Optimised conditions for pulsed field gel electrophoresis (PFGE) separations of Pasteurella multocida B:2 DNA
Pulsed field conditions specific for Pasteurella multocida B:2 have been optimised to obtain a good separation resolution. Restriction enzyme, NotI, was the most suitable as it generates discernible number of bands. Other optimised electrophoretic conditions were: 1.5% agarose gel concentration, 200...
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| Main Authors: | , , , |
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| Format: | Article |
| Language: | en |
| Published: |
Malaysian Society for Molecular Biology and Biotechnology
2001
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| Subjects: | |
| Online Access: | http://eprints.um.edu.my/18592/1/APJMBB2001PFGEPasteurella.pdf http://eprints.um.edu.my/18592/ http://www.msmbb.my/index.php/publication |
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| Summary: | Pulsed field conditions specific for Pasteurella multocida B:2 have been optimised to obtain a good separation resolution. Restriction enzyme, NotI, was the most suitable as it generates discernible number of bands. Other optimised electrophoretic conditions were: 1.5% agarose gel concentration, 200 V, ramped pulsed time of 1-25 s and a run time of 26 h. This research is important for fast and accurate characterisation of this bacterial strain following an outbreak in Malaysia. |
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