Active compound, antioxidant, antiproliferative and effect on STZ induced zebrafish of various crude extracts from Boletus qriseipurpureus
Boletus qriseipurpureus (gelam mushroom) is a mushroom used by locals in Tok Bali, Kelantan, Malaysia to treat diabetes, cervical cancer and breast cancer. The active compounds in B. qriseipurpureus remain unidentified. Therefore, in this study, we investigated the potential medicinal properties o...
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| Main Authors: | , , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Malaysian Society of Applied Biology
2016
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| Online Access: | http://journalarticle.ukm.my/11801/1/45_1_08.pdf http://journalarticle.ukm.my/11801/ http://www.mabjournal.com/index.php?option=com_content&view=article&id=555&catid=59:current-view&Itemid=56 |
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| Summary: | Boletus qriseipurpureus (gelam mushroom) is a mushroom used by locals in Tok Bali, Kelantan, Malaysia to treat diabetes,
cervical cancer and breast cancer. The active compounds in B. qriseipurpureus remain unidentified. Therefore, in this study,
we investigated the potential medicinal properties of B. qriseipurpureus extracts (hot water, cold water and methanol extracts)
by conducting preliminary phytochemical screening, in vitro antioxidant, in vitro antiproliferative and in vivo antidiabetic test.
Biochemical assays were performed to detect the presence of alkaloids, anthraquinones, flavonoids, reducing sugars, saponins,
steroids and tannins in B. qriseipurpureus extracts. 1,1-diphenyl-2-picrylhydrazyl (DPPH) was used to evaluate the antioxidant
capacity of B. qriseipurpureus extracts, while MTT cell proliferation assay (3-(4,5-dimethylthiazolyl-2)-2,5-diphenyltetrazolium
bromide) was used to investigate the extracts antiproliferative activity. The effects of B. qriseipurpureus extracts on
streptozotocin (STZ) induced zebrafish were examined at the concentration of 45 mg/mL, 90 mg/mL and 135 mg/mL. The
effect of extracts were measured by the regenerative growth rate of the amputated caudal fin for fourteen days post transection.
Screening of the mushroom extracts for active compounds revealed the presence of alkaloids, flavonoids, saponins and tannins
in all test extracts. Reducing sugars and anthraquinones are only detected in hot water and cold water of B. qriseipurpureus
extracts. The half maximal inhibitory concentration (IC50) of DPPH by hot water, cold water and methanol extract of B.
qriseipurpureus are 1.79 mg/mL, 1.97 mg/mL and 3.98 mg/mL respectively. The MTT assay indicated that all extracts exhibited
significant antiproliferative effects on MCF-7 cell line after 72 hours with IC50 of 7.7 mg/mL for hot water extract, 8.2 mg/
mL for cold water extract and 16.1 mg/mL for methanol extract but do not display any significant cytotoxic effect. The STZ
induced zebrafish treated with 135 mg/mL of hot water B. qriseipurpureus extract for 14 days showed the highest regeneration
growth rate of caudal fin (5.04 ± 0.43%) compared to fish treated with metformin (5.72 ± 0.64 %). In this study, we showed
the potential of hot water B. qriseipurpureus extracts as a potent therapeutic agent for diabetes and as an alternative natural
source of antioxidant. |
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