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Quantitative analysis of palm carotene using fourier transform infrared and near infrared spectroscopy

β-Carotene content is usually determined by using ultraviolet (UV)-visible spectrophotometry at 446 nm.In this study, two spectroscopic techniques, namely, Fourier transform infrared (FTIR) and near infrared (NIR) spectroscopy, have been investigated and compared to UV-visible spectrophotometry to...

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Main Authors: Moh, M. H., Man, Y. B. Che, Badlishah, Shahrizal, Jinap, S., Saad, M. S., Abdullah, W. J. W.
Format: Article
Language:English
Published: OCS Press 1999
Subjects:
QA Mathematics
Online Access:http://repo.uum.edu.my/10709/1/fe.pdf
http://repo.uum.edu.my/10709/
http://dx.doi.org/10.1007/s11746-999-0226-9
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spelling my.uum.repo.107092014-04-21T08:23:25Z http://repo.uum.edu.my/10709/ Quantitative analysis of palm carotene using fourier transform infrared and near infrared spectroscopy Moh, M. H. Man, Y. B. Che Badlishah, Shahrizal Jinap, S. Saad, M. S. Abdullah, W. J. W. QA Mathematics β-Carotene content is usually determined by using ultraviolet (UV)-visible spectrophotometry at 446 nm.In this study, two spectroscopic techniques, namely, Fourier transform infrared (FTIR) and near infrared (NIR) spectroscopy, have been investigated and compared to UV-visible spectrophotometry to measure the β-carotene content of crude palm oil (CPO).Calibration curves ranging from 200 to 800 ppm were prepared by extracting β-carotene from original CPO using open-column chromatography.Separate partial least squares calibration models were developed for predicting β-carotene based on the spectral region from 976 to 926 cm−1 for FTIR spectroscopy and 546 to 819 nm for NIR spectroscopy.The correlation coefficient (R 2) and standard error of calibration obtained were 0.972 and 25.2 for FTIR and 0.952 and 23.6 for NIR techniques, respectively.The validation set gave R 2 of 0.951 with standard error of performance (SEP) of 25.78 for FTIR technique and R 2 of 0.979 with SEP of 19.96 for NIR technique.The overall reproducibility and accuracy did not give comparable results to that of spectrophotometric method; however, the standard deviation of prediction was still within ±5% β-carotene content over the range tested.Because of their rapidness and simplicity, both FTIR and NIR techniques provide alternative means of measuring β-carotene content in CPO.In addition, these two spectroscopic techniques are environmentally friendly since no solvent is involved. OCS Press 1999 Article PeerReviewed application/pdf en http://repo.uum.edu.my/10709/1/fe.pdf Moh, M. H. and Man, Y. B. Che and Badlishah, Shahrizal and Jinap, S. and Saad, M. S. and Abdullah, W. J. W. (1999) Quantitative analysis of palm carotene using fourier transform infrared and near infrared spectroscopy. Journal of the American Oil Chemists' Society, 76 (2). pp. 249-254. ISSN 0003-021X http://dx.doi.org/10.1007/s11746-999-0226-9 doi:10.1007/s11746-999-0226-9
institution Universiti Utara Malaysia
building UUM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Utara Malaysia
content_source UUM Institutionali Repository
url_provider http://repo.uum.edu.my/
language English
topic QA Mathematics
spellingShingle QA Mathematics
Moh, M. H.
Man, Y. B. Che
Badlishah, Shahrizal
Jinap, S.
Saad, M. S.
Abdullah, W. J. W.
Quantitative analysis of palm carotene using fourier transform infrared and near infrared spectroscopy
description β-Carotene content is usually determined by using ultraviolet (UV)-visible spectrophotometry at 446 nm.In this study, two spectroscopic techniques, namely, Fourier transform infrared (FTIR) and near infrared (NIR) spectroscopy, have been investigated and compared to UV-visible spectrophotometry to measure the β-carotene content of crude palm oil (CPO).Calibration curves ranging from 200 to 800 ppm were prepared by extracting β-carotene from original CPO using open-column chromatography.Separate partial least squares calibration models were developed for predicting β-carotene based on the spectral region from 976 to 926 cm−1 for FTIR spectroscopy and 546 to 819 nm for NIR spectroscopy.The correlation coefficient (R 2) and standard error of calibration obtained were 0.972 and 25.2 for FTIR and 0.952 and 23.6 for NIR techniques, respectively.The validation set gave R 2 of 0.951 with standard error of performance (SEP) of 25.78 for FTIR technique and R 2 of 0.979 with SEP of 19.96 for NIR technique.The overall reproducibility and accuracy did not give comparable results to that of spectrophotometric method; however, the standard deviation of prediction was still within ±5% β-carotene content over the range tested.Because of their rapidness and simplicity, both FTIR and NIR techniques provide alternative means of measuring β-carotene content in CPO.In addition, these two spectroscopic techniques are environmentally friendly since no solvent is involved.
format Article
author Moh, M. H.
Man, Y. B. Che
Badlishah, Shahrizal
Jinap, S.
Saad, M. S.
Abdullah, W. J. W.
author_facet Moh, M. H.
Man, Y. B. Che
Badlishah, Shahrizal
Jinap, S.
Saad, M. S.
Abdullah, W. J. W.
author_sort Moh, M. H.
title Quantitative analysis of palm carotene using fourier transform infrared and near infrared spectroscopy
title_short Quantitative analysis of palm carotene using fourier transform infrared and near infrared spectroscopy
title_full Quantitative analysis of palm carotene using fourier transform infrared and near infrared spectroscopy
title_fullStr Quantitative analysis of palm carotene using fourier transform infrared and near infrared spectroscopy
title_full_unstemmed Quantitative analysis of palm carotene using fourier transform infrared and near infrared spectroscopy
title_sort quantitative analysis of palm carotene using fourier transform infrared and near infrared spectroscopy
publisher OCS Press
publishDate 1999
url http://repo.uum.edu.my/10709/1/fe.pdf
http://repo.uum.edu.my/10709/
http://dx.doi.org/10.1007/s11746-999-0226-9
_version_ 1644280438893576192
score 13.211869

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