Multiple overlap extension PCR (MOE-PCR): an effective technical shortcut to high throughput synthetic biology
The current study describes multiple-overlap-extension PCR (MOE-PCR) as a simple and effective approach to assembling multiple DNA fragments with various sizes and features in a single in vitro reaction. In this research, 50 bp of homology in overlapping DNA fragments and a specific touchdown PCR pr...
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| Main Authors: | , , , , , , |
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| Format: | Article |
| Published: |
Royal Society of Chemistry
2016
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| Online Access: | http://psasir.upm.edu.my/id/eprint/53351/ http://pubs.rsc.org/en/content/articlelanding/2016/ra/c6ra13172g#!divAbstract |
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