan Effendi H.,
spellingShingle Nur Izzati M.Z.,
Uswatun Hasanah Z.,
Siti Salwa A.G.,
Suhaili S.,
Mohd. Izuan Effendi H.,
Essential oil constituents assessment and antioxidant profiles of Stevia rebaudiana Bertoni leaves from Malaysia
author_facet Nur Izzati M.Z.,
Uswatun Hasanah Z.,
Siti Salwa A.G.,
Suhaili S.,
Mohd. Izuan Effendi H.,
author_sort Nur Izzati M.Z.,
title Essential oil constituents assessment and antioxidant profiles of Stevia rebaudiana Bertoni leaves from Malaysia
title_short Essential oil constituents assessment and antioxidant profiles of Stevia rebaudiana Bertoni leaves from Malaysia
title_full Essential oil constituents assessment and antioxidant profiles of Stevia rebaudiana Bertoni leaves from Malaysia
title_fullStr Essential oil constituents assessment and antioxidant profiles of Stevia rebaudiana Bertoni leaves from Malaysia
title_full_unstemmed Essential oil constituents assessment and antioxidant profiles of Stevia rebaudiana Bertoni leaves from Malaysia
title_sort essential oil constituents assessment and antioxidant profiles of stevia rebaudiana bertoni leaves from malaysia
publisher Penerbit Universiti Kebangsaan Malaysia
publishDate 2019
url http://journalarticle.ukm.my/14666/1/48_02_08.pdf
http://journalarticle.ukm.my/14666/
http://www.mabjournal.com/index.php?option=com_content&view=article&id=921&catid=59:current-view&Itemid=56
_version_ 1669008495653945344
score 13.211869

Similar Items