Widefield Two-photon Excitation Without Scanning: Live Cell Microscopy With Hightime Resolution And Low Photo-bleaching

We demonstrate fluorescence imaging by two-photon excitation without scanning in biological specimens as previously described by Hwang and co-workers, but with an increased field size and with framing rates of up to 100 Hz. During recordings of synaptically-driven Ca2+ events in primary rat hippocam...

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Bibliographic Details

Main Authors:	Nor Zaihana Abdul Rahman, Rumelo Amor, Alison McDonald, Johanna Trägårdh, Gillian Robb, Louise Wilson, John Dempster, William Bradshaw Amos1, Trevor J. Bushell, Gail McConnell
Format:	Article
Language:	en_US
Published:	Amor et al 2024
Subjects:	Fluorescence imaging, Lasers, Optical lenses, Specimen sectioning,Two-photon excitation microscopy, Fluorescence, Fluorescence microscopy, Imaging techniques
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