Cover Image

Transdermal Delivery of Antigenic Protein Using Ionic Liquid-Based Nanocarriers for Tumor Immunotherapy

Transdermal drug delivery systems (TDDSs) may be useful for preventing various diseases including cancer. However, the stratum corneum (SC) inhibits the permeation of foreign particles into the skin. To obtain an effective TDDS, we developed a protein-containing nanocarrier (PCNC) comprising an anti...

Full description

Saved in:
Bibliographic Details
Main Authors: Uddin, S., Islam, M.R., Md Moshikur, R., Wakabayashi, R., Kamiya, N., Moniruzzaman, M., Goto, M.
Format: Article
Published: American Chemical Society 2022
Online Access:https://www.scopus.com/inward/record.uri?eid=2-s2.0-85129263771&doi=10.1021%2facsabm.2c00061&partnerID=40&md5=c64808a3ba3cf0acf66afd0a50e543e3
http://eprints.utp.edu.my/33236/
Tags: Add Tag
No Tags, Be the first to tag this record!
id my.utp.eprints.33236
record_format eprints
spelling my.utp.eprints.332362022-07-06T08:25:58Z Transdermal Delivery of Antigenic Protein Using Ionic Liquid-Based Nanocarriers for Tumor Immunotherapy Uddin, S. Islam, M.R. Md Moshikur, R. Wakabayashi, R. Kamiya, N. Moniruzzaman, M. Goto, M. Transdermal drug delivery systems (TDDSs) may be useful for preventing various diseases including cancer. However, the stratum corneum (SC) inhibits the permeation of foreign particles into the skin. To obtain an effective TDDS, we developed a protein-containing nanocarrier (PCNC) comprising an antigenic protein (ovalbumin/OVA) stabilized by a combination of surfactants, i.e., a lipid-based surface-active ionic liquid and Tween-80. The PCNC was lyophilized to remove water and cyclohexane and then dispersed in isopropyl myristate. It is biocompatible both in vitro and in vivo, and is suitable for use in a therapeutic TDDS. The skin permeability of the PCNC was significantly (p < 0.0001) enhanced, and the transdermal distribution and transdermal flux of the OVA delivery system were 25 and 28 times greater, respectively, than those of its aqueous formulation. The PCNC disrupted the order of lipid orientation in the skin's SC and increased intercellular protein delivery. It demonstrated effective antitumor activity, drastically (p < 0.001) suppressed tumor growth, increased mouse survival rates, and significantly (p < 0.001) stimulated the OVA-specific tumor immune response. The PCNC also increased the number of cytotoxic T cells expressing CD8 antibodies on their surfaces (CD8 + T-cells) in the tumor microenvironment. These findings suggest that PCNCs may be promising biocompatible carriers for transdermal antigenic protein delivery in tumor immunotherapy. © 2022 American Chemical Society. American Chemical Society 2022 Article NonPeerReviewed https://www.scopus.com/inward/record.uri?eid=2-s2.0-85129263771&doi=10.1021%2facsabm.2c00061&partnerID=40&md5=c64808a3ba3cf0acf66afd0a50e543e3 Uddin, S. and Islam, M.R. and Md Moshikur, R. and Wakabayashi, R. and Kamiya, N. and Moniruzzaman, M. and Goto, M. (2022) Transdermal Delivery of Antigenic Protein Using Ionic Liquid-Based Nanocarriers for Tumor Immunotherapy. ACS Applied Bio Materials . http://eprints.utp.edu.my/33236/
institution Universiti Teknologi Petronas
building UTP Resource Centre
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Teknologi Petronas
content_source UTP Institutional Repository
url_provider http://eprints.utp.edu.my/
description Transdermal drug delivery systems (TDDSs) may be useful for preventing various diseases including cancer. However, the stratum corneum (SC) inhibits the permeation of foreign particles into the skin. To obtain an effective TDDS, we developed a protein-containing nanocarrier (PCNC) comprising an antigenic protein (ovalbumin/OVA) stabilized by a combination of surfactants, i.e., a lipid-based surface-active ionic liquid and Tween-80. The PCNC was lyophilized to remove water and cyclohexane and then dispersed in isopropyl myristate. It is biocompatible both in vitro and in vivo, and is suitable for use in a therapeutic TDDS. The skin permeability of the PCNC was significantly (p < 0.0001) enhanced, and the transdermal distribution and transdermal flux of the OVA delivery system were 25 and 28 times greater, respectively, than those of its aqueous formulation. The PCNC disrupted the order of lipid orientation in the skin's SC and increased intercellular protein delivery. It demonstrated effective antitumor activity, drastically (p < 0.001) suppressed tumor growth, increased mouse survival rates, and significantly (p < 0.001) stimulated the OVA-specific tumor immune response. The PCNC also increased the number of cytotoxic T cells expressing CD8 antibodies on their surfaces (CD8 + T-cells) in the tumor microenvironment. These findings suggest that PCNCs may be promising biocompatible carriers for transdermal antigenic protein delivery in tumor immunotherapy. © 2022 American Chemical Society.
format Article
author Uddin, S.
Islam, M.R.
Md Moshikur, R.
Wakabayashi, R.
Kamiya, N.
Moniruzzaman, M.
Goto, M.
spellingShingle Uddin, S.
Islam, M.R.
Md Moshikur, R.
Wakabayashi, R.
Kamiya, N.
Moniruzzaman, M.
Goto, M.
Transdermal Delivery of Antigenic Protein Using Ionic Liquid-Based Nanocarriers for Tumor Immunotherapy
author_facet Uddin, S.
Islam, M.R.
Md Moshikur, R.
Wakabayashi, R.
Kamiya, N.
Moniruzzaman, M.
Goto, M.
author_sort Uddin, S.
title Transdermal Delivery of Antigenic Protein Using Ionic Liquid-Based Nanocarriers for Tumor Immunotherapy
title_short Transdermal Delivery of Antigenic Protein Using Ionic Liquid-Based Nanocarriers for Tumor Immunotherapy
title_full Transdermal Delivery of Antigenic Protein Using Ionic Liquid-Based Nanocarriers for Tumor Immunotherapy
title_fullStr Transdermal Delivery of Antigenic Protein Using Ionic Liquid-Based Nanocarriers for Tumor Immunotherapy
title_full_unstemmed Transdermal Delivery of Antigenic Protein Using Ionic Liquid-Based Nanocarriers for Tumor Immunotherapy
title_sort transdermal delivery of antigenic protein using ionic liquid-based nanocarriers for tumor immunotherapy
publisher American Chemical Society
publishDate 2022
url https://www.scopus.com/inward/record.uri?eid=2-s2.0-85129263771&doi=10.1021%2facsabm.2c00061&partnerID=40&md5=c64808a3ba3cf0acf66afd0a50e543e3
http://eprints.utp.edu.my/33236/
_version_ 1738657474331803648
score 13.222552

Similar Items