Optimised conditions for pulsed field gel electrophoresis (PFGE) separations of Pasteurella multocida B:2 DNA

Pulsed field conditions specific for Pasteurella multocida B:2 have been optimised to obtain a good separation resolution. Restriction enzyme, NotI, was the most suitable as it generates discernible number of bands. Other optimised electrophoretic conditions were: 1.5% agarose gel concentration, 200...

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Bibliographic Details

Main Authors:	Hasima, N.N., Saidi, J., Thong, K.L., Ansary, A.
Format:	Article
Language:	en
Published:	Malaysian Society for Molecular Biology and Biotechnology 2001
Subjects:	Q Science (General) QH Natural history
Online Access:	http://eprints.um.edu.my/18592/1/APJMBB2001PFGEPasteurella.pdf http://eprints.um.edu.my/18592/ http://www.msmbb.my/index.php/publication
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