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Design of Aqueous Two-Phase Protein Separation Systems

Aqueous Two-Phase System (ATPS), despite its potential as a bulk protein separation technique that offers high yield, low cost and mild environment, has limited application at industrial scale. The work proposes an integrated framework that calculates flowsheet design for the application of ATPS to...

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Main Author: Ahmad, Murni M
Format: Conference or Workshop Item
Published: 2005
Subjects:
TP Chemical technology
Online Access:http://eprints.utp.edu.my/3256/1/Design_of_Aqueous_Two_Phase_Protein_Separation_Systems_%28ICBP_2005%29.pdf
http://eprints.utp.edu.my/3256/
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spelling my.utp.eprints.32562017-01-19T08:27:28Z Design of Aqueous Two-Phase Protein Separation Systems Ahmad, Murni M TP Chemical technology Aqueous Two-Phase System (ATPS), despite its potential as a bulk protein separation technique that offers high yield, low cost and mild environment, has limited application at industrial scale. The work proposes an integrated framework that calculates flowsheet design for the application of ATPS to extract a target protein from cell lysate. The framework integrates the liquid-liquid equilibrium calculation based on Flory-Huggins Model and the mass balance calculation for the flowsheet. The applicability of the framework is demonstrated using a case study on a multi-stage extraction of phosphofructokinase and ovalbumin using PEG6000-DxT500 and PEG6000-Na3PO4 ATPSs. 2005-06 Conference or Workshop Item PeerReviewed application/pdf http://eprints.utp.edu.my/3256/1/Design_of_Aqueous_Two_Phase_Protein_Separation_Systems_%28ICBP_2005%29.pdf Ahmad, Murni M (2005) Design of Aqueous Two-Phase Protein Separation Systems. In: International Conference on Biopartitioning and Purification (BPP 2005), June 2005, Amsterdam, Netherlands. http://eprints.utp.edu.my/3256/
institution Universiti Teknologi Petronas
building UTP Resource Centre
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Teknologi Petronas
content_source UTP Institutional Repository
url_provider http://eprints.utp.edu.my/
topic TP Chemical technology
spellingShingle TP Chemical technology
Ahmad, Murni M
Design of Aqueous Two-Phase Protein Separation Systems
description Aqueous Two-Phase System (ATPS), despite its potential as a bulk protein separation technique that offers high yield, low cost and mild environment, has limited application at industrial scale. The work proposes an integrated framework that calculates flowsheet design for the application of ATPS to extract a target protein from cell lysate. The framework integrates the liquid-liquid equilibrium calculation based on Flory-Huggins Model and the mass balance calculation for the flowsheet. The applicability of the framework is demonstrated using a case study on a multi-stage extraction of phosphofructokinase and ovalbumin using PEG6000-DxT500 and PEG6000-Na3PO4 ATPSs.
format Conference or Workshop Item
author Ahmad, Murni M
author_facet Ahmad, Murni M
author_sort Ahmad, Murni M
title Design of Aqueous Two-Phase Protein Separation Systems
title_short Design of Aqueous Two-Phase Protein Separation Systems
title_full Design of Aqueous Two-Phase Protein Separation Systems
title_fullStr Design of Aqueous Two-Phase Protein Separation Systems
title_full_unstemmed Design of Aqueous Two-Phase Protein Separation Systems
title_sort design of aqueous two-phase protein separation systems
publishDate 2005
url http://eprints.utp.edu.my/3256/1/Design_of_Aqueous_Two_Phase_Protein_Separation_Systems_%28ICBP_2005%29.pdf
http://eprints.utp.edu.my/3256/
_version_ 1738655256310448128
score 13.211869

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