Active compound, antioxidant, antiproliferative and effect on STZ induced zebrafish of various crude extracts from Boletus qriseipurpureus
Boletus qriseipurpureus (gelam mushroom) is a mushroom used by locals in Tok Bali, Kelantan, Malaysia to treat diabetes, cervical cancer and breast cancer. The active compounds in B. qriseipurpureus remain unidentified. Therefore, in this study, we investigated the potential medicinal properties o...
Saved in:
| Main Authors: | , , , , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Malaysian Society of Applied Biology
2016
|
| Online Access: | http://journalarticle.ukm.my/11801/1/45_1_08.pdf http://journalarticle.ukm.my/11801/ http://www.mabjournal.com/index.php?option=com_content&view=article&id=555&catid=59:current-view&Itemid=56 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| id |
my-ukm.journal.11801 |
|---|---|
| record_format |
eprints |
| spelling |
my-ukm.journal.118012018-06-29T08:08:59Z http://journalarticle.ukm.my/11801/ Active compound, antioxidant, antiproliferative and effect on STZ induced zebrafish of various crude extracts from Boletus qriseipurpureus Sutha Muniandy, Fauzi Daud, Sahidan Senafi, Mahanem Mat Noor, Malina Kumaran, Andi Norafiqah Andik Mohd Alwi, Ayumawarni Yusni Yeng Jee Long, Rahimah Mohamad Zuki, Janet Salomi Isac, Subhashini Krishnan, Deeviya Gobalan, Lim, Yi Chieh Azwan Mat Lazim, Shazrul Fazry, Boletus qriseipurpureus (gelam mushroom) is a mushroom used by locals in Tok Bali, Kelantan, Malaysia to treat diabetes, cervical cancer and breast cancer. The active compounds in B. qriseipurpureus remain unidentified. Therefore, in this study, we investigated the potential medicinal properties of B. qriseipurpureus extracts (hot water, cold water and methanol extracts) by conducting preliminary phytochemical screening, in vitro antioxidant, in vitro antiproliferative and in vivo antidiabetic test. Biochemical assays were performed to detect the presence of alkaloids, anthraquinones, flavonoids, reducing sugars, saponins, steroids and tannins in B. qriseipurpureus extracts. 1,1-diphenyl-2-picrylhydrazyl (DPPH) was used to evaluate the antioxidant capacity of B. qriseipurpureus extracts, while MTT cell proliferation assay (3-(4,5-dimethylthiazolyl-2)-2,5-diphenyltetrazolium bromide) was used to investigate the extracts antiproliferative activity. The effects of B. qriseipurpureus extracts on streptozotocin (STZ) induced zebrafish were examined at the concentration of 45 mg/mL, 90 mg/mL and 135 mg/mL. The effect of extracts were measured by the regenerative growth rate of the amputated caudal fin for fourteen days post transection. Screening of the mushroom extracts for active compounds revealed the presence of alkaloids, flavonoids, saponins and tannins in all test extracts. Reducing sugars and anthraquinones are only detected in hot water and cold water of B. qriseipurpureus extracts. The half maximal inhibitory concentration (IC50) of DPPH by hot water, cold water and methanol extract of B. qriseipurpureus are 1.79 mg/mL, 1.97 mg/mL and 3.98 mg/mL respectively. The MTT assay indicated that all extracts exhibited significant antiproliferative effects on MCF-7 cell line after 72 hours with IC50 of 7.7 mg/mL for hot water extract, 8.2 mg/ mL for cold water extract and 16.1 mg/mL for methanol extract but do not display any significant cytotoxic effect. The STZ induced zebrafish treated with 135 mg/mL of hot water B. qriseipurpureus extract for 14 days showed the highest regeneration growth rate of caudal fin (5.04 ± 0.43%) compared to fish treated with metformin (5.72 ± 0.64 %). In this study, we showed the potential of hot water B. qriseipurpureus extracts as a potent therapeutic agent for diabetes and as an alternative natural source of antioxidant. Malaysian Society of Applied Biology 2016-06 Article PeerReviewed application/pdf en http://journalarticle.ukm.my/11801/1/45_1_08.pdf Sutha Muniandy, and Fauzi Daud, and Sahidan Senafi, and Mahanem Mat Noor, and Malina Kumaran, and Andi Norafiqah Andik Mohd Alwi, and Ayumawarni Yusni Yeng Jee Long, and Rahimah Mohamad Zuki, and Janet Salomi Isac, and Subhashini Krishnan, and Deeviya Gobalan, and Lim, Yi Chieh and Azwan Mat Lazim, and Shazrul Fazry, (2016) Active compound, antioxidant, antiproliferative and effect on STZ induced zebrafish of various crude extracts from Boletus qriseipurpureus. Malaysian Applied Biology, 45 (1). pp. 69-80. ISSN 0126-8643 http://www.mabjournal.com/index.php?option=com_content&view=article&id=555&catid=59:current-view&Itemid=56 |
| institution |
Universiti Kebangsaan Malaysia |
| building |
Perpustakaan Tun Sri Lanang Library |
| collection |
Institutional Repository |
| continent |
Asia |
| country |
Malaysia |
| content_provider |
Universiti Kebangsaan Malaysia |
| content_source |
UKM Journal Article Repository |
| url_provider |
http://journalarticle.ukm.my/ |
| language |
English |
| description |
Boletus qriseipurpureus (gelam mushroom) is a mushroom used by locals in Tok Bali, Kelantan, Malaysia to treat diabetes,
cervical cancer and breast cancer. The active compounds in B. qriseipurpureus remain unidentified. Therefore, in this study,
we investigated the potential medicinal properties of B. qriseipurpureus extracts (hot water, cold water and methanol extracts)
by conducting preliminary phytochemical screening, in vitro antioxidant, in vitro antiproliferative and in vivo antidiabetic test.
Biochemical assays were performed to detect the presence of alkaloids, anthraquinones, flavonoids, reducing sugars, saponins,
steroids and tannins in B. qriseipurpureus extracts. 1,1-diphenyl-2-picrylhydrazyl (DPPH) was used to evaluate the antioxidant
capacity of B. qriseipurpureus extracts, while MTT cell proliferation assay (3-(4,5-dimethylthiazolyl-2)-2,5-diphenyltetrazolium
bromide) was used to investigate the extracts antiproliferative activity. The effects of B. qriseipurpureus extracts on
streptozotocin (STZ) induced zebrafish were examined at the concentration of 45 mg/mL, 90 mg/mL and 135 mg/mL. The
effect of extracts were measured by the regenerative growth rate of the amputated caudal fin for fourteen days post transection.
Screening of the mushroom extracts for active compounds revealed the presence of alkaloids, flavonoids, saponins and tannins
in all test extracts. Reducing sugars and anthraquinones are only detected in hot water and cold water of B. qriseipurpureus
extracts. The half maximal inhibitory concentration (IC50) of DPPH by hot water, cold water and methanol extract of B.
qriseipurpureus are 1.79 mg/mL, 1.97 mg/mL and 3.98 mg/mL respectively. The MTT assay indicated that all extracts exhibited
significant antiproliferative effects on MCF-7 cell line after 72 hours with IC50 of 7.7 mg/mL for hot water extract, 8.2 mg/
mL for cold water extract and 16.1 mg/mL for methanol extract but do not display any significant cytotoxic effect. The STZ
induced zebrafish treated with 135 mg/mL of hot water B. qriseipurpureus extract for 14 days showed the highest regeneration
growth rate of caudal fin (5.04 ± 0.43%) compared to fish treated with metformin (5.72 ± 0.64 %). In this study, we showed
the potential of hot water B. qriseipurpureus extracts as a potent therapeutic agent for diabetes and as an alternative natural
source of antioxidant. |
| format |
Article |
| author |
Sutha Muniandy, Fauzi Daud, Sahidan Senafi, Mahanem Mat Noor, Malina Kumaran, Andi Norafiqah Andik Mohd Alwi, Ayumawarni Yusni Yeng Jee Long, Rahimah Mohamad Zuki, Janet Salomi Isac, Subhashini Krishnan, Deeviya Gobalan, Lim, Yi Chieh Azwan Mat Lazim, Shazrul Fazry, |
| spellingShingle |
Sutha Muniandy, Fauzi Daud, Sahidan Senafi, Mahanem Mat Noor, Malina Kumaran, Andi Norafiqah Andik Mohd Alwi, Ayumawarni Yusni Yeng Jee Long, Rahimah Mohamad Zuki, Janet Salomi Isac, Subhashini Krishnan, Deeviya Gobalan, Lim, Yi Chieh Azwan Mat Lazim, Shazrul Fazry, Active compound, antioxidant, antiproliferative and effect on STZ induced zebrafish of various crude extracts from Boletus qriseipurpureus |
| author_facet |
Sutha Muniandy, Fauzi Daud, Sahidan Senafi, Mahanem Mat Noor, Malina Kumaran, Andi Norafiqah Andik Mohd Alwi, Ayumawarni Yusni Yeng Jee Long, Rahimah Mohamad Zuki, Janet Salomi Isac, Subhashini Krishnan, Deeviya Gobalan, Lim, Yi Chieh Azwan Mat Lazim, Shazrul Fazry, |
| author_sort |
Sutha Muniandy, |
| title |
Active compound, antioxidant, antiproliferative and effect on STZ induced zebrafish of various crude extracts from Boletus qriseipurpureus |
| title_short |
Active compound, antioxidant, antiproliferative and effect on STZ induced zebrafish of various crude extracts from Boletus qriseipurpureus |
| title_full |
Active compound, antioxidant, antiproliferative and effect on STZ induced zebrafish of various crude extracts from Boletus qriseipurpureus |
| title_fullStr |
Active compound, antioxidant, antiproliferative and effect on STZ induced zebrafish of various crude extracts from Boletus qriseipurpureus |
| title_full_unstemmed |
Active compound, antioxidant, antiproliferative and effect on STZ induced zebrafish of various crude extracts from Boletus qriseipurpureus |
| title_sort |
active compound, antioxidant, antiproliferative and effect on stz induced zebrafish of various crude extracts from boletus qriseipurpureus |
| publisher |
Malaysian Society of Applied Biology |
| publishDate |
2016 |
| url |
http://journalarticle.ukm.my/11801/1/45_1_08.pdf http://journalarticle.ukm.my/11801/ http://www.mabjournal.com/index.php?option=com_content&view=article&id=555&catid=59:current-view&Itemid=56 |
| _version_ |
1643738606462828544 |
| score |
13.211869 |