Designing novel aspartic protease (NAP) using ancestral sequence reconstruction (ASR) for cheesemaking

Designing novel aspartic protease (NAP) using ancestral sequence reconstruction (ASR) for cheesemaking

Rhizomucor miehei protease (RMP), a chymosin-like fungal enzyme, is among the most widely used non-animal milk coagulant enzymes (MCEs) in cheese production. However, like other current MCEs, RMP exhibits non-specific proteolytic activity and high thermostability, leading to excessive casein degrada...

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Bibliographic Details
Main Authors: Saleem, Shilan S., Akinola, Oluwasola Michael, Abdul Rahman, Mohd Basyaruddin, Leow, Adam Thean Chor, Muhd Noor, Noor Dina, Salleh, Abu Bakar, Oslan, Siti Nurbaya
Format: Article
Language:en
Published: Springer 2025
Subjects:
Biotechnology
Bioengineering
Online Access:http://psasir.upm.edu.my/id/eprint/122307/1/122307.pdf
http://psasir.upm.edu.my/id/eprint/122307/
https://link.springer.com/article/10.1007/s12033-025-01470-0?error=cookies_not_supported&code=91c2b516-3b0c-46a3-bdc1-86b49c5e3a81
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http://psasir.upm.edu.my/id/eprint/122307/1/122307.pdf
http://psasir.upm.edu.my/id/eprint/122307/
https://link.springer.com/article/10.1007/s12033-025-01470-0?error=cookies_not_supported&code=91c2b516-3b0c-46a3-bdc1-86b49c5e3a81

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