Long-read sequencing and genome assembly of natural history collection samples and challenging specimens

Museum collections harbor millions of samples, largely unutilized for long-read sequencing. Here, we use ethanol-preserved samples containing kilobase-sized DNA to show that amplification-free protocols can yield contiguous genome assemblies. Additionally, using a modified amplification-based protoc...

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Bibliographic Details

Main Authors:	Bein, Bernhard, Chrysostomakis, Ioannis, Arantes, Larissa S., Brown, Tom, Gerheim, Charlotte, Schell, Tilman, Schneider, Clément, Leushkin, Evgeny, Chen, Zeyuan, Sigwart, Julia, Gonzalez, Vanessa, Wong, Nur Leena W.S., Santos, Fabricio R., Blom, Mozes P.K., Mayer, Frieder, Mazzoni, Camila J., Böhne, Astrid, Winkler, Sylke, Greve, Carola, Hiller, Michael
Format:	Article
Language:	en
Published:	BioMed Central 2025
Online Access:	http://psasir.upm.edu.my/id/eprint/120192/1/120192.pdf http://psasir.upm.edu.my/id/eprint/120192/ https://genomebiology.biomedcentral.com/articles/10.1186/s13059-025-03487-9
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