Essential oil constituents assessment and antioxidant profiles of Stevia rebaudiana Bertoni leaves from Malaysia
Since the 15th century, essential oils (EO) were having a strong interest as therapeutic agents and recently EO has been widely used in pharmaceutical, cosmetic, food and agricultural industries. The previous study showed that EOs from Stevia rebaudiana Bertoni (SrB) possess high antioxidant, anti...
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| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Penerbit Universiti Kebangsaan Malaysia
2019
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| Online Access: | http://journalarticle.ukm.my/14666/1/48_02_08.pdf http://journalarticle.ukm.my/14666/ http://www.mabjournal.com/index.php?option=com_content&view=article&id=921&catid=59:current-view&Itemid=56 |
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| Summary: | Since the 15th century, essential oils (EO) were having a strong interest as therapeutic agents and recently EO has been
widely used in pharmaceutical, cosmetic, food and agricultural industries. The previous study showed that EOs from Stevia
rebaudiana Bertoni (SrB) possess high antioxidant, anti-inflammatory, and antimicrobial properties. In this study, the chemical
constituents of EO from SrB leaves which grown in Selangor, Malaysia were obtained by ethanol Soxhlet extraction. Total
polyphenolic content (TPC), total flavonoid content (TFC), and chemical composition using a gas chromatography-mass
selective detector (GC-MSD) analyses of Stevia rebaudiana Bertoni essential oil (SrB EO) were carried out. The TPC and
TFC of SrB EO were 14.44±0.63 mg gallic acid equivalent (GAE) /g sample extract and 32.19±1.38 mg quercetin equivalent
(QE)/g sample extract, respectively. Based on GC-MSD analyses, one hundred peaks were obtained and 28 compounds
were identified where lupeyl acetate occupied the highest percentage with 14.25% of the chemical constituents. The antioxidant
activities of SrB EO were studied using 2,2-diphenyl-1-picrylhydrazyl (DPPH), 2,2-azino-bis(3-ethylbenzothiazoline-6-
sulphonic acid (ABTS), and ferric reducing antioxidant power (FRAP) assays. The IC50 of SrB EO using DPPH and ABTS
were 71.36±1.57 µg/mL Trolox equivalent (TE) and 8.88×102±0.16 µg/mL TE, respectively while the FRAP activity was
9.55±0.26 mg/g TE. These results show the promising antioxidant properties of SrB EO. |
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