In silico design of a chimeric aspartic protease for cheese production using parental sequences from 2ASI and 1MPP

Aspartic proteases from Rhizomucor miehei (RMP; PDB 2ASI) and Mucor pusillus (MPP; PDB 1MPP) are widely used in cheese production for milk coagulation, but their high thermostability and nonspecific proteolytic activity can cause over-hydrolysis of casein, resulting in bitterness and reduced yields....

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Main Authors:	Saleem, Shilan S., Abdul Rahman, Mohd Basyaruddin, Leow, Adam Thean Chor, Muhd Noor, Noor Dina, Salleh, Abu Bakar, Oslan, Siti Nurbaya
Format:	Article
Language:	en
Published:	Elsevier B.V. 2026
Subjects:	Food Science Structural Biology
Online Access:	http://psasir.upm.edu.my/id/eprint/123040/1/123040.pdf http://psasir.upm.edu.my/id/eprint/123040/ https://www.sciencedirect.com/science/article/pii/S0141813026001662
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