Serum from Nipah virus patients recognises recombinant viral proteins produced in Escherichia coli
The genes for Nipah virus (NiV) proteins were amplified from viral RNA, cloned into the plasmid pTriEx-3 Hygro, expressed, and purified using immobilized metal affinity chromatography. The recombinant N, F, and G NiV proteins (rNiV-N, rNiV-F, and rNiV-G), were successfully expressed in Escherichia c...
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| Main Authors: | , , , , |
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| Format: | Article |
| Published: |
Institute of Infectious Diseases
2017
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| Subjects: | |
| Online Access: | http://eprints.um.edu.my/22882/ https://doi.org/10.7883/yoken.JJID.2015.501 |
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