Cloning, expression, and purification of the hemolysin/cytolysin (HlyE antigen) from Salmonella enterica serovar Typhi: Potential application for immunoassay development

The hemolysin (HlyE) protein of Salmonella enterica serovar Typhi was reported to be antigenic. This work describes the cloning, expression, and purification of a hexahistidine-tagged HlyE protein under native conditions. Immunoblot analysis and a competitive enzyme-linked immunosorbent assay using...

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Bibliographic Details

Main Authors:	Ong E.B.B., Anthony A.A., Ismail A., Lim T.S.
Other Authors:	Chancellory Building
Format:	Article
Language:	English en_US
Published:	Elsevier Inc. 2024
Subjects:	Antigen HlyE Immunoblot, ELISA Salmonella Typhi
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