Staff View: Screening of commercial meat products from supermarket chains for feline derivatives using SP-PCR-RLFP and lab-on-a-chip

Screening of commercial meat products from supermarket chains for feline derivatives using SP-PCR-RLFP and lab-on-a-chip

Determination of feline meat in food products is an important issue for social, health, economic and religious concern. Hence this paper documented the application of species specific polymerase chain reaction-restriction fragment length polymorphism (SP-PCR-RFLP) assay targeting a short-fragments (...

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Main Authors:	Amin, Md Al, Rahman, Md Mahfujur, Ahmad Razimi, Mohd Shahril, Chowdhury, Zaira Zaman, Md Hussain, Muhammad Nasri, Mohd Desa, Mohd Nasir
Format:	Article
Language:	English
Published:	Elsevier Ltd. 2020
Subjects:	HB Economic Theory
Online Access:	http://repo.uum.edu.my/27160/1/alamin2020.pdf http://repo.uum.edu.my/27160/ http://doi.org/10.1016/j.jfca.2020.103565
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id	my.uum.repo.27160
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spelling	my.uum.repo.271602020-07-01T07:03:43Z http://repo.uum.edu.my/27160/ Screening of commercial meat products from supermarket chains for feline derivatives using SP-PCR-RLFP and lab-on-a-chip Amin, Md Al Rahman, Md Mahfujur Ahmad Razimi, Mohd Shahril Chowdhury, Zaira Zaman Md Hussain, Muhammad Nasri Mohd Desa, Mohd Nasir HB Economic Theory Determination of feline meat in food products is an important issue for social, health, economic and religious concern. Hence this paper documented the application of species specific polymerase chain reaction-restriction fragment length polymorphism (SP-PCR-RFLP) assay targeting a short-fragments (69 bp) of mitochondrial cytochrome b (cytb) gene to screen feline meat in commercial meat products using lab-on-a-chip. The SP-PCR assay proved its specificity theoretically and experimentally while testing with different common animal, aquatic and plant species of DNA. The feline specific (69 bp, 43- and 26-bp) characteristic molecular DNA pattern was observed by SP-PCR and RFLP analysis. For assay performance, it was tested in three different types of commercial dummy meat products such as frankfurters, nuggets and meatballs and digested with AluI-restriction enzyme. The highest sensitivity of the assay using lab-on-a-chip was as low as 0.1 pg or 0.01 % (w/w) in commercial dummy meat products. We have also applied this assay to screen three important commercial meat products of six different brand from six supermarket chains located at three different states of Malaysia. Thus total 378 samples were tested to validate the specificity, sensitivity, stability of the assay and utilization of it for commercial meat product screening. Elsevier Ltd. 2020 Article PeerReviewed application/pdf en http://repo.uum.edu.my/27160/1/alamin2020.pdf Amin, Md Al and Rahman, Md Mahfujur and Ahmad Razimi, Mohd Shahril and Chowdhury, Zaira Zaman and Md Hussain, Muhammad Nasri and Mohd Desa, Mohd Nasir (2020) Screening of commercial meat products from supermarket chains for feline derivatives using SP-PCR-RLFP and lab-on-a-chip. Journal of Food Composition and Analysis, 92. pp. 1-32. ISSN 08891575 http://doi.org/10.1016/j.jfca.2020.103565 doi:10.1016/j.jfca.2020.103565
institution	Universiti Utara Malaysia
building	UUM Library
collection	Institutional Repository
continent	Asia
country	Malaysia
content_provider	Universiti Utara Malaysia
content_source	UUM Institutional Repository
url_provider	http://repo.uum.edu.my/
language	English
topic	HB Economic Theory
spellingShingle	HB Economic Theory Amin, Md Al Rahman, Md Mahfujur Ahmad Razimi, Mohd Shahril Chowdhury, Zaira Zaman Md Hussain, Muhammad Nasri Mohd Desa, Mohd Nasir Screening of commercial meat products from supermarket chains for feline derivatives using SP-PCR-RLFP and lab-on-a-chip
description	Determination of feline meat in food products is an important issue for social, health, economic and religious concern. Hence this paper documented the application of species specific polymerase chain reaction-restriction fragment length polymorphism (SP-PCR-RFLP) assay targeting a short-fragments (69 bp) of mitochondrial cytochrome b (cytb) gene to screen feline meat in commercial meat products using lab-on-a-chip. The SP-PCR assay proved its specificity theoretically and experimentally while testing with different common animal, aquatic and plant species of DNA. The feline specific (69 bp, 43- and 26-bp) characteristic molecular DNA pattern was observed by SP-PCR and RFLP analysis. For assay performance, it was tested in three different types of commercial dummy meat products such as frankfurters, nuggets and meatballs and digested with AluI-restriction enzyme. The highest sensitivity of the assay using lab-on-a-chip was as low as 0.1 pg or 0.01 % (w/w) in commercial dummy meat products. We have also applied this assay to screen three important commercial meat products of six different brand from six supermarket chains located at three different states of Malaysia. Thus total 378 samples were tested to validate the specificity, sensitivity, stability of the assay and utilization of it for commercial meat product screening.
format	Article
author	Amin, Md Al Rahman, Md Mahfujur Ahmad Razimi, Mohd Shahril Chowdhury, Zaira Zaman Md Hussain, Muhammad Nasri Mohd Desa, Mohd Nasir
author_facet	Amin, Md Al Rahman, Md Mahfujur Ahmad Razimi, Mohd Shahril Chowdhury, Zaira Zaman Md Hussain, Muhammad Nasri Mohd Desa, Mohd Nasir
author_sort	Amin, Md Al
title	Screening of commercial meat products from supermarket chains for feline derivatives using SP-PCR-RLFP and lab-on-a-chip
title_short	Screening of commercial meat products from supermarket chains for feline derivatives using SP-PCR-RLFP and lab-on-a-chip
title_full	Screening of commercial meat products from supermarket chains for feline derivatives using SP-PCR-RLFP and lab-on-a-chip
title_fullStr	Screening of commercial meat products from supermarket chains for feline derivatives using SP-PCR-RLFP and lab-on-a-chip
title_full_unstemmed	Screening of commercial meat products from supermarket chains for feline derivatives using SP-PCR-RLFP and lab-on-a-chip
title_sort	screening of commercial meat products from supermarket chains for feline derivatives using sp-pcr-rlfp and lab-on-a-chip
publisher	Elsevier Ltd.
publishDate	2020
url	http://repo.uum.edu.my/27160/1/alamin2020.pdf http://repo.uum.edu.my/27160/ http://doi.org/10.1016/j.jfca.2020.103565
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score	13.211869

Screening of commercial meat products from supermarket chains for feline derivatives using SP-PCR-RLFP and lab-on-a-chip

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